Maturation of Neuromuscular Transmission During Early Development in Zebrafish

Nguyen, Peter V.; Aniksztejn, Laurent; Catarsi, Stefano; Drapeau, Pierre

doi:10.1152/jn.1999.81.6.2852

Cited by 34 publications

(55 citation statements)

References 42 publications

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“…The evoked synaptic currents showed remarkable synaptic speed as reflected in the decay time constants that averaged 0.64 ms at Ϫ50 mV. This speed of synaptic current was indicated in measurements of synaptic potentials (Buss and Drapeau, 2000) and in the decay of spontaneous synaptic currents (Nguyen et al, 1999;Luna et al, 2004). Now the decay times obtained for EPCs confirm the synaptic speed.…”

Section: Discussionmentioning

confidence: 52%

“…Both time points were measured using IGOR Pro software (WaveMetrics, Lake Oswego, OR) with NeuroMatic extension (Laboratory of Synaptic Transmission and Information Processing, University College London, London, UK). For miniature EPC (mEPC) analysis, events with 10 -90% rise times longer than 0.4 ms and under 50 pA in amplitude were excluded, because these slow and small events are electrotonic reflections of currents generated in neighboring muscle cells (Nguyen et al, 1999;Buss and Drapeau, 2000;Luna et al, 2004). All data represent mean Ϯ SD unless otherwise specified, and statistical significance was assessed by a two-tailed t test.…”

Section: Methodsmentioning

confidence: 99%

“…The target fast muscle cells in the ventral mid-region of 96 -144 hpf fish were reasonably electrically compact despite the presence of weak electrical coupling (Nguyen et al, 1999;Luna et al, 2004). Because of the small size of muscle, series resistance compensation resulted in no significant change in synaptic current waveform.…”

Section: Determination Of Quantal Featuresmentioning

confidence: 97%

“…We find that the fast skeletal muscle of the tail is small and electrically compact, facilitating an effective voltage clamp. So, in addition to spontaneous synaptic currents (Nguyen et al, 1999), larval zebrafish now offers a unique opportunity to examine the evoked end-plate current (EPC).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Paired Motor Neuron–Muscle Recordings in Zebrafish Test the Receptor Blockade Model for Shaping Synaptic Current

Hua¹,

Brehm²

2005

J. Neurosci.

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The transparent spinal cord and electrically compact fast muscle of zebrafish offer the first opportunity to perform simultaneous whole-cell patch-clamp recordings from both motor neuron and target skeletal muscle in situ. Our paired recordings reveal the fastest reported kinetics for both spontaneous and evoked synaptic currents at any synapse and a large quantal size that facilitates the resolution of spontaneous synaptic currents. We used this preparation to test the recent proposal that open channel block of the acetylcholine receptor by acetylcholine modulates the kinetics and timing of transmission between nerve and muscle in larval zebrafish (Legendre et al., 2000). Contrary to the predictions of this model, we find similar delay and onset kinetics of synaptic current at positive and negative muscle membrane potentials, even after inhibition of acetylcholinesterase. In contrast, blockade of motor neuron K channels by 4-aminopyridine prolonged the action potential and introduced a significant delay and slowing of evoked synaptic currents, demonstrating our ability to measured altered transmitter release with this system. We conclude that the kinetics of neuromuscular synaptic currents in zebrafish is not governed by receptor block.

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Section: Discussionmentioning

confidence: 52%

Section: Methodsmentioning

confidence: 99%

Section: Determination Of Quantal Featuresmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Paired Motor Neuron–Muscle Recordings in Zebrafish Test the Receptor Blockade Model for Shaping Synaptic Current

Hua¹,

Brehm²

2005

J. Neurosci.

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show abstract

“…1-4). However, the time course of larval ER and EW muscle miniature EPPs are similar (Buss and Drapeau 2000;Nguyen et al 1999), indicating that the rhythmic EPP summation was likely due to other factors. EPP summation could be due to EPPs originating from adjacent electrically coupled ER muscle fibers as EW fiber coupling is minimal at day 3, whereas ER fibers are extensively coupled (Buss and Drapeau 2000).…”

Section: Fictive Coiling and Swimming Behaviors Prior To Hatchingmentioning

confidence: 95%

Activation of Embryonic Red and White Muscle Fibers During Fictive Swimming in the Developing Zebrafish

Buss

Drapeau

2002

Journal of Neurophysiology

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Buss, Robert R. and Pierre Drapeau. Activation of embryonic red and white muscle fibers during fictive swimming in the developing zebrafish. J Neurophysiol 87: 1244 -1251, 2002; 10.1152/jn.00659.2001. Sub-threshold, motoneuron-evoked synaptic activity was observed in zebrafish embryonic red (ER) and white (EW) muscle fibers paralyzed with a dose of D-tubocurarine insufficient to abolish synaptic activity to determine whether muscle activation was coordinated to produce the undulating body movements required for locomotion. Paired whole-cell recordings revealed a synaptic drive that alternated between ipsilateral and contralateral myotomes and exhibited a rostralcaudal delay in timing appropriate for swimming. Both ER and EW muscle were activated during fictive swimming. However, at the fastest fictive swimming rates, ER fibers were de-recruited, whereas they could be active in isolation of EW fibers at the slowest fictive swimming rates. Prior to hatching, fictive swimming was preceded by a lower frequency, more robust and rhythmic synaptic drive resembling the "coiling" behavior of fish embryos. The motor activity observed in paralyzed zebrafish closely resembled the swimming and coiling behaviors observed in these developing fishes. At the early developmental stages examined in this study, myotomal muscle recruitment and coordination were similar to that observed in adult fishes during swimming. Our results indicate that the patterned activation of myotomal muscle is set from the onset of development.

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Nicotinic acetylcholine receptors (nAChRs) at zebrafish red and white muscle show different properties during development

Ahmed

Ali

2015

Developmental Neurobiology

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Nicotinic acetylcholine receptors (nAChRs) are highly expressed at the vertebrate neuromuscular junction (NMJ) where they are required for muscle activation. Understanding the factors that underlie NMJ development is critical for a full understanding of muscle function. In this study we performed whole cell and outside-out patch clamp recordings, and single-cell RT-qPCR from zebrafish red and white muscle to examine the properties of nAChRs during the first 5 days of development. In red fibers miniature endplate currents (mEPCs) exhibit single exponential time courses at 1.5 days postfertilization (dpf) and double exponential time courses from 2 dpf onwards. In white fibers, mEPCs decay relatively slowly, with a single exponential component at 1.5 dpf. By 2 and 3 dpf, mEPC kinetics speed up, and decay with a double exponential component, and by 4 dpf the exponential decay reverts back to a single component. Single channel recordings confirm the presence of two main conductance classes of nAChRs (∼45 pS and ∼65 pS) in red fibers with multiple time courses. Two main conductance classes are also present in white fibers (∼55 pS and ∼73 pS), but they exhibit shorter mean open times by 5 dpf compared with red muscle. RT-qPCR of mRNA for nicotinic receptor subunits supports a switch from γ to ε subunits in white fibers but not in red. Our findings provide a developmental profile of mEPC properties from red and white fibers in embryonic and larval zebrafish, and reveal previously unknown differences between the NMJs of these muscle fibers.© 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 916-936, 2016.

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Maturation of Neuromuscular Transmission During Early Development in Zebrafish

Cited by 34 publications

References 42 publications

Paired Motor Neuron–Muscle Recordings in Zebrafish Test the Receptor Blockade Model for Shaping Synaptic Current

Paired Motor Neuron–Muscle Recordings in Zebrafish Test the Receptor Blockade Model for Shaping Synaptic Current

Activation of Embryonic Red and White Muscle Fibers During Fictive Swimming in the Developing Zebrafish

Nicotinic acetylcholine receptors (nAChRs) at zebrafish red and white muscle show different properties during development

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