Maturation of neurotransmission in the developing rat cochlea: immunohistochemical evidence from differential expression of synaptophysin and synaptobrevin 2

He, Shan; Yang, Jun

doi:10.4081/ejh.2011.e2

Cited by 9 publications

(9 citation statements)

References 41 publications

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“…In the present study, hair cell-like cells adjacent to SGNs showed presynaptic punctate expression of CtBP2. Expression of SYP, a membrane protein specific to synaptic vesicles, correlates directly with the presence of neurotransmitter [ 49 ]. Expression of SYP was detected at the nerve endings of SGNs adjoining hair cell-like cells.…”

Section: Discussionmentioning

confidence: 99%

Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

et al. 2018

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BackgroundInner ear hair cells as mechanoreceptors are extremely important for hearing. Defects in hair cells are a major cause of deafness. Induced pluripotent stem cells (iPSCs) are promising for regenerating inner ear hair cells and treating hearing loss. Here, we investigated migration, differentiation, and synaptic connections of transplanted otic epithelial progenitors (OEPs) derived from human iPSCs in mouse cochlea.MethodsHuman urinary cells isolated from a healthy donor were reprogramed to form iPSCs that were induced to differentiate into OEPs and hair cell-like cells. Immunocytochemistry, electrophysiological examination, and scanning electron microscopy were used to examine characteristics of induced hair cell-like cells. OEP-derived hair cell-like cells were cocultured with spiral ganglion neurons (SGNs), and the markers of synaptic connections were detected using immunocytochemistry and transmission electron microscope. In vivo, OEPs derived from iPSCs were transplanted into the cochlea of mice by injection through the round window. Migration, differentiation, and synaptic connections of transplanted cells were also examined by thin cochlear sectioning and immunohistochemistry.ResultsThe induced hair cell-like cells displayed typical morphological characteristics and electrophysiological properties specific to inner hair cells. In vitro, OEP-derived hair cell-like cells formed synaptic connections with SGNs in coculture. In vivo, some of the transplanted cells migrated to the site of the resident hair cells in the organ of Corti, differentiated into hair cell-like cells, and formed synaptic connections with native SGNs.ConclusionsWe conclude that the transplantation of OEPs is feasible for the regeneration of hair cells. These results present a substantial reference for a cell-based therapy for the loss of hair cells.

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Section: Discussionmentioning

confidence: 99%

Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

et al. 2018

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“…Immunofluorescence staining was conducted as described previously (45), modified to some extent. Another half of mice in each group were deeply anesthetized with 4% isoflurane.…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model

et al. 2020

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Background: Chronic pain is frequently comorbid with anxiety disorder, thereby complicating its treatment. Polydatin, a component from the root of Polygonum cuspidatum, has shown neuroprotection in the central nervous system. However, its effects on pain and anxiety processing have been rarely investigated. In this study, mice were injected with complete Freund's adjuvant (CFA) at the hindpaw to induce pain-and anxiety-like behaviors. Results: Treatment with polydatin (25 mg/kg) alleviated the anxiety-like behaviors but not pain perception in these mice. Polydatin treatment reversed the upregulation of N-methyl-D-aspartic acid receptors and GluA1-containing a-amino-3hydroxy-5-methyl-4-isoxazole-propionic acid receptors in the amygdala of CFA-injected mice. Additionally, this treatment reduced the levels of proinflammatory cytokines, namely, tumor necrosis factor-alpha and interleukin-1b, in the amygdala. Furthermore, activated nuclear factor kappa-B signaling was blocked in the amygdala from CFA-injected mice. By using docking technology, we found potential structural binding between polydatin and IjB kinase beta. Conclusion: This study indicates the anxiolytic effects of polydatin by suppressing inflammatory cytokines in the amygdala.

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“…The expression of specific protein marker was assessed during pre- and post-natal development in mammalian species, 52 - 65 starting from the process of oocytes maturation and elimination. 55 , 56 , 61 , 62 The development of heart and skeletal muscle was the subject of several papers, where special attention was paid to stem cell populations and their molecular characteristics.…”

Section: Histochemistry and Cytochemistry In Recent Literaturementioning

confidence: 99%

Histochemistry as an irreplaceable approach for investigating functional cytology and histology

Pellicciari

2013

Eur J Histochem

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In agreement with the evolution of histochemistry over the last fifty years and thanks to the impressive advancements in microscopy sciences, the application of cytochemical techniques to light and electron microscopy is more and more addressed to elucidate the functional characteristics of cells and tissue under different physiological, pathological or experimental conditions. Simultaneously, the mere description of composition and morphological features has become increasingly sporadic in the histochemical literature. Since basic research on cell functional organization is essential for understanding the mechanisms responsible for major biological processes such as differentiation or growth control in normal and tumor tissues, histochemical Journals will continue to play a pivotal role in the field of cell and tissue biology in all its structural and functional aspects.

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Maturation of neurotransmission in the developing rat cochlea: immunohistochemical evidence from differential expression of synaptophysin and synaptobrevin 2

Cited by 9 publications

References 41 publications

Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model

Histochemistry as an irreplaceable approach for investigating functional cytology and histology

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