mcr-1 is borne by highly diverse Escherichia coli isolates since 2004 in food-producing animals in Europe

Garch, Farid El; Sauget, Marlène; Hocquet, Didier; LeChaudee, D.; Woehrle, Frédérique; Bertrand, Xavier

doi:10.1016/j.cmi.2016.08.033

Cited by 68 publications

(43 citation statements)

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“…ExPEC strains in these clonal groups may be derived from DEC strains and may have evolved into ExPEC through the accumulation of virulence factors (Brzuszkiewicz et al, 2006). Some regional monitoring investigations showed that CC10 strains from clinical patients, birds, and swine were associated with ciprofloxacin resistance and carried various CTX-M-type genes and mcr-1 (mediated colistin resistance) (Oteo et al, 2009;Giufre et al, 2012;Ramos et al, 2013;EI et al, 2017). The widespread dissemination of CC10 E. coli across wide areas, plus multidrug resistance, may lead to a public emergence similar to the ST131 lineage infections.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and genetic diversity of human diarrheagenic Escherichia coli isolates by multilocus sequence typing

Chen

Zheng

et al. 2018

International Journal of Infectious Diseases

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Section: Discussionmentioning

confidence: 99%

Prevalence and genetic diversity of human diarrheagenic Escherichia coli isolates by multilocus sequence typing

Chen

Zheng

et al. 2018

International Journal of Infectious Diseases

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“…The mcr-1 -positive gut bacteria included Escherichia coli ( E. coli ) [21, 22], S. enterica [23, 24], K. pneumoniae [6, 25, 26], Enterobacter aerogenes ( E. aerogenes ) [27], Kluyvera ascorbata [28], Citrobacter freundii [29], and Citrobacter braakii [30]. Not only does the mcr-1 gene appear in diversified E. coli isolates with different sequence types [20, 31], but also it coexists with the NDM-1 [32, 33] and its variants, like NDM-5 [34, 35] and NDM-9 [36]. Additionally, ESBL can be coproduced with MCR-1 from a single plasmid or a same bacterial isolate [37–39].…”

Section: Introductionmentioning

confidence: 99%

Expanding landscapes of the diversified mcr-1-bearing plasmid reservoirs

Wang

Sun

et al. 2017

Microbiome

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BackgroundPolymyxin is a cationic polypeptide antibiotic that can disrupt bacterial cell membrane by interacting with its lipopolysaccharide molecules and is used as a last resort drug against lethal infections by the carbapenem-resistant superbugs (like NDM-1). However, global discovery of the MCR-1 colistin resistance dramatically challenges the newly renewed interest in colistin for clinical use.MethodsThe mcr-1-harboring plasmids were acquired from swine and human Escherichia coli isolated in China, from 2015 to 2016, and subjected to Illumina PacBio RSII and Hi-Seq2000 for full genome sequencing. PCR was applied to close the gap of the assembled contigs. Ori-Finder was employed to predict the replication origin (oriC) in plasmids. The phenotype of MCR-1-producing isolates was evaluated on the LBA plates with various level of colistin. Genetic deletion was used to test the requirement of the initial “ATG” codon for the MCR-1 function.ResultsHere, we report full genomes of over 10 mcr-1-harboring plasmids with diversified replication incompatibilities. A novel hybrid IncI2/IncFIB plasmid pGD17-2 was discovered and characterized from a swine isolate with colistin resistance. Intriguingly, co-occurrence of two unique mcr-1-bearing plasmids (pGD65-3, IncI2, and pGD65-5, IncX4) was detected in a single isolate GD65, which might accelerate dissemination of the mcr-1 under environmental selection pressure. Genetic analyses of these plasmids mapped mobile elements in the context of antibiotic resistance and determined two insertion sequences (ISEcp1 and ISApl1) that are responsible for the mobilization of mcr-1. Gene deletion also proved that the first ATG codon is redundant in the mcr-1 gene.ConclusionsCollectively, our results extend landscapes of the diversified mcr-1-bearing plasmid reservoirs.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-017-0288-0) contains supplementary material, which is available to authorized users.

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“…On the other hand, MLST analysis from sequence reads identified the sequence types ST132, ST48, ST4419, ST522, and ST10 (Table 1). In particular, the ST10 has been widely identified in animal, food, human, and environmental samples and associated with the production of CTX-M-type ESBLs and, more recently, the MCR-1 enzyme, denoting great versatility of this lineage for adaptation to different hosts (3,(19)(20)(21)(22)(23). Furthermore, the presence of IncX4 plasmids carrying the mcr-1 gene in E. coli isolates CF1.2, CF131, and CF132 was confirmed, as previously reported in human and animal clinical samples collected in this region (3,19).…”

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confidence: 99%

Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America

Monte

Mem

Fernandes

et al. 2017

Antimicrob Agents Chemother

127

111

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The detection and rapid spread of colistin-resistant Enterobacteriaceae carrying the mcr-1 gene has created an urgent need to strengthen surveillance. In this study, eight clonally unrelated colistin-resistant Escherichia coli isolates carrying mcr-1 and bla CTX-M or bla CMY-2 genes were isolated from commercial chicken meat in Brazil. Most E. coli strains carried IncX4 plasmids, previously identified in human and animal isolates. These results highlight a new reservoir of mcr-1-harboring E. coli strains in South America.

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mcr-1 is borne by highly diverse Escherichia coli isolates since 2004 in food-producing animals in Europe

Abstract: MCR-1 was detected yearly in European food-producing animal since 2004 with a high diversity of pulsotypes supporting the dissemination of mcr-1 via plasmids.

Cited by 68 publications

References 11 publications

Prevalence and genetic diversity of human diarrheagenic Escherichia coli isolates by multilocus sequence typing

Prevalence and genetic diversity of human diarrheagenic Escherichia coli isolates by multilocus sequence typing

Expanding landscapes of the diversified mcr-1-bearing plasmid reservoirs

Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America

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