Maximization of STR DNA typing success for touched objects

Prinz, Mechthild; Schiffner, Linnea A; Sebestyen, James; Bajda, Ewelina; Tamariz, Jeannie; Shaler, Robert C.; Baum, Howard; Caragine, Theresa

doi:10.1016/j.ics.2005.10.051

Cited by 24 publications

(10 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fingerprints can be an important, but potentially unreliable source of DNA for forensic STR testing. Fingerprints can yield little to no DNA or they can result in DNA profiles that are suitable for upload to forensic STR databases . Using fingerprints in forensic science would include collecting potential evidence from crime scenes, such as the handles of weapons, tools used in criminal cases, and clothing with no apparent staining.…”

Section: Discussionmentioning

confidence: 99%

Qualitative and quantitative assessment of single fingerprints in forensic DNA analysis

et al. 2014

View full text Add to dashboard Cite

Fingerprints and touched items are important sources of DNA for STR profiling, since this evidence can be recovered in a wide variety of criminal offenses. However, there are some fundamental difficulties in working with these samples, including variability in quantity and quality of extracted DNA. In this study, we collected and analyzed over 700 fingerprints. We compared a commercially available extraction protocol (Zygem) to two methods developed in our laboratory, a simple one-tube protocol and a high sensitivity protocol (HighSens) that includes additional steps to concentrate and purify the DNA. The amplification protocols tested were AmpFLSTR® Identifiler® using either 28 or 31 amplification cycles, and Identifiler® Plus using 32 amplification cycles. We found that the HighSens and Zygem extraction methods were significantly better in their DNA yields than the one-tube method. Identifiler® Plus increased the quality of the STR profiles for the one-tube extraction significantly. However, this effect could not be verified for the other extraction methods. Furthermore, microscopic analysis of single fingerprints revealed that some individuals tended to shed more material than others onto glass slides. However, a dense deposition of skin flakes did not strongly correlate with a high quality STR profile.

show abstract

Section: Discussionmentioning

confidence: 99%

Qualitative and quantitative assessment of single fingerprints in forensic DNA analysis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Moreover, peak heights for each color, injection parameter, and instrument were consistent. Furthermore, 109 non-probative casework samples from touched or handled items or single fingerprints were also examined (30). At least 40 pg of DNA was recovered from 42% of these samples, with four containing sufficient DNA for HT-DNA testing.…”

Section: Stochastic Effects Spurious Alíeles and Stuttermentioning

confidence: 99%

Validation of Testing and Interpretation Protocols for Low Template DNA Samples Using AmpFℓSTR® Identifiler®

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…and several other researchers, showed that a DNA profile could be generated from fingerprints, and, as a result, collection, and storage methods for fingerprints have changed over time . When DNA analysis is the only desired examination, fingerprints will often be swabbed directly ( in situ ) in place of the archiving method described above . Nonetheless, archived latent fingerprints continue to exist as evidence from many older cases; these specific samples could therefore be a valuable source of DNA evidence even after numerous years of storage.…”

mentioning

confidence: 99%

An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints

Solomon

Hytinen

McClain

et al. 2017

Journal of Forensic Sciences

View full text Add to dashboard Cite

DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints-touch DNA "sandwiched" between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post-amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAampâ DNA Investigator Kit, and concentration with Centri-Sep TM columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7-100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases.

show abstract

Maximization of STR DNA typing success for touched objects

Cited by 24 publications

References 2 publications

Qualitative and quantitative assessment of single fingerprints in forensic DNA analysis

Qualitative and quantitative assessment of single fingerprints in forensic DNA analysis

Validation of Testing and Interpretation Protocols for Low Template DNA Samples Using AmpFℓSTR® Identifiler®

An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints

Contact Info

Product

Resources

About