2015
DOI: 10.1039/c5ra18673k
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Maximizing fibroblast adhesion on protein-coated surfaces using microfluidic cell printing

Abstract: translation of in vitro cell based assays to in vivo cellular response is imprecise at best. The advent of three-dimensional cell cultures in addition to bioreactor type microfluidics has improved the situation. However, these technical advances cannot be easily combined due to practical limitations. Development of a vertical microfluidic cell printer overcomes this obstacle, providing the ability to more closely recapitulate complex cellular environments and responses. As a proof of concept, we investigated t… Show more

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Cited by 4 publications
(2 citation statements)
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“…Therefore, if the cell culture can survive the highest concentration, then the cell growth density would even increase with decreasing SWCNT concentration in the composite. The fluorescence images ( Figure 5 a−h) show exemplary images of living fibroblasts cultured directly on the SWCNT-based nanocomposites within 24 and 48 h. For the 24 h reference ( Figure 5 d) samples, cell densities of ~90 mm −2 are obtained, which is comparable to standard values for fibroblast-coated substrates [ 57 ]. For 24 h albumin and acrylic polymer-based samples ( Figure 5 a,c), the density of cells increases strongly (~210 mm −2 ) in comparison to the reference.…”
Section: Resultsmentioning
confidence: 54%
“…Therefore, if the cell culture can survive the highest concentration, then the cell growth density would even increase with decreasing SWCNT concentration in the composite. The fluorescence images ( Figure 5 a−h) show exemplary images of living fibroblasts cultured directly on the SWCNT-based nanocomposites within 24 and 48 h. For the 24 h reference ( Figure 5 d) samples, cell densities of ~90 mm −2 are obtained, which is comparable to standard values for fibroblast-coated substrates [ 57 ]. For 24 h albumin and acrylic polymer-based samples ( Figure 5 a,c), the density of cells increases strongly (~210 mm −2 ) in comparison to the reference.…”
Section: Resultsmentioning
confidence: 54%
“…Hence, they hold great applications in drug discovery chips, cell staining devices [9] and microbioreactors [10]. For cell immobilization, there are several approaches such as encapsulation within photo cross-linkable polymers [11], adhesion to patterned proteins [12] and protein coatings [13].…”
Section: Introductionmentioning
confidence: 99%