Maximizing the reliability of non-invasive endocrine sampling in the tiger (<i>Panthera tigris</i>): environmental decay and intra-sample variation in faecal glucocorticoid metabolites

Parnell, Tempe Isobel; Narayan, Edward; Nicolson, V.; Martin-Vegue, Patrick; Mucci, Al; Hero, Jean‐Marc

doi:10.1093/conphys/cov053

Cited by 15 publications

(9 citation statements)

References 34 publications

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“…Our results indicate that glucocorticoid metabolite measurements from fecal pellets may remain stable when deposited into an environment with persistent sub-freezing temperatures and no snowmelt. This is in contrast to regions with more moderate climate conditions because both gut microbes and environmental microbes degrade fecal masses, beginning at deposition (Möstl et al, 1999; Parnell et al 2015) and their activity may be accelerated or inhibited by ambient conditions (Mesa-Cruz et al, 2014). In warm, wet climates, fecal samples are likely to degrade in less than a day, as a consequence of high microbial and enzymatic activity.…”

Section: Discussionmentioning

confidence: 98%

Does persistent snowpack inhibit degradation of fecal stress indicators?

Parikh

Webster

Vucetich

et al. 2018

Conservation Physiology

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Section: Discussionmentioning

confidence: 98%

Does persistent snowpack inhibit degradation of fecal stress indicators?

Parikh

Webster

Vucetich

et al. 2018

Conservation Physiology

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“…For example, fecal samples maintained for 5-hours at room temperatures had similar levels as samples immediately frozen following collection (Dantzer et al, 2010). At ambient temperature, metabolites are reported to be stable for up to 48-h (Parnell et al, 2015). Soiled traps were cleaned with 70% ethanol between uses to ensure that the feces collected from each trap belonged to the animal caught in the trap that night.…”

Section: Small Mammal Trappingmentioning

confidence: 99%

No island-effect on glucocorticoid levels for a rodent from a near-shore archipelago

Stewart

Mastromonaco

Burness

2020

PeerJ

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Island rodents are often larger and live at higher population densities than their mainland counterparts, characteristics that have been referred to as ''island syndrome''. Island syndrome has been well studied, but few studies have tested for island-mainland differences in stress physiology. We evaluated island syndrome within the context of stress physiology of white-footed mice (Peromyscus leucopus) captured from 11 islands and five mainland sites in Thousand Islands National Park, Ontario, Canada. Stress physiology was evaluated by quantifying corticosterone (a stress biomarker), the primary glucocorticoid in mice, from hair and its related metabolites from fecal samples. White-footed mice captured in this near-shore archipelago did not display characteristics of island syndrome, nor differences in levels of hair corticosterone or fecal corticosterone metabolites compared with mainland mice. We suggest that island white-footed mice experience similar degrees of stress in the Thousand Islands compared with the mainland. Although we did not find evidence of island syndrome or differences in glucocorticoid levels, we identified relationships between internal (sex, body mass) and external (season) factors and our hormonal indices of stress in whitefooted mice. . ArensA, McNab BK. 2001. The comparative energetics of caviomorph rodents. Comparative Biochemistry and Physiology Part A 130:105-122. Barrett K, Anderson WB, Wait DA, Grismer LL, Polis GA, Rose MD. 2005. Marine subsidies alter the diet and abundance of insular and coastal lizard populations. Dewsbury DA, Baumgardner DJ, Evans RL, Webster DG. 1980. Sexual dimorphism for body mass in 13 taxa of muroid rodents under laboratory conditions. Journal of Mammalogy 61:146-149 DOI 10.2307/1379975. Erickson RL, Browne CA, Lucki I. 2017. Hair corticsterone measurement in mouse models of type 1 and type 2 diabetes mellitus. Physiology and Behavior 178:166-171 hormone research in uncontrolled, natural environments: the problem with sex, diet, metabolic rate and the individual: non-invasive hormone research. Methods in Ecology and Evolution 3:757-765 Harper GA, Rutherford M. 2016. Home range and population density of black rats (Rattus rattus) on a seabird island: a case for a marine subsidised effect? New Zealand Journal of Ecology 40:219-228. Harper JM, Austad SN. 2000. Fecal glucocorticoids: a noninvasive method of measuring adrenal activity in wild and captive rodents. Physiological and Biochemical Zoology: Ecological and Evolutionary Approaches 73:12-22 DOI 10.1086/316721. Harper JM, Austad SN. 2001. Effect of capture and season on fecal glucocorticoid levels in deer mice (peromyscus maniculatus) and red-backed voles (clethrionomys gapperi). General and Comparative Endocrinology 123:337-344 DOI 10.1006/gcen.2001.7682. Hayssen V, Harper JM, DeFina R. 2002. Fecal corticosteroids in agouti and non-agouti deer mice (Peromyscus maniculatus). Comparative Biochemistry and Physiology Part . Holding ML, Frazier JA, Dorr SW, Pollock NB, Muelleman PJ, Branske A, Henningsen SN...

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“…For example, fecal samples maintained for 5-hours at room temperatures had similar levels as samples immediately frozen following collection (Dantzer et al, 2010). At ambient temperature, metabolites are reported to be stable for up to 48-hrs (Parnell et al, 2015). Soiled traps were cleaned with 70% ethanol between uses to ensure that the feces collected from each trap belonged to the animal caught in the trap that night.…”

Section: Small Mammal Trappingmentioning

confidence: 99%

Peer Review #2 of "No island-effect on glucocorticoid levels for a rodent from a near-shore archipelago (v0.1)"

2020

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Island rodents are often larger and live at higher population densities than their mainland counterparts, characteristics that have been referred to as "island syndrome". Island syndrome has been well studied, but few studies have tested for island-mainland differences in stress physiology. We evaluated island syndrome within the context of stress physiology of white-footed mice (Peromyscus leucopus) captured from 11 islands and 5 mainland sites in Thousand Islands National Park, Ontario, Canada. Stress physiology was evaluated by quantifying corticosterone (a stress biomarker), the primary glucocorticoid in mice, from hair and its related metabolites from fecal samples. White-footed mice captured in this near-shore archipelago did not display characteristics of island syndrome, nor differences in levels of hair corticosterone or fecal corticosterone metabolites compared with mainland mice. We suggest that island white-footed mice experience similar degrees of stress in the Thousand Islands compared with the mainland. Although we did not find evidence of island syndrome or differences in glucocorticoid levels, we identified relationships between internal (sex, body mass) and external (season) factors and our hormonal indices of stress in white-footed mice.

show abstract

Maximizing the reliability of non-invasive endocrine sampling in the tiger (Panthera tigris): environmental decay and intra-sample variation in faecal glucocorticoid metabolites

Cited by 15 publications

References 34 publications

Does persistent snowpack inhibit degradation of fecal stress indicators?

Does persistent snowpack inhibit degradation of fecal stress indicators?

No island-effect on glucocorticoid levels for a rodent from a near-shore archipelago

Peer Review #2 of "No island-effect on glucocorticoid levels for a rodent from a near-shore archipelago (v0.1)"

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