MCR-5-Producing Colistin-Resistant Cupriavidus gilardii Strain from Well Water in Batna, Algeria

Cherak, Zineb; Loucif, Lotfi; Khedher, Mariem Ben; Moussi, A.; Benbouza, Amel; Baron, Sophie Alexandra; Rolain, Jean‐Marc

doi:10.1128/msphere.00575-21

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…followed by unclassified members of Xanthomonadales, Dokdonella, Pseudomonas, and Hydrogenophilales. Although many of the potential mcr- 5 host identified in this study have not been associated with this variant, it has been observed in Stenotrophomonas isolated from poultry farm waste and Cupriavidus isolated from a clinical case and well water. , Both Stenotrophomonas and Cupriavidus genera contain species that are considered emerging global pathogens while others, such as species within the Comamonas and Morganella genera, have been shown to occasionally cause infection in immunocompromised individuals. , …”

Section: Resultsmentioning

confidence: 61%

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Isanovic,

Correa Velez,

Viner

et al. 2024

ACS EST Water

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To address the growing threat of global antimicrobial resistance, a one-health approach is needed to understand the complex socioecological cycling of antibiotic resistance genes. In this study, a metagenomics approach using DNA shotgun sequencing, metagenome assembly, and antibiotic resistance gene (ARG) annotation was used to examine seasonal patterns in the abundance of mobile colistin resistance (mcr) gene variants in bioaerosols and liquid sludge in three wastewater treatment plants (WWTPs). ARGs represented 0.2−0.8 and 0.1−0.2% of the bioaerosol and liquid sludge metagenomes, respectively, while mcr genes represented 0−0.3 and 0−0.5% of the identified ARGs in bioaerosol and liquid sludge metagenomes. Seven of the ten known mcr variants were detected in wastewater bioaerosol and liquid samples, with mcr-5 and mcr-8 being the most prevalent across all seasons and sites. Additional functional and taxonomic annotation of mcr-containing metagenomic contigs showed that mcr genes were often located on contigs with other co-occurring ARGs and mobile genetic elements and may be harbored by opportunistic human pathogens and other bacterial taxa not previously associated with mcr genes. Atmospheric dispersion modeling showed that mcr-containing bioaerosols can be transported kilometers away from the WWTPs, resulting in the possible dissemination of these ARGs into surrounding environments and communities.

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Section: Resultsmentioning

confidence: 61%

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Isanovic,

Correa Velez,

Viner

et al. 2024

ACS EST Water

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“…However, the mobile colistin resistance mechanism was also detected via the isolation of one mcr-5 -producing Cupriavidus gilardii strain from the well water of the maternity hospital. The whole genome of this strain was sequenced and has already been published [ 12 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, colistin-resistant enterobacteria isolated from tap water was potentially related to mutational mechanisms. In addition, we reported the first mcr-5 positive Cupriavidus gilardii through its isolation from well water [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Dissemination of Carbapenemases and MCR-1 Producing Gram-Negative Bacteria in Aquatic Environments in Batna, Algeria

et al. 2022

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Antibiotic-resistant-bacteria are being considered as emerging environmental contaminants where the importance of the surrounding environment in their emergence and dissemination has been emphasized. The aim of this study was to screen for the presence and diversity of carbapenem- and colistin-resistant Gram-negative bacteria (GNBs) in different aquatic environments. Water samples were collected in Batna, Algeria. Carbapenem- and colistin-resistant GNBs were selectively isolated and then identified using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. After phenotypic antibiotic susceptibility testing, the molecular mechanisms of β-lactams and colistin-resistance were investigated by PCR and sequencing. The clonality of mcr-1 positive Escherichia coli was determined by multi-locus sequence typing. We noticed a high level of resistance in both tap water and wastewater. The most commonly found carbapenem-resistance mechanism was the OXA-48 enzyme, but other carbapenemases were also detected. In addition, the mcr-1 gene was detected in 18 E. coli of different sequence types. Our findings highlight the role of aquatic environments in the dissemination of resistant-bacteria, especially considering that water is a connecting medium between different ecological systems and can easily transmit resistant-bacteria and promote horizontal gene transfer. Thus, the development of effective treatment strategies for eliminating antibiotic-resistance is seriously needed.

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“… 9 There are currently no recommendations for antimicrobial use to treat C. gilardii infections. 8 Moreover, the high case fatality rate of C. gilardii infections may be related to the characteristics of acquired drug resistance. 8 , 10 Compared with other clinically well-known pathogens, we know very little about the genome, clinical characteristics, and therapy of C. gilardii .…”

Section: Introductionmentioning

confidence: 99%

Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms

Zhao,

Yang,

Huang

et al. 2024

IDR

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Purpose Cupriavidus gilardii is an emerging multidrug-resistant pathogen found in many environments and few clinical samples. The clinical infectiousness, pathogenicity, and resistance mechanisms of C. gilardii are still unclear due to the lack of clinical and sequencing data. We need to obtain insight into the clinical characteristics, virulence, and resistance mechanisms of C. gilardii . Patients and Methods We isolated five C. gilardii isolates from hospitalized patients and carried out assay, culture and genome sequencing. We analyzed the genomic features of clinical C. gilardii isolates and took insight into their clinical characteristics, virulence, and resistance mechanisms. Results These isolates were resistant to meropenem, gentamicin, and other antimicrobials due to intrinsic resistance genes. Furthermore, the sequencing results revealed the widespread presence of the MCR-5.1 gene in C. gilardii . The virulence magnitude of C. gilardii is closely correlated with the number of virulence factors they carry. Some C. gilardii strains can acquire resistance to levofloxacin through gyrA gene mutation during treatment. The diverse antimicrobial resistance mechanisms challenge the treatment of C. gilardii infections. Conclusion We present the genomic characteristics of clinically isolated C. gilardii to improve (i) our understanding of this pathogen and (ii) treatment options.

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MCR-5-Producing Colistin-Resistant Cupriavidus gilardii Strain from Well Water in Batna, Algeria

Abstract: Colistin resistance mediated by mcr genes in Gram-negative bacteria has gained significant attention worldwide. This is due to the ability of these genes to be horizontally transferred between different bacterial genera and species.

Cited by 7 publications

References 16 publications

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Dissemination of Carbapenemases and MCR-1 Producing Gram-Negative Bacteria in Aquatic Environments in Batna, Algeria

Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms

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