Maturation and Migration of Murine Cd4 Single Positive Thymocytes and Thymic Emigrants

Xu, Xi; Ge, Qing

doi:10.5936/csbj.201403003

Cited by 9 publications

(6 citation statements)

References 132 publications

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“…However, recent studies have revealed that newly generated SP thymocytes undergo further developmental programme in the thymic medulla before they follow a tightly regulated emigrating process to become RTEs . We detected TRECs to determinate RTEs that is often used as a marker to predict the status of thymopoiesis . As shown in Fig.…”

Section: Resultsmentioning

confidence: 92%

Thyrotropin Alters T Cell Development in the Thymus in Subclinical Hypothyroidism Mouse Model

Zhao

Zhang

et al. 2017

Scand J Immunol

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Subclinical hypothyroidism (SCH) is highly prevalent in the general population and is associated with potential deleterious effects. Although developing T cells express thyroid-stimulating hormone receptor (TSH-R), the changes of T cell development in thymus in SCH have not been fully clarified. SCH mouse model, which is characterized by elevated serum TSH but similar thyroid hormone levels, was used to study the role of TSH in T cell development. Thymus weight of SCH mice increased 18% compared with controls. Importantly, the frequencies of CD4 + and CD8 + single-positive (SP) thymocytes increased 38% and 44%, respectively. We demonstrated that TSH protected thymocytes from apoptosis as evidenced by a significant decrease of Annexin V-positive thymocytes in SCH mice. Further analysis showed that extracellular-regulated kinases (ERK) 1/2 in thymus were activated in SCH mice. With analysis of T cell receptor excision circles (TREC), we found that TSH increased recent thymic emigrants (RTE) in spleen tissue in SCH mice. Thus, these results suggest that TSH promoted T cell development and enhanced the thymic recent output in SCH mice, possibly by suppression of apoptosis of thymocytes, indicating that modification of the ERK signalling pathways.

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Section: Resultsmentioning

confidence: 92%

Thyrotropin Alters T Cell Development in the Thymus in Subclinical Hypothyroidism Mouse Model

Zhao

Zhang

et al. 2017

Scand J Immunol

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“…Following maturation, thymocytes exist in the thymus as latestage thymocytes before they egress the thymus as recent thymic emigrants (32). To assess a possible role for coronin 1 at later developmental stages, the levels of cell surface markers that define this cell population, in particular CD62L, Qa2, and CD24, were assessed.…”

Section: Resultsmentioning

confidence: 99%

A Coronin 1–Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment

Lang

Mori

Ruer-Laventie

et al. 2017

The Journal of Immunology

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Following thymic maturation, T cells egress as recent thymic emigrants to peripheral lymphoid organs where they undergo an additional maturation step to mature naive T cells that circulate through secondary lymphoid organs ready to be activated upon pathogenic challenges. Thymic maturation and peripheral T cell survival depend on several signaling cascades, but whether a dedicated mechanism exists that exclusively regulates homeostasis of mature naive T cells without affecting thymocytes and/or recent thymic emigrants remains unknown. In this article, we provide evidence for a specific and exclusive role of the WD repeat containing protein coronin 1 in the maintenance of naive T cells in peripheral lymphoid organs. We show that coronin 1 is dispensable for thymocyte survival and development, egress from the thymus, and survival of recent thymic emigrants. Importantly, coronin 1-deficient mice possessed comparable levels of peripheral T cells within the first 2 wk after birth but failed to populate the peripheral T cell compartment at later stages. Furthermore, dendritic cell- and IL-2/7-dependent T cell survival was found to be independent of coronin 1. Together, these results suggest the existence of a hitherto unrecognized coronin 1-dependent decision switch early during life that is responsible for peripheral naive T cell survival and homeostasis.

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“…Thymocytes will then differentiate into single-positive CD4 + T cells or CD8 + T cells if they respond to MHC-II or MHC-I molecules, respectively [93]. Upon single-positive selection, these thymocytes migrate to secondary locations such as the spleen and lymph nodes [94, 95]. The increase in single-positive CD8 + thymocytes following oxaliplatin may suggest the enhanced recruitment of cytotoxic T cells to the periphery.…”

Section: Discussionmentioning

confidence: 99%

Oxaliplatin Treatment Alters Systemic Immune Responses

Stojanovska

Prakash

McQuade

et al. 2019

BioMed Research International

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Purpose. Oxaliplatin is a platinum-based chemotherapeutic agent demonstrating significant antitumor efficacy. Unlike conventional anticancer agents which are immunosuppressive, oxaliplatin has the capacity to stimulate immunological effects in response to the presentation of damage associated molecular patterns (DAMPs) elicited upon cell death. However, the effects of oxaliplatin treatment on systemic immune responses remain largely unknown. Aims of this study were to investigate the effects of oxaliplatin treatment on the proportions of (1) splenic T cells, B cells, macrophages, pro-/anti-inflammatory cytokines, gene expression of splenic cytokines, chemokines, and mediators; (2) double-positive and single-positive CD4+and CD8+T thymocytes; (3) bone-marrow hematopoietic stem and progenitor cells.Methods. Male BALB/c mice received intraperitoneal injections of oxaliplatin (3mg/kg/d) or sterile water tri-weekly for 2 weeks. Leukocyte populations within the spleen, thymus, and bone-marrow were assessed using flow cytometry. RT-PCR was performed to characterise changes in splenic inflammation-associated genes.Results. Oxaliplatin treatment reduced spleen size and cellularity (CD45+cells), increased the proportion of CD4+, CD8+, and Treg cells, and elevated TNF-αexpression. Oxaliplatin was selectively cytotoxic to B cells but had no effect on splenic macrophages. Oxaliplatin treatment altered the gene expression of several cytokines, chemokines, and cell mediators. Oxaliplatin did not deplete double-positive thymocytes but increased the single-positive CD8+subset. There was also an increase in activated (CD69+) CD8+T cells. Bone-marrow hematopoietic progenitor pool was demonstrably normal following oxaliplatin treatment when compared to the vehicle-treated cohort.Conclusion. Oxaliplatin does not cause systemic immunosuppression and, instead, has the capacity to induce beneficial antitumor immune responses.

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Maturation and Migration of Murine Cd4 Single Positive Thymocytes and Thymic Emigrants

Cited by 9 publications

References 132 publications

Thyrotropin Alters T Cell Development in the Thymus in Subclinical Hypothyroidism Mouse Model

Thyrotropin Alters T Cell Development in the Thymus in Subclinical Hypothyroidism Mouse Model

A Coronin 1–Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment

Oxaliplatin Treatment Alters Systemic Immune Responses

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