MDM2 mRNA Expression in the p53 Pathway May Predict the Potential of Invasion and Liver Metastasis in Colorectal Cancer

“…This polymorphism is not the only factor involved in colorectal cancer development, but it might act as an enhancer of the progress, as MDM2 has been seen to interact with many genes involved in cancer development [2,3,29,42].…”

Section: Discussionmentioning

confidence: 99%

Impact of MDM2 polymorphism

Chaar

Arfaoui

Amine

et al. 2012

European Journal of Gastroenterology & Hepatology

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“…Our study confirmed that methylation of the p14 ARF promoter might be responsible for the silencing of p14 ARF in some glioma patients, and thus it could be used as a biomarker for glioma [33]. Although the Kaplan-Meier survival curve suggested that the post-2-year survival rate is worse in patients with methylation in the p14 ARF promoter than in those without methylation, no correlation was found between methylation of the p14 ARF promoter and the grade of the disease or the prognosis.…”

Section: Discussionsupporting

confidence: 89%

p14ARF promoter region methylation as a marker for gliomas diagnosis

Qiao

Zhu

2010

Med Oncol

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Methylation in the promoter region is one of the mechanisms through which tumor suppressors are inactivated, resulting in tumorigenesis and/or tumor progression. Herein, we studied the methylation status in the promoter region of the p14ARF tumor suppressor gene in 33 brain tissues isolated from glioma patients (astrocytomas) and compared to 12 brain tissues isolated from autopsy donors using methylation-specific polymerase chain reaction (MSP). The correlation between the expression of P14 and P53 was investigated using immunohistochemistry (IHC). The average percentage of methylation in the promoter region of p14ARF gene in brain samples from glioma patients is 39.4%, while 0 from autopsy donors. No difference in the methylation level between low-grade and high-grade gliomas was detected. The methylation status has no correlation with the prognosis in glioma patients. A significant correlation between the expression of mutant form of TP53 and the grade of the glioma was established. Furthermore, there was a negative correlation between methylation of the p14ARF promoter and the expression of the mutant form of TP53. Therefore, our data suggest that methylation in the promoter region of the p14ARF gene may be used as a biomarker for the diagnosis of gliomas.

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“…Publications dealing with colon cancer have reported gene expression studies in metastatic diseases [34,35]. However, the stability of the reference gene expression in metastatic and non-metastatic primary tumours remains crucial.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Sørby

Andersen

Bukholm

et al. 2010

J Exp Clin Cancer Res

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BackgroundReal-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity. The gene expression is quantified by normalization to one or more reference genes which are presumed stably expressed throughout a given experiment. The aim of this study was to validate a standardized experimental setup to identifying reference genes for normalization of qRT-PCR in the metastatic and non-metastatic colon cancer.MethodsIn this study, expression of 16 commonly used reference genes was quantified in tumour tissue and individual-matched normal mucosa in 18 non-metastatic colon cancer patients and 20 colon cancer patients with distant metastases using TaqMan Low Density Array (TLDA). The expression stability was determined and compared by means of geNorm and NormFinder.ResultsTwo pairs of genes, HPRT1/PPIA and IPO8/PPIA, were identified to be suitable to normalize gene expression data in metastatic and non-metastatic colon cancer patients, according to geNorm and NormFinder respectively.ConclusionWe propose a standardized approach of finding the most suitable reference gene(s) in every qRT-PCR experiment using TLDA.

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MDM2 mRNA Expression in the p53 Pathway May Predict the Potential of Invasion and Liver Metastasis in Colorectal Cancer

Abstract: The results suggest that disruption of the p53/MDM2/p14ARF pathway may frequently participate in colonic carcinogenesis and that MDM2 expression status may be a factor in the prediction of potential invasion and liver metastasis of colorectal carcinomas.

Cited by 24 publications

References 33 publications

Impact of MDM2 polymorphism

Impact of MDM2 polymorphism

p14ARF promoter region methylation as a marker for gliomas diagnosis

Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

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