Maturation rates of oocytes and levels of FSHR, LHR and GnRHR of COCs response to FSH concentrations in IVM media for sheep

Wei, Shaozhong; Gong, Zhuandı; Sheng, Li; Haoqin, Liang; Lai, Liangxue; Deng, Yingying

doi:10.1016/j.jab.2017.01.001

Cited by 9 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In cumulus cells, the expression levels of FSHR, EGFR and GHR mRNA increased with follicular size [3]. FSH can accelerate the maturation rate of sheep oocytes and reduce the rate of apoptosis, but also increase the [21]. Variable expression are observed in almost all of the genes in the present study at high and low fecundity, and the FSHR record was the most significant for the high and low fecundity of the ovarian follicles of Indian sheep ewes [5].…”

Section: Discussionmentioning

confidence: 43%

“…FSHR has significance regarding the modulation of animal reproductive functions [10,23]. Moreover, previous studies found that the FSH could accelerate the maturation rate of sheep oocytes and reduce their apoptosis rate, increase the expression levels of FSHR, LHR and gonadotropin releasing hormone receptor (GnRHR) mRNAs, and strengthen expressions of FSHR and GnRHR proteins [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Chen¹,

Cui²,

Yu³

2018

Folia Morphol

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Chen¹,

Cui²,

Yu³

2018

Folia Morphol

View full text Add to dashboard Cite

show abstract

“…Follicle stimulating hormone and LH bind to specific receptors in oocytes, activate intracellular steroidogenic signaling pathway and negatively regulate FSH and LH, thus induced the proliferation and differentiation of ovarian GCs [ 43 , 44 , 45 ]. The role of GnRHR in ovarian development is mainly to promote the secretion of FSH and LH through the activity of GnRH [ 46 ]. Wei et al [ 24 ] reported that Alarelin immunization could stimulate the production of GnRH antibody, inhibit the expression of GnRHR protein, enhance the expressions of FSHR and LHR protein in the ovary, and increase the secretion of FSH, so as to regulate the development of ovary and follicle in ewes.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts

Wan

Yuan

Yang

et al. 2021

Toxins

View full text Add to dashboard Cite

This study aims to investigate the effects of zearalenone (ZEA) on the localizations and expressions of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), gonadotropin releasing hormone (GnRH) and gonadotropin releasing hormone receptor (GnRHR) in the ovaries of weaned gilts. Twenty 42-day-old weaned gilts were randomly allocated into two groups, and treated with a control diet and a ZEA-contaminated diet (ZEA 1.04 mg/kg), respectively. After 7-day adjustment, gilts were fed individually for 35 days and euthanized for blood and ovarian samples collection before morning feeding on the 36th day. Serum hormones of E2, PRG, FSH, LH and GnRH were determined using radioimmunoassay kits. The ovaries were collected for relative mRNA and protein expression, and immunohistochemical analysis of FSHR, LHR, GnRH and GnRHR. The results revealed that ZEA exposure significantly increased the final vulva area (p < 0.05), significantly elevated the serum concentrations of estradiol, follicle stimulating hormone and GnRH (p < 0.05), and markedly up-regulated the mRNA and protein expressions of FSHR, LHR, GnRH and GnRHR (p < 0.05). Besides, the results of immunohistochemistry showed that the immunoreactive substances of ovarian FSHR, LHR, GnRH and GnRHR in the gilts fed the ZEA-contaminated diet were stronger than the gilts fed the control diet. Our findings indicated that dietary ZEA (1.04 mg/kg) could cause follicular proliferation by interfering with the localization and expression of FSHR, LHR, GnRH and GnRHR, and then affect the follicular development of weaned gilts.

show abstract

“…However, the same concentration of FSH, 0.5μg.mL -1 , has resulted in lower rates of maturation in llamas (Del Campo et al 1994). Similarly, in ewes the rate of oocyte maturation varies amongst different concentrations of FSH (Wei et al 2017;Wei et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies in alpacas used 0.5µg.mL -1 of FSH as a supplement in maturation medium at a fixed concentration during in vitro maturation of oocytes (Ratto et al 2007;Huanca et al 2014;Ruiz et al 2017), in this process, the use of eCG in alpacas is not typical. However, in other species, different concentrations of gonadotropins in maturation medium have been studied (Choi et al 2001;Farag et al 2012;Wei et al 2017;Wei et al 2018). In dromedary camels, a high in vitro maturation rate of oocytes was reported when supplemented with 10µg.mL -1 of pregnant mare serum gonadotropin (PMSG) plus human chorionic gonadotropin (Farag et al 2012;Farag et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of FSH and eCG on Alpaca (Vicugna pacos) Oocyte Maturation in vitro

2021

Int J Vet Sci

View full text Add to dashboard Cite

This study evaluated alpaca oocytes that developed to metaphase II (MII), using different concentrations of follicle stimulating hormone (FSH) with and without equine chorionic gonadotropin (eCG) during in vitro maturation. Oocytes were obtained from ovaries of slaughtered alpacas. Oocytes were matured in vitro for 36h in TCM-99 and supplemented in groups with different doses of FSH: 0.5, 0.25, 0μg.mL-1 and eCG: 15, 5, 0IU.mL-1. Oocytes were stained with 2% lacmoid, and examined for their nuclear status. The parameter for comparisons between groups was the percentage of oocytes in MII. There was an interaction between FSH and eCG (P<0.05). A higher percentage of oocytes in MII were obtained when using 0.5µg.mL-1 of FSH with any concentration of 15, 5 or 0IU.mL-1 of eCG [58.4±1.94% (n=78); 59.5±1.94% (n=85); 54.3±0.56% (n=81); P<0.05]. A similar percentage was also found using 0.25µg.mL-1 of FSH plus 15IU.mL-1 of eCG [52.3±1.66% (n=86); P>0.05] followed by 0.25µg.mL-1 of FSH with 5 or 0IU.mL-1 of eCG [47.3±1.94% (n=82); 45.3±1.94% (n=86); P<0.05]. The lowest maturation percentages were found using any concentration of eCG without FSH [8.2±0.73% (n=84); 8.0±0.66% (n=74); 11.4±1.23% (n=71); P<0.05]. In conclusion, the addition of eCG to the maturation medium would reduce the amount of FSH required in the in vitro maturation of alpaca oocytes.

show abstract

Maturation rates of oocytes and levels of FSHR, LHR and GnRHR of COCs response to FSH concentrations in IVM media for sheep

Cited by 9 publications

References 20 publications

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts

Effect of FSH and eCG on Alpaca (Vicugna pacos) Oocyte Maturation in vitro

Contact Info

Product

Resources

About