MbMYBC1, a M. baccata MYB transcription factor, contribute to cold and drought stress tolerance in transgenic Arabidopsis

Abstract

Cold and drought stress considerably suppress the development of plants. In this study, a new MYB (v-myb avian myeloblastosis viral)TF gene, MbMYBC1, was isolated from the M. baccata and located in nucleus. MbMYBC1 has a positive response to low temperature and drought stress. After being introduced into Arabidopsis thaliana, the physiological indicators of transgenic Arabidopsis had corresponding changes under these two stresses, the activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) increased, electrolyte leakage rate (EL) and the content of proline increased, but the content of chlorophyll decreased. In addition, its overexpression can also activate the downstream expression of AtDREB1A, AtCOR15a, AtERD10B and AtCOR47 related to cold stress and AtSnRK2.4, AtRD29A, AtSOD1and AtP5CS1 related to drought stress. Based on these results, we speculate that MbMYBC1 can respond to cold and hydropenia signals, and can be used in transgenic technology to improve plant tolerance to low temperature and drought stress.

Overexpression of a Fragaria vesca 1R-MYB Transcription Factor Gene (FvMYB114) Increases Salt and Cold Tolerance in Arabidopsis thaliana

“…In this study, we analyzed the expression changes of four genes of the CBF transduction pathway in Arabidopsis after low-temperature treatment ( Figure 10 ). It was found that the expression of these four genes was high in transgenic lines, which was consistent with the previous research results [ 17 , 25 , 26 ]. Therefore, it is speculated that FvMYB114 can regulate the expression of cold response genes through the CBF pathway, to improve plant cold tolerance.…”

“…Jung et al found that under drought and high salt conditions, the overexpression of AtMYB44 in Arabidopsis could inhibit the expression of negative regulators ( HAB1/2 , AtPP2CA , ABI1 ) in the ABA signal pathway, thus enhancing the tolerance of A. thaliana to drought and salt, so it could grow normally in this vicious environment [ 25 ]. Moreover, MbMYBC1 can promote the expression of cold response genes such as DREB1A , COR15a , ERD10B and COR47 under cold stress [ 26 ].…”

Harnessing Phytohormones: Advancing Plant Growth and Defence Strategies for Sustainable Agriculture

“…In such investigations, beyond merely conducting enzymatic reactions or volatile organic compound (VOC) profiling, employing transcriptomics and proteomics techniques allows for the analysis of alterations in gene expression or protein abundance resulting from phytohormone treatments or genetic modifications (Zhu et al, 2019;Yang et al, 2023;Gupta et al, 2024;Hu et al, 2024;Kumari et al, 2024;Xiao et al, 2024). Through this comprehensive approach, researchers can attain a deeper understanding of the molecular mechanisms governing hormone regulation (Liu et al, 2023). This knowledge can then be leveraged through techniques such as RNA interference (RNAi), CRISPR/Cas, overexpressions and/or their combination to engineer plants by altering the expression of enzymes or regulatory genes involved in phytohormone metabolism (Yang et al, 2008;Abe and Ichikawa, 2016;Liu et al, 2023).…”

Deciphering the roles of unknown/uncharacterized genes in plant development and stress responses

“…The presence of conserved domains helps researchers predict the roles of U/U genes and provides direction for the functional research of unknown genes. Exogenously overexpressing MbMYBC1 and MbMYB108 from Malus baccata enhanced the cold and drought resistance of transgenic Arabidopsis (Arabidopsis thaliana) (Yao et al, 2022;Liu et al, 2023). Exogenously overexpressing FvMYB82 from strawberry (Fragaria vesca) and the R1-MYB transcription factor gene LcMYB1 from sheepgrass (Leymus chinensis (Trin.)…”