Maturational changes of the CD52-like epididymal glycoprotein on cynomolgus monkey sperm and their apparent reversal in capacitation conditions

Yeung, Ching‐Hei; Schröter, Sabine; Kirchhoff, Christiane; Cooper, Trevor G.

doi:10.1002/1098-2795(200011)57:3<280::aid-mrd10>3.0.co;2-5

Cited by 23 publications

(13 citation statements)

References 45 publications

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“…DEFB126 is a b-defensin which appears to be unique among sperm surface coating proteins, in that it uniformly spans the entire sperm surface and is not exclusive to a specific domain [23]. CD52 has also been reported to coat the whole sperm, but there is an elevated presence over the equatorial segment and variable labeling depending on where the sperm resided in the male reproductive tract and the state of capacitation [14,24,25]. The question of whether the uniform distribution of 1 DEFB126 on the macaque sperm surface can serve as an immunoprotective shield was initially proposed by Tollner et al [20] and is the focus of this study.…”

Section: Introductionmentioning

confidence: 58%

Beta-Defensin 126 on the Cell Surface Protects Sperm from Immunorecognition and Binding of Anti-Sperm Antibodies1

Yudin

Generao

Tollner

et al. 2005

Biology of Reproduction

119

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Beta-defensin 126 (DEFB126), formerly known as epididymal secretory protein 13.2 (ESP13.2), coats the entire primate sperm surface until completion of capacitation, and it is a candidate for providing immune protection in the female reproductive tract. To further examine the potential role of DEFB126 as a means of protection from immune recognition, cynomolgus macaque sperm were exposed to a number of treatments that are known to alter sperm surface coats, including capacitation. We used a novel in vivo assay to determine immune recognition: aldehyde-fixed whole sperm injections into rabbits. Following booster injections, immunoblot analyses of whole sperm prepared in various manners was conducted. On Days 60 and 80 post-initial immunization, the antisera showed a remarkably strong reaction to a single 34-36 kDa protein, which was shown to be DEFB126. Sera from rabbits that were immunized with sperm washed more rigorously using Percoll gradients showed an increase in the number and intensity of proteins recognized on whole sperm Western blots, although DEFB126 was still the major immune response. When capacitated sperm, from which most DEFB126 had been released, were used as the immunogen, there was a dramatic increase in the immune recognition to a variety of protein bands. Sperm treated with neuraminidase to remove sialic acid on DEFB126 before fixation were shown to still possess DEFB126, but lacked the sialic acid component of the glycoprotein. These sperm were as immunogenic as capacitated sperm even though the desialylated DEFB126 still covered the entire cell surface. These sperm lost their highly negative charge (the isoelectric point of DEFB126 shifted from pI 3.0 to pI 6.4). Experiments using different sperm plasma membrane protein-specific Igs showed that recognition did not occur when DEFB126 was present, but following capacitation these Igs readily recognized the exposed sperm membrane. Our data suggest that DEFB126 protects the entire primate sperm surface from immune recognition and that the sialic acid moieties are responsible for the cloaking characteristic of this unique glycoprotein.

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Section: Introductionmentioning

confidence: 58%

Beta-Defensin 126 on the Cell Surface Protects Sperm from Immunorecognition and Binding of Anti-Sperm Antibodies1

Yudin

Generao

Tollner

et al. 2005

Biology of Reproduction

119

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“…However, although macromolecules such as 'HIS protein' and 'CD52' have been designated as 'maturation associated', most spermatozoa develop the ability to fertilize before such macromolecules are acquired. Therefore, it seems likely that such molecules act rather to facilitate the storage of the spermatozoa and, as argued below, they may underlie or at least contribute to the need for capacitation in the female reproductive tract (see Yeung et al (2000)). …”

Section: Sperm Storage In the Cauda Epididymidismentioning

confidence: 99%

Singular features of fertilization and their impact on the male reproductive system in eutherian mammals

Bedford

2014

Reproduction

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Therian (marsupial and eutherian) mammals have evolved a suite of novel reproductive features -seen variously in their gametes, the steps of fertilization and the male reproductive tract -whose adaptive significance remains unclear. Present evidence for the betterunderstood eutherian mammals suggests that the 'prime mover' in their evolution has been the character of the egg coat, with other such features being adaptations to the consequences of this. Its elastic thickness allows the zona pellucida to stretch to a variable degree and yet remain around the blastocyst during much or all of its expansion before implantation, but its character represents an unusual challenge for spermatozoa. Novel aspects of the acrosome related to this challenge enable it to maintain a relatively prolonged binding after the onset of the acrosome reaction, and the structure, shape and behaviour of the sperm head point to physical thrust as a major element of zona penetration -with the unique configuration of gamete fusion as a sequela of this strategy. In the male, such adaptations are reflected in sperm head formation in the testis and in sperm maturation in the epididymis involving at least the sperm head's structure, plasmalemma and acrosome. This complexity allied to a slow epididymal sperm transport, a relatively modest sperm production and the brief life span of mature spermatozoa kept above the cauda epididymidis could account for the evolution of the sperm storage function -a development seemingly linked, in turn, to the need for sperm capacitation and scrotal evolution.Reproduction (2014) 147 R43-R52

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“…Nonetheless, the molecular nature of the capacitated-related change(s) in the sperm plasma membrane has proven hard to pin down, and probably differs somewhat among different mammals. There is an increasing consensus, however, that in some species including man capacitation involves a loss or redistribution over both the sperm head and tail surfaces of molecules acquired in the lower corpus/upper cauda regions of the epididymis that may act to prolong the life of sperm there -specifically sperm membrane cholesterol (Cross, 1998;Osheroff et al, 1999), and of certain glycoproteins (Yeung et al, 2000). In general accord with this concept, mature porcine spermatozoa from the upper epididymis developed the ability to fertilize in the oviduct sooner than those from the lower cauda (Hunter, Holtz and Henfrey, 1976), whereas capacitation was prolonged if they were first exposed to cauda secretions (Hunter, Holtz and Hermann, 1978).…”

Section: Sperm Capacitationmentioning

confidence: 99%

Puzzles of mammalian fertilization and beyond

Bedford¹

2008

Int. J. Dev. Biol.

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A complexity imposed on the events of conception during the evolution of eutherian mammals is reflected not only in the placenta but also in a series of gamete-related novelties that involve the design of sperm and eggs, their interactions leading to fertilization, epididymal function, and even the scrotal state. While their functional significance has been difficult to determine, I suggest on the basis of present evidence that the genesis of these novel features relates ultimately to changes in the egg associated with nidation, that they imply a new fertilization strategy, and that most are causally linked -a major first 'domino' being the formidable zona pellucida. The eutherian zona pellucida stands out by virtue of an elastic resilience and thickness which allow it to stretch around the expanding blastocyst. However, this quality of the zona together with its relative protease-insensitivity creates a physical challenge which appears to have determined the design of the sperm head, its behaviour at fertilization, and indirectly even the pattern of sperm maturation in the epididymis. In turn, that pattern appears to have evoked the development of the sperm storage function of the cauda region, with both sperm capacitation and the scrotal state as ultimate legacies of this. Coincidentally, the vulnerability of the small yolkless egg to polyspermy appears to have selected for a unique parsimonious pattern of sperm transport in the Fallopian tube, and possibly for the role of the cumulus oophorus as a sperm sequestering device. These ideas are supported generally by evidence from marsupials, as well as by the deviant patterns seen in some insectivorous mammals.

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Maturational changes of the CD52-like epididymal glycoprotein on cynomolgus monkey sperm and their apparent reversal in capacitation conditions

Cited by 23 publications

References 45 publications

Beta-Defensin 126 on the Cell Surface Protects Sperm from Immunorecognition and Binding of Anti-Sperm Antibodies1

Beta-Defensin 126 on the Cell Surface Protects Sperm from Immunorecognition and Binding of Anti-Sperm Antibodies1

Singular features of fertilization and their impact on the male reproductive system in eutherian mammals

Puzzles of mammalian fertilization and beyond

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