Maximum-likelihood estimation of sensitivity and specificity of ELISAs and faecal culture for diagnosis of paratuberculosis

Nielsen, Søren Saxmose; Grønbæk, Carsten; Agger, Jens Frederik; Houe, Hans

doi:10.1016/s0167-5877(01)00280-x

Cited by 90 publications

(63 citation statements)

References 19 publications

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“…3) [6,18]. Though this type of analysis was originally developed as a frequentist technique [11], it extends nicely to the Bayesian framework [2,21], and has been used previously in Bayesian analysis of ELISA tests [13,17]. Our method clearly outperforms the 3 SD rule, as the blue X (denoting the average ROC values of the 3 SD rule across 1,000 datasets) and the majority of the red circles (corresponding to individual simulations) lie to the lower right of the black line (our procedure).…”

Section: Simulation Studymentioning

confidence: 99%

Disease Diagnosis from Immunoassays with Plate to Plate Variability: A Hierarchical Bayesian Approach

et al. 2014

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The standard methods of diagnosing disease based on antibody microtiter plates are quite crude. Few methods create a rigorous underlying model for the antibody levels of populations consisting of a mixture of positive and negative subjects, and fewer make full use of the entirety of the available data for diagnoses. In this paper, we propose a Bayesian hierarchical model that provides a systematic way of pooling data across different plates, and accounts for the subtle sources of variations that occur in the optical densities of typical microtiter data. In addition to our Bayesian method having good frequentist properties, we find that our method outperforms one of the standard crude approaches (the "3 SD Rule") under reasonable assumptions, and provides more accurate disease diagnoses in terms of both sensitivity and specificity.

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Section: Simulation Studymentioning

confidence: 99%

Disease Diagnosis from Immunoassays with Plate to Plate Variability: A Hierarchical Bayesian Approach

et al. 2014

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“…Similar prevalence rates have been found in England and other European countries. Detection of the pathogen by isolating the organism by fecal culture is the gold standard for the diagnosis of JD [3]. However, slow growth and fastidious nature of the organism are of great impediments for regular culture test; however, discovery of M. avium complex insertion sequences, present in multiple copies provided a simple approach for characterizing the isolates of M. avium complex.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Mycobacterium avium subsp. paratuberculosis (MAP) isolates

Arjunan¹

2017

Asian J Pharm Clin Res

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Objective: Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is highly prevalent in domestic ruminants. In India, the exact prevalence of MAP genotypes still remains unknown limited, and the systematic disease control programs are also limited. This study was undertaken to study the molecular characterization of MAP isolates.Methods: About 22 MAP isolates were from cattle, sheep, and goat under gone the molecular characterization by three different methods (1) IS1311 polymerase chain reaction (PCR) with restriction enzyme analysis (REA), (2) GyrA and GyrB PCR with sequencing, and (3) digital microfluidic chip (DMC)-PCR. The study demonstrated that a) IS1311 PCR with REA (based on point mutations) identified all 22 MAP isolates as "intermediate type" irrespective of a host of origin and also belong to Indian Bison type. Molecular typing based on the gyrA and gyrB genes partial amplification and sequencing revealed that the MAP isolates exhibited more lineages toward the reference Type III, Intermediate strain. Results:The MAP isolate of sheep origin showed more lineages toward the sheep type than the isolates of cattle and goats. This variation may be due to host-pathogen interactions and adaptation to different hosts and environmental conditions in the nature. Conclusions:The DMC-PCR, which is based on sequence difference at 5` end of IS900 of MAP, differentiated rapidly all the isolates as sheep type. The application of DMC-PCR to differentiate sheep and Intermediate types is limited as the Intermediate type (Type III) and sheep type (Type I) are very closely related to each other and all the MAP isolates were confirmed as Intermediate or Type III by three different methods which are commonly present in India, Spain, and Iceland.

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“…In cattle, the sensitivity ranges from 20-73% while in sheep and goats it is much lower at 6-7%. [17][18][19] The culture technique is also hindered by the variability of bacterial growth requirements. Although it is widely known that MAP requires the use of mycobactin J for in vitro growth, several MAP field isolates have been found to be mycobactin J independent yielding a false-negative result.…”

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confidence: 99%

“…22 Specificity for the MAP ELISA ranges between 93-98% for all tested species. 17,18,23 Reports of variability in the sensitivity of the ELISA for the diagnosis of MAP-positive sera for isolates derived from different host species is not surprising since antigen preparation is not standardized. [24][25][26] Although the current challenges discussed here have been plaguing Johne disease researchers for some time, recent events in research conducted by Click et al [26][27][28][29] shed a sense of optimism on a somewhat overwhelming subject.…”

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confidence: 99%

The Johne paradigm: From detection to management to treatment

O'Shea

2011

Virulence

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Maximum-likelihood estimation of sensitivity and specificity of ELISAs and faecal culture for diagnosis of paratuberculosis

Cited by 90 publications

References 19 publications

Disease Diagnosis from Immunoassays with Plate to Plate Variability: A Hierarchical Bayesian Approach

Disease Diagnosis from Immunoassays with Plate to Plate Variability: A Hierarchical Bayesian Approach

Molecular characterization of Mycobacterium avium subsp. paratuberculosis (MAP) isolates

The Johne paradigm: From detection to management to treatment

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