Md<scp>MYB</scp>46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

Chen, Keqin; Song, Mengru; Guo, Yunna; Liu, Lifu; Hou, Xue; Hirata, Dai; Zhang, Zhihong

doi:10.1111/pbi.13151

Cited by 174 publications

(99 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MdSND1 was able to activate the transcription of MdMYB46/83 and indirectly regulate the accumulation of lignin in apple. However, whether SND1 can function like MdMYB46 and directly act on the promoter of lignin biosynthesis genes remains unknown 36 . Both drought and salt stress can lead to changes in osmotic pressure in cells, while salt also has additional ionic or iontoxicity effects 1,38 .…”

Section: Discussionmentioning

confidence: 99%

Dual role of MdSND1 in the biosynthesis of lignin and in signal transduction in response to salt and osmotic stress in apple

et al. 2020

Self Cite

View full text Add to dashboard Cite

Clarifying the stress signal transduction pathway would be helpful for understanding the abiotic stress resistance mechanism in apple (Malus × domestica Borkh.) and could assist in the development of new varieties with high stress tolerance by genetic engineering. The key NAC transcription factor SND1, which is involved in the lignin biosynthesis process in apple, was functionally analyzed. The results of the stress treatments indicated that MdSND1 could be induced by salt, mannitol and ABA. Compared with wild-type GL-3 plants, MdSND1-overexpressing apple plants with greater antioxidant capacity and lignin were more resistant to salt and simulated osmotic stress, while RNAi plants were more vulnerable. Additionally, molecular experiments confirmed that MdSND1 could regulate the biosynthesis of lignin by activating the transcription of MdMYB46/83. Moreover, genes known to be involved in the stress signal transduction pathway (MdAREB1A, MdAREB1B, MdDREB2A, MdRD29A, and MdRD22) were screened for their close correlations with the expression of MdSND1 and the response to salt and osmotic stress. Multiple verification tests further demonstrated that MdSND1 could directly bind to these gene promoters and activate their transcription. The above results revealed that MdSND1 is directly involved in the regulation of lignin biosynthesis and the signal transduction pathway involved in the response to both salt and osmotic stress in apple.

show abstract

Section: Discussionmentioning

confidence: 99%

Dual role of MdSND1 in the biosynthesis of lignin and in signal transduction in response to salt and osmotic stress in apple

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, transcriptional upregulation of lignin biosynthesis genes such as C4H , C3H , CAD , F5H , HCT , 4CL , COMT , CCR , and CCoAOMT results in the deposition of lignin, thickening of the secondary cell wall, and enhanced salt and osmotic resistance in white birch ( Betula platyphylla ) and apple ( Malus domestica Borkh.) (Chen et al, 2019b; Hu et al, 2019c). Elicitation of the expression of lignin biosynthesis genes such as PAL and CAD in Miscanthus and 4CL1 in loquat fruit ( Eribotrya japonica ; Domon et al, 2013; Zhang et al, 2020c), and the consequent accumulation of lignin may help plants to acclimate to cold environments.…”

Section: Biofunctions Of Phenylpropanoid Metabolitesmentioning

confidence: 99%

“…In transgenic A. thaliana and apple ( M. domestica Borkh. ), MdMYB46 increases the deposition of lignin and secondary cell wall biosynthesis by directly binding to the promoters of lignin biosynthesis‐related genes such as CAD , COMT , and CCR (Chen et al, 2019b). The promoter of 4CL , a key lignin biosynthesis gene, is activated by MYB TFs, resulting in the induction of lignin biosynthesis.…”

Section: Regulation Of the Biosynthesis Of Phenylpropanoid Metabolitesmentioning

confidence: 99%

Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions

Dong

Lin

2021

JIPB

839

429

View full text Add to dashboard Cite

Phenylpropanoid metabolism is one of the most important metabolisms in plants, yielding more than 8,000 metabolites contributing to plant development and plant-environment interplay. Phenylpropanoid metabolism materialized during the evolution of early freshwater algae that were initiating terrestrialization and land plants have evolved multiple branches of this pathway, which give rise to metabolites including lignin, flavonoids, lignans, phenylpropanoid esters, hydroxycinnamic acid amides, and sporopollenin. Recent studies have revealed that many factors participate in the regulation of phenylpropanoid metabolism, and modulate phenylpropanoid homeostasis when plants undergo successive developmental processes and are subjected to stressful environments. In this review, we summarize recent progress on elucidating the contribution of phenylpropanoid metabolism to the coordination of plant development and plantenvironment interaction, and metabolic flux redirection among diverse metabolic routes. In addition, our review focuses on the regulation of phenylpropanoid metabolism at the transcriptional, post-transcriptional, post-translational, and epigenetic levels, and in response to phytohormones and biotic and abiotic stresses.

show abstract

“…However, the complex overlapping network of regulatory mechanisms underlying stress tolerance is poorly understood (Munns and Tester, 2008). Recently, high‐throughput sequencing and bioinformatics have disclosed transcription factors that participate in the salt stress response by regulating downstream gene expression (Chen et al ., 2019; Nakashima et al ., 2012; Wang et al ., 2019a).…”

Section: Introductionmentioning

confidence: 99%

The miR156/SPL module regulates apple salt stress tolerance by activating MdWRKY100 expression

Hou

Zhang

et al. 2020

Plant Biotechnology Journal

Self Cite

149

View full text Add to dashboard Cite

Summary Salt stress dramatically impedes plant growth and development as well as crop yield. The apple production regions are reduced every year, because of the secondary salt damage by improper fertilization and irrigation. To expand the cultivation area of apple (Malus domestica) and select salt‐resistant varieties, the mechanism of salt tolerance in apple is necessary to be clarified. The miR156/SPL regulatory module plays key roles in embryogenesis, morphogenesis, life cycle stage transformation, flower formation and other processes. However, its roles in the mechanisms of salt tolerance are unknown. In order to elucidate the mechanism of 156/SPL regulating salt stress in apple, we performed RLM‐5’ RACE and stable genetic transformation technology to verify that both mdm‐MIR156a and MdSPL13 responded to salt stress in apple and that the latter was the target of the former. MIR156a overexpression weakened salt resistance in apple whereas MdSPL13 overexpression strengthened it. A total of 6094 differentially expressed genes relative to nontransgenic apple plants were found by RNA‐Seq analysis of MdSPL13OE. Further verification indicated that MdSPL13 targeted the MdWRKY100 gene promoter. Moreover, MdWRKY100 overexpression enhanced salt tolerance in apple. Our results revealed that the miR156/SPL module regulates salt tolerance by up‐regulating MdWRKY100 in apple. This study is the first to elucidate the mechanism underlying the miRNA network response to salt stress in apple and provides theoretical and empirical bases and genetic resources for the molecular breeding of salt tolerance in apple.

show abstract

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

Cited by 174 publications

References 74 publications

Dual role of MdSND1 in the biosynthesis of lignin and in signal transduction in response to salt and osmotic stress in apple

Dual role of MdSND1 in the biosynthesis of lignin and in signal transduction in response to salt and osmotic stress in apple

Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions

The miR156/SPL module regulates apple salt stress tolerance by activating MdWRKY100 expression

Contact Info

Product

Resources

About