MCF-7 ve PC-3 Hücre Hatlarında, Hücre Canlılığı ve DNA Hasarı Üzerine TRPV4 Antagonisti RN 1734'ün Etkileri

Çakır, Murat; Erden, Yavuz

doi:10.16919/bozoktip.588740

Cited by 2 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cakır and Erden conducted a study in which RN-1734 at concentrations of 50 and 100 μM in human prostate (PC-3) and human breast (MCF-7) cancer cell lines caused DNA damage in the cells and significantly reduced cell viability (%) 14 . Bahari et al also found that the administration RN-1734 at concentrations of 1, 10, and 100 μM in the colorectal cancer cell line statistically decreased cell viability (%) 31 .…”

Section: Discussionmentioning

confidence: 99%

“…Fang et al found that inhibition of TRPV4 channels in human hepatocellular carcinoma cells suppresses cell proliferation, induces apoptosis, and reduces migration ability 13 . In addition, Çakır and Erden found that the administration of RN-1734, a TRPV4 antagonist, in human prostate (PC-3) and human breast (MCF-7) cancer cell lines caused DNA damage in cells and reduced cell viability 14 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytotoxic activity of TRPV4 antagonist RN-1734 in G-361 human melanoma cancer cell line

GÜLEŞ,

BİLİCİ,

KURBASEVIC

et al. 2023

Cukurova Medical Journal

View full text Add to dashboard Cite

Purpose: Intracellular calcium (Ca2+) signaling plays a role in many cellular events, such as cell proliferation and differentiation, gene transcription, oxidative stress, the antioxidant system, and apoptosis. Transient receptor potential vanilloid 4 (TRPV4) channels are non-selective cation (Ca2+) channels. The present study aims to investigate the cytotoxic activity of RN-1734, a transient receptor potential vanilloid 4 (TRPV4) antagonist, in the G361 human melanoma cancer cell line. Materials and Methods: The effects of RN-1734 on G361 cell viability at concentrations of 1, 5, 25, 50, and 100 μM were measured using the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) method. Total antioxidant status (TAS) and total oxidant status (TOS) levels were determined using a ready-made commercial kit, after which oxidative stress index (OSI) values were calculated. To determine the apoptotic effects of RN-1734, Bcl-2, Bax, and p53 expression levels, caspase-3 and -8 activities were examined via quantitative real-time PCR analysis. Results: G361 cell viability significantly decreased to 82.72, 72.81, 56.36, 39.16 and 18.96% in RN-1734 groups (1, 5, 25, 50 and 100 μM) compared to the control group (100.00%). At IC50 concentration (39.48 μM), RN-1734 application (3.35 mmol/g prot.-TAS, 45.87 μmol/g prot.-TOS, and 1501.97 AU-OSI) increased the TAS level (2.17 mmol/g prot.) and decreased the TOS level (55.41 μmol/g prot.) and OSI value (3142.76 AU) compared to the control group. Conclusion: Our findings show that RN-1734 may be a novel therapeutic approach to treating melanoma by decreasing the cell viability of G361 human melanoma cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic activity of TRPV4 antagonist RN-1734 in G-361 human melanoma cancer cell line

GÜLEŞ,

BİLİCİ,

KURBASEVIC

et al. 2023

Cukurova Medical Journal

View full text Add to dashboard Cite

show abstract

“…Pollutants, including pesticides, are known to spread quickly in the environment and human organs, leading to long-term health issues shortly after their introduction (Gavrilescu et al, 2015). Because of their significant metabolic activities, the liver is the main target organ for pesticide damage (Çakır and Erden, 2019). Hence, it is a global priority to protect human health from pesticides, pesticide-contaminated drinking water, and harmful air pollutants (Damalas and Koutroubas, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of Clethodim Exposure on Liver Cells&

Ergenekon,

Erman,

Şimşek Özek

2024

Türk Tarım Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

Clethodim, a herbicide group pesticide, is classified as a toxicity class II agent. The development toxicity, immunotoxicity, neurotoxicity and reproductive toxicity of this herbicide have been demonstrated in zebrafish and rat. There are no in vitro studies on the hepatoxic effects of clethodim. In this study, for the first time, clethodim was tested with liver cell line models. THLE-2 and B129 cells were treated with a wide range of clethodim for 96 hours. After that, its hepatoxic effects were evaluated by MTT and SRB analyses. Both assays revealed that the cellular proliferation was strikingly decreased with the dose dependent manner. Especially, 1000 µg/ml dose of the clethodim exposure supressed the cellular proliferation at a %90 and %75 for B129 and THLE-2 cells. Moreover, a significant reduction was acquired in both cells number and volume with the high doses of this cyclohexanedione class herbicide. These cytotoxic impacts can be strongly related to the herbicide elaved oxidative stress. In the light of our results, the long and high doses of clethodim exposure has a hepatoxic effect and its toxic target organ is be liver. Hence, further studies on the use of clethodim-based herbicides are urgently needed.

show abstract

MCF-7 ve PC-3 Hücre Hatlarında, Hücre Canlılığı ve DNA Hasarı Üzerine TRPV4 Antagonisti RN 1734'ün Etkileri

Cited by 2 publications

References 22 publications

Cytotoxic activity of TRPV4 antagonist RN-1734 in G-361 human melanoma cancer cell line

Cytotoxic activity of TRPV4 antagonist RN-1734 in G-361 human melanoma cancer cell line

Cytotoxic Effect of Clethodim Exposure on Liver Cells&

Contact Info

Product

Resources

About