Maximizing the safety of glycerol preserved human amniotic membrane as a biological dressing

Zidan, Serag M.; Eleowa, Samy; Nasef, Mahmoud; Abd-Almoktader, Magdy A.; Elbatawy, Amr; Borhamy, Ahmad G.; Aboliela, Mohammad A.; Ali, Ahmad M.; Algamal, Mahmoud R.

doi:10.1016/j.burns.2015.03.009

Cited by 25 publications

(32 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to the outcomes mentioned earlier, the findings of this study reported many secondary outcomes that proved HAM to be more effective than routine dressings. The HAM group experienced less pain upon removal of dressings and less discharge from the donor site compared with the non‐HAM group . Similarly, the former group was associated with less pruritis, dressing changes, and discomfort than the latter group …”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

Human Amniotic Membrane: A New Option for Graft Donor Sites – Systematic Review and Meta‐analysis

Abul

Karam

Rahman

2020

International Wound Journal

View full text Add to dashboard Cite

There are currently no standardised guidelines on the optimum dressing used for graft donor sites. The aim was to compare the outcomes of human amniotic membrane (HAM) vs routine dressings in split‐thickness skin graft (STSG) donor site healing. A systematic review and meta‐analysis was performed and a search of electronic information was conducted to identify all randomised controlled trials comparing the outcomes of HAM vs routine dressings in STSG donor sites. Wound healing and infection rate were primary outcome measures. Secondary outcome measures included severity of pain, discharge from donor site, the number of dressing changes, pruritus, and comfort. Fixed effect modelling was used for the analysis. Four studies enrolling 157 patients were identified. There was a significant difference between HAM and routine groups with wound healing time (P < .0001) and proportion of wounds healed by day 12 (P = .01). There was no significant difference between the two groups in infection rates (P = .27). For all secondary outcomes, HAM had improved results. HAM dressings are a superior option when compared with routine dressings used in current clinical practice for STSG donor sites as they improve wound healing and do not increase the infection rate.

show abstract

Section: Discussionmentioning

confidence: 88%

“…We assessed wound healing in terms of the time taken as well as the proportion of wounds healed by day 12. Salehi et al and Zidan et al reported wound healing times whereas Loefebellian et al and Singh et al reported the proportion of wounds healed by day 12.…”

Section: Resultsmentioning

confidence: 99%

Human Amniotic Membrane: A New Option for Graft Donor Sites – Systematic Review and Meta‐analysis

Abul

Karam

Rahman

2020

International Wound Journal

View full text Add to dashboard Cite

show abstract

“…The decellularization efficiency and the biocompatibility as an implantable biological mesh after treatment were verified [11]. The decellularized HAM contains only basement layer with the components of collagen types I, III and IV, laminin (LN), fibronectin and a variety of growth factors [12,13]. The LN contains a variety of protein molecules, which composes extracellular matrix (ECM) as non-collagen glycoprotein.…”

Section: Introductionmentioning

confidence: 99%

The repairing of full-thickness skin deficiency and its biological mechanism using decellularized human amniotic membrane as the wound dressing

Song

Wang

et al. 2017

Materials Science and Engineering: C

View full text Add to dashboard Cite

Human amniotic membrane (HAM) was a biocompatible scaffold with advantages of anti-inflammatory, low antigen, feasibility, tolerance and low cost. In our previous work, HAM was treated to be decellularized using surfactant, lipase and DNAase methods and the efficacy as an implantable biological mesh was verified after decellularization treatment. In this work, we used the previous protocol to decellularize the fresh HAM, and applied it to repair full-thickness skin defects with Sprague-Dawley rats as the test animals. The wound healing progress was followed in the duration of 8months, and the biological repairing mechanism was explored. From the wound area alteration, white blood cell (WBC) measurements and H&E staining, dHAM was detected to promote the wound healing, comparing with the traditional clinic treatment. Immunohistochemical analyses of the bio-factors involved in the wound healing, vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1 (TGF-β1), exhibited that dHAM enhanced VEGF and α-SMA secretion but reduced TGF-β1 expression at early stage, which alleviated the wound inflammation, promoted the tissue regeneration and relieved the scar formation.

show abstract

“…Increased utilization of validated pain and scar scales are seen. 19 , 22 , 23 , 36 – 53 Vast differences among the included dressings weaken this review. Well-established dressings are being compared with novel dressings being studied for the first time without standardized means for comparison.…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis and Systematic Review of Skin Graft Donor-site Dressings with Future Guidelines

Serebrakian

Pickrell

Varon

et al. 2018

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

Background:Many types of split-thickness skin graft (STSG) donor-site dressings are available with little consensus from the literature on the optimal dressing type. The purpose of this systematic review was to analyze the most recent outcomes regarding moist and nonmoist dressings for STSG donor sites.Methods:A comprehensive systematic review was conducted across PubMed/MEDLINE, EMBASE, and Cochrane Library databases to search for comparative studies evaluating different STSG donor-site dressings in adult subjects published between 2008 and 2017. The quality of randomized controlled trials was assessed using the Jadad scale. Data were collected on donor-site pain, rate of epithelialization, infection rate, cosmetic appearance, and cost. Meta-analysis was performed for reported pain scores.Results:A total of 41 articles were included comparing 44 dressings. Selected studies included analysis of donor-site pain (36 of 41 articles), rate of epithelialization (38 of 41), infection rate (25 of 41), cosmetic appearance (20 of 41), and cost (10 of 41). Meta-analysis revealed moist dressings result in lower pain (pooled effect size = 1.44). A majority of articles (73%) reported better reepithelialization rates with moist dressings.Conclusion:The literature on STSG donor-site dressings has not yet identified an ideal dressing. Although moist dressings provide superior outcomes with regard to pain control and wound healing, there continues to be a lack of standardization. The increasing commercial availability and marketing of novel dressings necessitates the development of standardized research protocols to design better comparison studies and assess true efficacy.

show abstract

Maximizing the safety of glycerol preserved human amniotic membrane as a biological dressing

Cited by 25 publications

References 31 publications

Human Amniotic Membrane: A New Option for Graft Donor Sites – Systematic Review and Meta‐analysis

Human Amniotic Membrane: A New Option for Graft Donor Sites – Systematic Review and Meta‐analysis

The repairing of full-thickness skin deficiency and its biological mechanism using decellularized human amniotic membrane as the wound dressing

Meta-analysis and Systematic Review of Skin Graft Donor-site Dressings with Future Guidelines

Contact Info

Product

Resources

About