Maturation of the head of bacteriophage T4

Laemmli, Ulrich Karl; Favre, Manuel

doi:10.1016/0022-2836(73)90198-8

Cited by 3,674 publications

(756 citation statements)

References 27 publications

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“…10 all our present knowledge about the early pathway of head maturation is summarized. From this and particularly from the observation that the combination of mutant ptE920g (in gene 23, prodcing 40-60% of isometric heads) with conditional, lethal mutant in gene 21 leads t o an increased proportion of isometric 7-particles (36), we conclude that most of the form determination is already expressed at the level of ?.-particles (or prehead I) (37). Only the size increase is left over for one or several of the ensuing steps.…”

Section: General Discussion Of Form Inheritancementioning

confidence: 99%

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

et al. 1974

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In part 1 of this paper ( I ) we give evidence that the P23-capsoid of 7-particles is transformed in situ i n t o the P23*-capsid of' normal phage. Using t h e polymorphism of phage T4, we have chosen polyheads as representative of P 2 3 assemblies and giant phages as representative of P23* assemblies in order t o study their surface crystals by optical filtration of micrographs. We found for polyheads a lattice constant of 1 I 2 a with the typical hexameric, ringlike capsomer and for t h e giants a lattice constant of 124 a with quite a different capsomer morphology, of t h e t y p e (h+l). From the stoichiometry of t h e proteins composing the normal capsid we conclude that the prototner is a single P23* molecule and that the minor capsidproteins must be in singular positions on t h e surface lattice o r on t h e polyhedral head (center of capsomers, vertices, o r basal part).We extrapolate the findings on the giant head to t h e normal head and give a geometric model which is consistent with 1 , I 00 molecules o f P23* per capsid.We discuss t h e part of form inheritance contrihuted by P23 and t h e other formgiving gene products and give evidence that morphologic characters are t h e result of pairs of a reaction chain of interacting gene products. The example we give is the giant head produced by a t s mutant in gene 2 4 at 3h'C.

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Section: General Discussion Of Form Inheritancementioning

confidence: 99%

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

et al. 1974

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“…Samples of digested protein were subjected to polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE) on 10% gels [18], and duplicate samples were then subjected to autoradiography, or electrophoretically transferred to nitrocellulose [19]. After blocking and washing, individual lanes were incubated with human sera at a dilution of between 1 : 100 and 1 : 1000.…”

Section: Sds-page and Imrnunoblottingmentioning

confidence: 99%

Characterisation of the reactivity of autoantibodies in primary biliary cirrhosis

et al. 1989

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Autoantibodies in the sera of patients with primary biliary cirrhosis, shown previously to recognise the E2 polypeptide of the mammalian pyruvate dehydrogenase complex (PDC), have been demonstrated to react with the E2 component of PDC from bacteria (E. coli) and yeast (S. cerevisiae). Limited tryptic digestion, which cleaves E2 into well-characterised domains, followed by Western blotting indicates that the main immunodominant region of PDC E2 lies within the lipoic acid-containing domains of the polypeptide.

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“…With sciatic nerve, deSamples were run on 10% w/v polyacrylamide gels according to the method of Laemmli [5,6]. Alternatively, neurofilaments were irreversibly reduced with NEM before electrophoresis.…”

Section: Preparation Of Rat Nervous System Samplesmentioning

confidence: 99%

In vivo metabolism of mammalian neurofilament polypeptides in developing and adult rat brain

Calvert

Anderton

1982

FEBS Letters

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Maturation of the head of bacteriophage T4

Cited by 3,674 publications

References 27 publications

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

The transformation of τ particles into t4 heads. II. Transformations of the surface lattice an related observations on form determination

Characterisation of the reactivity of autoantibodies in primary biliary cirrhosis

In vivo metabolism of mammalian neurofilament polypeptides in developing and adult rat brain

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