Maturation, fertilization and complete development of porcine oocytes matured under different systems

Coy, Pilar; Ruiz, Salvador; Romar, Raquel; Campos, I.; Gadea, J.

doi:10.1016/s0093-691x(99)00028-x

Cited by 31 publications

(22 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it is well documented that nuclear and cytoplasmic maturation are not always correlated (Coy et al 1999). The resumption of meiosis in oocytes after removal from roscovitine, presumably re-starting the activation of MPF, is not a prerequisite to ensure the cytoplasmic maturation of such oocytes.…”

Section: Discussionmentioning

confidence: 96%

“…The resumption of meiosis in oocytes after removal from roscovitine, presumably re-starting the activation of MPF, is not a prerequisite to ensure the cytoplasmic maturation of such oocytes. On the contrary, intracellular GSH content and the ability of oocytes to form a male pronucleus are commonly accepted parameters for assessing cytoplasmic maturation of porcine oocytes (Funahashi et al 1995, Sawai et al 1997, Coy et al 1999. GSH is produced via the g-glutamyl cycle and is dependent on the amount of cysteine available to the cell (Meister & Tate 1976).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Coy¹,

Romar²,

Ruiz³

et al. 2005

Reproduction

View full text Add to dashboard Cite

The objectives of this study were to evaluate: (1) the nuclear maturation, (2) the intracellular glutathione (GSH) content, (3) the normality of fertilization and (4) full development after transplantation of embryos derived from porcine oocytes precultured with 50 mmol/l roscovitine (an inhibitor of p34 cdc2 /cyclin B kinase) for 22 h. After treatment with roscovitine, the nuclear configuration of oocytes (Hoechst staining) was comparable with those examined just after collection: the majority of oocytes were arrested at the germinal vesicle (GV) 1 stage (63.2%). Roscovitine-treated oocytes progressed through meiosis to the metaphase II stage in a conventional step-wise in vitro maturation (IVM) program for 44 h in a proportion similar to control ones (> 85.0%). When roscovitine-treated oocytes and non-treated oocytes were matured for 44 h and then co-cultured with fresh spermatozoa for 18 h, no differences were observed in oocyte penetrability, proportion of monospermic penetration and male pronuclear formation (>87%). Roscovitine increased the GSH synthesis in oocytes at 22 h, whereas, after 44 h, roscovitine-treated oocytes had similar amounts of GSH to non-treated oocytes. Finally, surgical transfer of zygotes at 22-24 h post-insemination, derived from roscovitine-treated oocytes, resulted in one pregnancy with 12 piglets born; control non-treated zygotes resulted in one pregnancy and 10 piglets born. The full-term developmental ability of mammalian oocytes pre-cultured with roscovitine prior to IVM is thereby demonstrated. This validation is important before the introduction of roscovitine into routine procedures.Reproduction (2005) 129 747-755

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Coy¹,

Romar²,

Ruiz³

et al. 2005

Reproduction

View full text Add to dashboard Cite

show abstract

“…Although undiluted FF is largely ineffective at supporting bovine oocyte maturation in vitro, it has been used to supplement media with varying degrees of success in equine, bovine and porcine systems, where oocyte maturation rates are affected by oestrous stage and follicle size (Gerritse et al 1988, Sirard & First 1988, Ayoub & Hunter 1993, Coy et al 1999, Aguilar et al 2001, Bøgh et al 2002, Avery et al 2003. This strategy exposes oocyte-cumulus complexes (OCCs) to a more 'physiological' -albeit undefinedhormonal and nutritional milieu.…”

Section: Introductionmentioning

confidence: 99%

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Orsi

Gopichandran²,

Leese³

et al. 2005

Reproduction

View full text Add to dashboard Cite

Bovine oocyte maturation in vitro frequently results in abnormal cytoplasmic maturation and failure to acquire developmental competence. This is, in part, likely to be due to the non-physiological nutritional milieu to which oocytes are exposed. Improvements in oocyte developmental potential may be achieved by modelling nutrient profiles on those of preovulatory follicular fluid (FF). However, little is known about fluctuations in FF nutrient levels according to follicle dominance and oestrous cyclicity. This study therefore characterised the carbohydrate and amino acid profile of FF according to these parameters, and compared preovulatory FF composition with that of maturation medium. Carbohydrate concentrations (n 5 121) were determined enzymatically whilst amino acid profiles (n 5 40) were determined by reverse-phase HPLC. Pyruvate and glucose concentrations were unaffected by follicle dominance, whereas Stage III -IV lactate profiles were higher in non-dominant FF (P < 0.01). While most dominant FF amino acid concentrations were affected by oestrous stage, only glutamate, alanine, leucine and lysine levels fluctuated in non-dominant FF. Glucose and lactate concentrations were significantly negatively correlated, whereas most amino acids were significantly positively correlated with each other. Maturation medium had higher pyruvate and lower lactate concentrations than preovulatory FF (P < 0.001), whereas glucose level was similar. All amino acid levels (except histidine, taurine, alanine and tryptophan) differed significantly between maturation medium and preovulatory FF. These data indicated that FF composition varies throughout the oestrous cycle. Preovulatory FF nutrient profile differed from that of maturation medium, perhaps accounting for the poor developmental competence of in vitro matured oocytes. These data may contribute to the formulation of a nutritionally more physiological maturation medium.

show abstract

“…Since this type of cloning requires a large number of recipient oocytes, the in vitro maturation system for preparing recipient oocytes that have high developmental competence is one of the important factors for the cloning technology. The developmental competence of porcine oocytes is affected by culture conditions during in vitro maturation (Funahashi et al, 1997;Coy et al, 1998;Yoshida et al, 1992a,b). In an attempt to produce cloned pigs from recipient oocytes in in vitro systems, Betthauser et al (2000) synchronized oocytes of their meiotic progression by dibutyryl cAMP (dbcAMP), the inhibitor of germinal vesicle breakdown.…”

Section: Introductionmentioning

confidence: 99%

Developmental Competence of Somatic Cell Nuclear Transfer Embryos Reconstructed from Oocytes Matured In Vitro with Follicle Shells in Miniature Pig

Hoshino

Uchida²,

Shimatsu³

et al. 2005

Cloning and Stem Cells

View full text Add to dashboard Cite

The developmental competence of domestic pig oocytes that were transferred to somatic cell nuclei of miniature pig was examined. A co-culture system of oocytes with follicle shells was used for the maturation of domestic pig oocytes in vitro. Co-cultured oocytes progressed to the metaphase II stage of meiosis more quickly and more synchronously than non co-cultured oocytes. Oocytes were enucleated and fused with fibroblast cells of Potbelly miniature pig at 48 h of maturation. The blastocyst formation rate of nuclear transfer (NT) embryos using cocultured oocytes (24%) was significantly higher (p < 0.05) than that of non-co-cultured oocytes (13%). Cleaved embryos at 48 h after nuclear transfer using co-cultured oocytes were transferred to the oviducts of 14 Göttingen miniature pigs and four Meishan pigs. Estrus of all Göttingens returned at around 20-31 days of pregnancy. Two of the four Meishans became pregnant. Three and two cloned piglets were born after modest number of embryo transfer (15 and 29 embryos transferred), respectively. These results indicated that oocytes co-cultured with follicle shells have a high developmental competence after nuclear transfer and result in full-term development after embryo transfer.

show abstract

Maturation, fertilization and complete development of porcine oocytes matured under different systems

Cited by 31 publications

References 45 publications

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Fluctuations in bovine ovarian follicular fluid composition throughout the oestrous cycle

Developmental Competence of Somatic Cell Nuclear Transfer Embryos Reconstructed from Oocytes Matured In Vitro with Follicle Shells in Miniature Pig

Contact Info

Product

Resources

About