Maturation of the expression of adrenomedullin, endothelin-1 and nitric oxide synthases in adipose tissues from childhood to adulthood

Knerr, Ina; Schirl, C; Horbach, Thomas; Stuppy, Anja; Carbon, R.; Rascher, Wolfgang; Dötsch, Jörg

doi:10.1038/sj.ijo.0802885

Cited by 16 publications

(10 citation statements)

References 31 publications

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“…These results are partly supported by Menghi et al (18), who investigated LEPR expression in meningiomas, and identified that high expression of the LEPR was significantly higher in low-grade meningiomas than high-grade, and it may serve as a relevant prognostic marker to define progression risk in meningioma patients. In support of this finding, Knerr et al (19) observed high LEPR expression in meningiomas.…”

Section: A B C Dsupporting

confidence: 70%

Correlation of leptin receptor expression with BMI in differential grades of human meningiomas

Rutkowski¹,

Reszeć²,

Hermanowicz³

et al. 2016

Oncology Letters

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Abstract. Meningioma is one of the most common primary brain tumor, especially in postmenopausal women. The most important risk factors include radiation, primary head injury or genetic alterations, however it is currently unclear why postmenopausal women are predominantly affected. The aim of the present study was to evaluate leptin receptor (LEPR) expression and body mass index (BMI) in patients with meningiomas of differential grades. Specimens of 158 meningiomas were classified as either G1 (low-grade meningiomas, n=114) or G2/G3 (high-grade meningiomas, n=44). Immunohistochemistry was performed to assess LEPR expression. The mean BMIs of the female and male patient groups were 28.43±5.29 and 23.93±4.66, respectively. Mean BMI was significantly higher in the female group, by ~4.50 kg/m 2 . Patient age significantly correlated with LEPR expression, with the highly positive (++) and positive (+) groups having mean ages of 62.3±12.07 and 52.3±13.04, respectively. A strong positive correlation (r=0.73) was observed between leptin receptor expression and BMI, with the LEPR (++) group having a mean BMI of 30.11±4.49, compared to 22.12±2.48 for the LEPR (+) group. Furthermore, in the low-grade meningioma group, mean BMI was higher in female patients than male patients (28.13±5.54 and 25.38±4.57, respectively; P=0.01). Additionally, there was strong positive correlation between BMI and leptin receptor expression in the low-grade meningioma group (r=0.69). For the high-grade meningioma group, mean BMI was 29.49±4.26 and 21.76±3.98 in female and male patients, respectively, and LEPR expression strongly correlated with BMI in this group (r=0.80). The present study demonstrates a correlation between patient BMI, age, and LEPR expression status in low-and high-grade meningiomas. Our results indicate that in addition to endogenous hormones, such as estrogen or progesterone, or fatty tissue-associated proinflammatory cytokines, LEPR expression status may be a risk factor for meningioma growth and progression.

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Section: A B C Dsupporting

confidence: 70%

Correlation of leptin receptor expression with BMI in differential grades of human meningiomas

Rutkowski¹,

Reszeć²,

Hermanowicz³

et al. 2016

Oncology Letters

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“…10,19,22). In addition, we and others have recently demonstrated that AM is synthesized by human (12,24,29,41) and rodent (9,35) WAT. To the best of our knowledge, there is no report investigating AM and its associated receptor expression and regulation in eWAT.…”

mentioning

confidence: 96%

Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status

Silaghi¹,

Achard²,

Paulmyer‐Lacroix³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

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Epicardial white adipose tissue (eWAT) is in close contact with coronary vessels and therefore could alter coronary homeostasis. Adrenomedullin (AM) is a potent vasodilatator and antioxidative peptide which has been shown to play a cytoprotective role in experimental models of acute myocardial infarction. We studied, using immunohistochemistry and qRT-PCR, the expression of AM and its receptors calcitonin receptor-like receptor (CRLR), and receptor activity-modifying protein (RAMP)2 and -3 in paired biopsies of subcutaneous WAT (sWAT) and eWAT obtained from patients with coronary artery disease (CAD) or without CAD (NCAD). In eWAT obtained from NCAD or CAD patients, immunoreactivity for AM, CRLR, and RAMP2 and -3 was detected in blood vessel walls and isolated stromal cells close to adipocytes. Some of the AM positive stromal cells colocalized CD68 immunoreactivity. eWAT from CAD patients showed increased AM immunoreactivity and AM gene expression. CRLR mRNA levels were comparable in sWAT of both groups and decreased by 40-50% in eWAT, irrespectively of the coronary status. RAMP2 mRNA concentrations did not change while RAMP3 mRNA levels increased in sWAT from CAD patients. There was a positive linear relationship between eWAT 11beta-hydroxysteroid dehydrogenase type 1 mRNA (11beta-HSD-1, the enzyme that converts inactive to active glucocorticoids) and AM mRNA. In conclusion, we demonstrate that AM and its receptors are expressed in eWAT. Our data suggest that eWAT AM, which could originate from macrophages, is related to 11beta-HSD-1 expression. AM synthesis, which is increased in eWAT during chronic CAD in humans, can play a cardioprotective role.

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“…Adipose tissue obtained from rats fed a high fat diet expresses greater amounts of AM than that obtained from rats fed a normal diet (13). Recent studies showed, using real time (RT)-PCR, that AM mRNA is present in human adipose tissue collected during surgery (15,16), and that AM peptide is produced by isolated human adipocytes (14), exposed to an inflammatory environment (16). Identically, human mesenchymal stem cell-derived adipocytes express AM in response to proinflammatory cytokines (16).…”

Section: Introductionmentioning

confidence: 99%

Expression of adrenomedullin in adipose tissue of lean and obese women

et al. 2006

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Objective: Adrenomedullin (AM), a potent vasodilatator and antioxidative peptide, was shown recently to be expressed by adipose tissue. The aim of our study was to investigate the precise localization of AM within human adipose tissue, and to examine AM regulation in obesity. Design: Subcutaneous (SC) and omental (OM) adipose tissues from 9 lean and 13 obese women were profiled for AM expression changes. Preadipocytes from human adipose tissue were isolated and differentiated under defined adipogenic conditions. Methods: AM expression was analyzed by immunohistochemistry, in situ hybridization and quantitative RT-PCR. Results: A strong AM expression was observed in vessel walls, stromal cell clusters and isolated stromal cells, some of them being CD 68 positive, whereas mature adipocytes were not labeled. Calcitonin receptor-like receptor and receptor activity-modifying proteins (RAMP) 2 and RAMP 3 were expressed in vessel walls. In vitro, preadipocytes of early differentiation stages spontaneously secreted AM. No difference in AM localization was found between SC and OM adipose tissue. AM levels in SC tissue did not differ between lean and obese subjects. By contrast, AM levels in OM tissue were significantly higher in obese as compared with lean women. Moreover, we found a positive relationship between OM AM and tumor necrosis factor a mRNA levels and AM-immunoreactive area in OM tissue followed the features of the metabolic syndrome. Conclusion: Stromal cells from human adipose tissue, including macrophages, produce AM. Its synthesis increased in the OM territory during obesity and paralleled the features of the metabolic syndrome. Therefore, AM should be considered as a new member of the adipokine family.

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Maturation of the expression of adrenomedullin, endothelin-1 and nitric oxide synthases in adipose tissues from childhood to adulthood

Cited by 16 publications

References 31 publications

Correlation of leptin receptor expression with BMI in differential grades of human meningiomas

Correlation of leptin receptor expression with BMI in differential grades of human meningiomas

Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status

Expression of adrenomedullin in adipose tissue of lean and obese women

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