Maturation rates of vitrified-thawed immature buffalo (Bubalus bubalis) oocytes: effect of different types of cryoprotectants

Wani, Nisar Ahmad; Misra, A. K.; Maurya, S. N.

doi:10.1016/j.anireprosci.2004.02.007

Cited by 23 publications

(22 citation statements)

References 20 publications

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Section: Introductionmentioning

confidence: 99%

“…Bovine oocytes were also vitrified and remained viable for offspring production after in vitro fertilization and embryo transplantation [17,18]. vitrified buffalo oocytes with 51.1% glycerol via the straw method, obtaining a maturation rate of 23.5% after thawing [18]. When glycerol was used with EG, which increased permeability of the cell membrane during oocyte vitrification, and maturation rates of 30 s exposure groups did not differ from those of controls [19].…”

Section: Introductionmentioning

confidence: 99%

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An efficient method for the sanitary vitrification of bovine oocytes in straws

Zhou

et al. 2014

J Animal Sci Biotechnol

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BackgroundAt present, vitrification has been widely applied to humans, mice and farm animals. To improve the efficiency of vitrification in straw, bovine oocytes were used to test a new two-step vitrification method in this study.ResultsWhen in vitro matured oocytes were exposed to 20% ethylene glycol (EG20) for 5 min and 40% ethylene glycol (EG40) for 30 s, followed by treatment with 30% glycerol (Gly30), Gly40 or Gly50, a volume expansion was observed in Gly30 and Gly40 but not Gly50. This indicates that the intracellular osmotic pressure after a 30 s differs between EG40 and ranged between Gly40 (approximately 5.6 mol/L) and Gly50 (approximately 7.0 mol/L). Since oocytes are in EG40 just for only a short period of time (30 s) and at a lower temperature (4°C), we hypothesize that the main function of this step in to induce dehydration. Based on these results, we omitted the EG40 step, before oocytes were pretreated in EG20 for 5 min, exposed to pre-cooled (4°C) Gly50, for 30 s, and then dipped into liquid nitrogen. After warming, 81.1% of the oocytes survived, and the surviving oocytes developed into cleavage stage embryos (63.5%) or blastocysts (20.0%) after parthenogenetic activation.ConclusionsThese results demonstrate that in a two-step vitrification procedure, the permeability effect in the second step is not necessary. It is possible that the second step is only required to provide adequate osmotic pressure to condense the intracellular concentration of CPAs to a level required for successful vitrification.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An efficient method for the sanitary vitrification of bovine oocytes in straws

Zhou

et al. 2014

J Animal Sci Biotechnol

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“…The oocytes were separated at the end of the maturation and stained by aceto‐orcein stain to evaluate nuclear maturation as described before (Wani, Misra, et al, ). Orcein is used in form of a 1% solution in 45% acetic acid.…”

Section: Methodsmentioning

confidence: 99%

“…Optimum utilization of slaughterhouse material and development of an efficient oocytes maturation system that takes into account all the factors essential for the completion of oocytes maturation in vitro are very essential for this species (Wani & Wernery, ). Cryopreservation of oocytes collected from slaughtered animals of high genetic value and their subsequent application for production of transferable embryos may provide a chance to overcome the valuable germplasm lost and enable the genetic improvement of the species (Wani, Misra, & Maurya, ). Cryopreservations of germplasm were performed either by slow freezing (Gautam, Verma, Palta, Chauhan, & Manik, ) or vitrification (Yamada et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of cryoprotectants and cryoprotectant combinations on viability and maturation rates of Camelus dromedarius oocytes vitrified at germinal vesicle stage

Moawad

Hussein

El-Ghani

et al. 2018

Reprod Domestic Animals

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Camel fertility faces many problems, which could be solved by assisted reproductive technologies (ARTs). We designed the experiment to explore the effect of different cryoprotectant concentrations and combinations on viability and maturation rates of vitrified/warmed camel oocytes. We collected ovaries directly after slaughtering from local abattoir and transported them to laboratory in a thermo-flask containing normal physiological saline. We aspirated the oocytes from follicles, which is 2-8 mm in diameter, washed three times in TCM-199 and then examined under stereo-microscope for selection. We selected morphologically normal oocytes with an evenly granulated cytoplasm and a compact cumulus cell layer. We equilibrated morphologically normal oocytes in equilibration solution (ES), which is half concentration of vitrification one. After equilibration, We transported oocytes to vitrification solution using ethylene glycol (EG, 40%), dimethyl sulphoxide (DMSO, 40%) and EG 40% + DMSO 40%. The obtained results revealed that addition of EG 40% + DMSO 40% resulted in the best quality of vitrified/warmed oocytes, which is demonstrated by higher per cent survival rate (90.16%) and maturation rate (58.95%). While DMSO 40% resulted in 62.79% and 29.54%, respectively, EG 40% reported 86.11% and 53.47%, respectively. We could conclude that vitrification of immature camel oocytes by using 40% EG + 40% DMSO is suitable methods to limit drawbacks of vitrification methods, and we need further studies to assess the ability of in vitro-produced blastocyst to develop in vivo and establish pregnancy after embryo transfer.

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“…There are few studies about oocyte vitrification using GLY alone. Buffalo oocytes vitrified with 51.1% GLY in the absence of other cryoprotectants yield 23.5% maturation rate after thawing (Wani et al. 2004).…”

Section: What Aspects Can Be Considered For Cryopreserving Bovine Oocmentioning

confidence: 99%

Cryopreservation of the Bovine Oocyte: Current Status and Perspectives

Díez

Muñoz

Caamaño

et al. 2012

Reprod Domestic Animals

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ContentsThis review presents some of the most noticeable aspects related with the oocyte cryopreservation procedures, emphasizing their evolution in the bovine, which points towards the critical points determining the reduced survival rates of female gametes to freezing and vitrification. Factors such as the maturation status, the cytoskeleton and membrane sensitivity, the role of the cumulus cells, the impact of the cryoprotectants agents and the protocols utilized and the future of this tool have been extensively reviewed.

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Maturation rates of vitrified-thawed immature buffalo (Bubalus bubalis) oocytes: effect of different types of cryoprotectants

Cited by 23 publications

References 20 publications

An efficient method for the sanitary vitrification of bovine oocytes in straws

An efficient method for the sanitary vitrification of bovine oocytes in straws

Effects of cryoprotectants and cryoprotectant combinations on viability and maturation rates of Camelus dromedarius oocytes vitrified at germinal vesicle stage

Cryopreservation of the Bovine Oocyte: Current Status and Perspectives

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