Maturation and secretion of a serine proteinase is associated with events of late microsporogenesis

Aa, Taylor; Horsch, Andrea; Rzepczyk, Annette; Ca, Hasenkampf; Riggs, C. Daniel

doi:10.1046/j.1365-313x.1997.12061261.x

Cited by 72 publications

(67 citation statements)

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“…A number of stage-specific genes have been isolated and functionally characterized with respect to their role in reproductive biology in Arabidopsis and several other plant species (reviewed in Taylor et al 1997;Sanders et al 1999;reviewed in Ma 2005). However, the genome-wide information of these specific gene expression patterns in relation to their developmental stages is still very limited.…”

Section: Introductionmentioning

confidence: 99%

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Kang

Zhang

Bonnema³

et al. 2007

Plant Mol Biol

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The dominant male sterility gene Ms-cd1 is identified in Brassica oleracea. Electron microscopical observations revealed that abortion of pollen development starts after tetrad formation. This important male sterility phenotype is characterized by lack of degradation of the primary pollen mother cell (PMC) wall and delayed degradation of callose surrounding the tetrads and thus arrest of microspore release. Gene expression of the male sterile and fertile buds was analyzed by heterologous hybridization of Brassica oleracea cRNA onto an Arabidopsis whole genome oligonucleotide microarray. A total of 277 suppressed genes including 40 kinase-, 32 cell wall modification and 29 transport related genes were found to be significantly down regulated [3-fold in the male sterile mutant. The vast majority of the differentially expressed transcripts are found to present late pollen stage specific genes. Kinase genes, cell wall modification genes and ion transport genes were greatly over-represented when compared to their percentage of all flower bud expressed genes and represent 36.5% of the genes suppressed by Ms-cd1. Our results also suggest that Ms-cd1 may blocks an anther developmental pathway with a small number of genes suppressed in tapetum cells which prevent the degradation of callose and PMC wall, which further leads to the suppression of a large number of genes involved in signaling pathways, cell wall modification and ion transport in pollen grains.

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Section: Introductionmentioning

confidence: 99%

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Kang

Zhang

Bonnema³

et al. 2007

Plant Mol Biol

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“…In situ hybridization revealed that ag12 genes are expressed in Frankia-infected cells of A. glutinosa nodules. While subtilisin-like proteases of the Kex2-family have been shown to play an important role in the secretory pathway in yeast and mammalia (Vierstra 1996), only a few examples of subtilisin-like proteases from higher plants have been published (Kobayashi et al 1994;Yamagata et al 1994;Ribeiro et al 1995;Tornero et al 1996;Taylor et al 1997). The plant subtilases identified seem to be involved in microsporogenesis, fruit ripening, the infection of nodule cells by Frankia, and pathogen response, respectively.…”

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confidence: 99%

“…The plant subtilases identified seem to be involved in microsporogenesis, fruit ripening, the infection of nodule cells by Frankia, and pathogen response, respectively. They contain N-terminal signal peptides, suggesting that they are secreted to the plant extracellular matrix where they are presumably involved in the processing of pericellular substrates (Yamagata et al 1994;Ribeiro et al 1995;Tornero et al 1996;Taylor et al 1997).…”

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confidence: 99%

Characterization of a Casuarina glauca Nodule-Specific Subtilisin-like Protease Gene, a Homolog of Alnus glutinosa ag12

Laplaze¹,

Ribeiro²,

Franche³

et al. 2000

MPMI

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In search of plant genes expressed during early interactions between Casuarina glauca and Frankia, we have isolated and characterized a C. glauca gene that has strong homology to subtilisin-like protease gene families of several plants including the actinorhizal nodulin gene ag12 of another actinorhizal plant, Alnus glutinosa. Based on the expression pattern of cg12 in the course of nodule development, it represents an early actinorhizal nodulin gene. Our results suggest that subtilisin-like proteases may be a common element in the process of infection of plant cells by Frankia in both Betulaceae (Alnus glutinosa) and Casuarinaceae (Casuarina glauca) symbioses.Additional keyword: symbiosis.

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“…Total protein was isolated from 100 mg of Stage 2 anthers (Koltunow et al 1990) of tobacco plants using NRD buffer {20 mM Tris (pH 8.6), 10% glycerol, 1% SDS} (Taylor et al 1997;DeGuzman and Riggs 2000). The isolated proteins were quantified using a method by Lowry et al (1951).…”

Section: Protein Isolation and Zymogram Analysismentioning

confidence: 99%

“…observed during pollen development (Taylor et al 1997), similarly in Lilium, the serine proteinase LIM9 is secreted into anther locules (Radlowski et al 1996). Recently a gene, OsSIZ1, a small ubiquitin-related modifier (SUMO) E3 ligase that helps in anchoring SUMO to protein substrates for modifications has been shown to be developmentally regulated in rice reproductive tissues (Wang et al 2010).…”

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confidence: 97%

Expression of Aprotinin in Anther Causes Male Sterility in Tobacco var Petit havana

et al. 2011

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Expression of many proteinases has been documented during anther development.Although their roles are not completely understood, their inhibition could possibly result in impairment of anther development leading to male sterility. We proposed that such an impairment of anther development can be engineered in plants and explored for hybrid seed production. Here we report that anther specific expression of Aprotinin gene (serine proteinase inhibitor) in tobacco has resulted in male sterility. Southern analysis of the transgenic plants revealed the integration of Aprotinin gene and zymogram analysis confirmed the expression of APROTININ in the anthers of the transgenic plants. Transverse sections of anthers of transgenic male sterile plants showed damage to the tapetum, the site of tissue-specific expression of the TA29 promoter. The pollen germination in the transgenic plants ranged between 2% to 65% that confirmed the impairment in pollen production leading to male sterility and low seed yield. Thus inhibition of serine proteinases that are expressed during anther development resulted in impaired pollen production and male sterility though more work is required to elucidate the exact role of these proteinases.

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Maturation and secretion of a serine proteinase is associated with events of late microsporogenesis

Cited by 72 publications

References 0 publications

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Characterization of a Casuarina glauca Nodule-Specific Subtilisin-like Protease Gene, a Homolog of Alnus glutinosa ag12

Expression of Aprotinin in Anther Causes Male Sterility in Tobacco var Petit havana

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