Maximizing the yield of lymph node cytology

Santos, Gilda da Cunha; Boerner, Scott; Geddie, William R.

doi:10.1002/cncy.20166

Cited by 25 publications

(4 citation statements)

References 17 publications

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“…Many different BBCM (PBS, saline, RPMI 1640, Hank's saline) are described in the literature for post‐collection processing of cytology samples, 1,2,5,13 while there are almost no data about the time‐ and temperature‐related influence of these media on cell morphology and biomarker immunoreactivity 2 …”

Section: Discussionmentioning

confidence: 99%

“…Cytopathologists are today more than ever before challenged with a demand to ensure sufficient and well‐preserved cellular material for multiple diagnostic, prognostic, and predictive tests using various platforms (immunocytochemistry [ICC], flow cytometry, molecular tests), 1‐3 which is leading to the introduction of new approaches in post‐collection processing of limited cytology samples. Contemporary management of cytology samples requires rapid‐on‐site evaluation (ROSE) of sample adequacy, as well as efficient triage and preparation of samples for different ancillary diagnostic methods 1,3‐5 . Rinsing the needle is another important part of post‐collection sample processing to ensure the collection of all cells obtained by the procedure 6 .…”

Section: Introductionmentioning

confidence: 99%

“…Media containing fixatives (eg, 10% neutral buffered formalin, CytoLyt™) that preserve cells and facilitate postponed preparation of cell blocks and liquid‐based‐cytology slides, and non‐fixative media, which keep cells viable only for a short period of time (a few to 72 hours) but still allow for subsequent decision, and for aliquoting and preparing samples for various ancillary methods 5,6 . Sterile normal saline, phosphate‐buffered saline (PBS), and RPMI (Roswell Park Memorial Institute) media are usually used as non‐fixative media for the collection and short‐term storage of cytology samples 1,2,4‐6 …”

Section: Introductionmentioning

confidence: 99%

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Time‐related changes in cell morphology and biomarker immunoreactivity for cells stored in a buffer‐based cell medium

Kirbiš

Prosen²,

Fležar

2021

Cytopathology

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Objective Buffer‐based cell media (BBCM) are a valuable tool in the post‐collection processing of cytology samples, though with poorly defined effects on cell properties. In this study, time‐related changes in cell morphology and biomarker immunoreactivity were evaluated for cells stored at room temperature in a BBCM prepared with bovine serum albumin (BSA) and ethylene diamine tetraacetic acid (EDTA). Methods Cytospins were prepared at five consecutive 24‐hour intervals (0, 24, 48, 72, 96) from three human cell lines (MCF7, SK‐MEL‐28, FaDu) suspended and stored in BBCM. Preservation of cell morphology was evaluated on Papanicolaou‐stained cytospins from the percentages of apoptotic cells. Preservation of immunoreactivity was evaluated for cytokeratins, oestrogen receptors, Ki67, and melanoma markers from the percentages of cells positive for the corresponding immunocytochemical reactions. Results Cell morphology was well preserved for the majority of cells of the three lines stored for 24 and 48 hours (93%, 97%, 98% and 62%, 81%, 88%, respectively), while the majority of cells were apoptotic after 72 and 96 hours (70%, 47%, 39% and 77%, 70%, 59%, respectively). The immunoreactivity of cytokeratins remained unchanged during the entire 96 hours, while that of melanoma markers (S100, HMB45, Melan‐A) decreased by 27%, 2%, and 3%, respectively. The immunoreactivity of oestrogen receptors and Ki67 decreased by 29% and 17% after the first 24 hours, and was completely lost after 96 hours. Conclusions A BBCM with the addition of BSA and EDTA facilitates good preservation of cell morphology and immunoreactivity of biomarkers for up to 48 hours at room temperature.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time‐related changes in cell morphology and biomarker immunoreactivity for cells stored in a buffer‐based cell medium

Kirbiš

Prosen²,

Fležar

2021

Cytopathology

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“…Clinicians and pathologists, as members of an oncology care team may implement tissue-sparing diagnostic algorithms based on consensus decisions custom-tailored to their patient population. For example, rapid interpretation of FNA slides is a means to ensure adequate tissue sampling while the diagnostic procedure is in progress; this requires trained personnel and can be done on-site in radiology or endoscopy suites or remotely with the help of digital imaging technology [ 26 , 27 ]. Quality control mechanisms and appropriate handling are needed to ensure tissue sample preservation and avoid exhaustion of blocks [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular classification of cancer with the 92-gene assay in cytology and limited tissue samples

et al. 2016

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BackgroundDetailed molecular evaluation of cytology and limited tissue samples is increasingly becoming the standard for cancer care. Reproducible and accurate diagnostic approaches with reduced demands on cellularity are an ongoing unmet need. This study evaluated the performance of a 92-gene assay for molecular diagnosis of tumor type/subtype in cytology and limited tissue samples.MethodsClinical validation of accuracy for the 92-gene assay in limited tissue samples such as cytology cell blocks, core biopsies and small excisions was conducted in a blinded multi-institutional study (N = 109, 48% metastatic, 53% grade II and III). Analytical success rate and diagnostic utility were evaluated in a consecutive series of 644 cytology cases submitted for clinical testing.ResultsThe 92-gene assay demonstrated 91% sensitivity (95% CI [0.84, 0.95]) for tumor classification, with high accuracy maintained irrespective of specimen type (100%, 92%, and 86% in FNA/cytology cell blocks, core biopsies, and small excisions, respectively; p = 0.26). The assay performed equally well for metastatic versus primary tumors (90% vs 93%, p = 0.73), and across histologic grades (100%, 90%, 89%, in grades I, II, and III, respectively; p = 0.75). In the clinical case series, a molecular diagnosis was reported in 87% of the 644 samples, identifying 23 different tumor types and allowing for additional mutational analysis in selected cases.ConclusionsThese findings demonstrate high accuracy and analytical success rate of the 92-gene assay, supporting its utility in the molecular diagnosis of cancer for specimens with limited tissue.

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