1981
DOI: 10.1042/bj1980621
|View full text |Cite
|
Sign up to set email alerts
|

Maximum activities and properties of glucose 6-phosphatase in muscles from vertebrates and invertebrates

Abstract: 1. The maximum catalytic activities of glucose 6-phosphatase were measured in a large number of muscles from vertebrates and invertebrates. The activities range from less than 0.1 to 8.0 mumol/min per g fresh wt. at 30 degrees C: the highest activity, observed in the flight muscle of the wasp (Vespa vulgaris), is similar to that in rat liver. The hydrolytic activity was shown to be specific towards glucose 6-phosphate. 2. The pH optimum was 6.8 and the Km was approx. 0.6 mM (flight muscle of a moth). 3. Almost… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
33
0

Year Published

1982
1982
2007
2007

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 46 publications
(34 citation statements)
references
References 14 publications
1
33
0
Order By: Relevance
“…The involvement of glucose-6-phosphatase in the regulation of glucose metabolism in muscle has been proposed by Surholt and Newsholme (1981). The strong positive correlation between the activities of hexokinase and glucose-6-phosphatase in various muscles from different animals suggests that the role of the phosphatase is to produce a substrate cycle between glucose and glucose-6-P (Fig.…”
Section: Utilization Of Carbohydrate In Flight Musclesmentioning
confidence: 91%
“…The involvement of glucose-6-phosphatase in the regulation of glucose metabolism in muscle has been proposed by Surholt and Newsholme (1981). The strong positive correlation between the activities of hexokinase and glucose-6-phosphatase in various muscles from different animals suggests that the role of the phosphatase is to produce a substrate cycle between glucose and glucose-6-P (Fig.…”
Section: Utilization Of Carbohydrate In Flight Musclesmentioning
confidence: 91%
“…That such cycling rates would be large enough in the muscles included in our study to cause significant errors in our estimates of v, and therefore v͞V max , is unlikely. With very few known exceptions (and these are not included in our sample set), glucose 6-phosphatase and fructose 1,6-bisphosphatase activities are much lower than hexokinase and phosphofructokinase activities in muscles (59). Apart from such exceptions [e.g., hawk moth (60)], mechanisms of reciprocal regulation ensure that substrate cycling rates are minimal in vivo, particularly during exercise when glycolytic flux rates are high (61,62).…”
Section: Flux Rates and Flux Capacitiesmentioning
confidence: 99%
“…In addition, we show for the first time, that long-term ex- [18]. Glucose-6-phosphatase (G6Pase) activity was assayed in HIT-T15 cell homogenates (-2.5 x lo6 cells/assay) as described in [19], except that glucose-6-phosphate was measured radiochemically by the rate of D-[1-%]glucose formation from 5 mM D-[I-"klglucose-6-phosphate (439-470 dpm/nmol) [20].…”
Section: Ceil Culture and Insulin Secretionmentioning
confidence: 99%