MdMYB52 regulates lignin biosynthesis upon the suberization process in apple

Xu, Xuan; Guerriero, Gea; Berni, Roberto; Sergeant, Kjell; Guignard, Cédric; Lenouvel, Audrey; Hausman, Jean-François; Legay, Sylvain

doi:10.3389/fpls.2022.1039014

Cited by 9 publications

(8 citation statements)

References 70 publications

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“…Supporting even a broader role for MYB68, it has been recently proposed to regulate suberin deposition in the endodermis and during apple fruit russeting (Xu et al, 2022;Xu et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…The notion that orthologs of MYB68 are expressed in cork cells of the stems of several trees and upon wounding in potato tubers goes along with the idea that there are conserved mechanisms between root, stem, and wound-induced cork differentiation. Supporting even a broader role for MYB68, it has been recently proposed to regulate suberin deposition in the endodermis and during apple fruit russeting (Xu et al ., 2022; Xu et al ., 2023). Finally, our work provides novel targets to engineer and manipulate barrier properties, such as the number of barrier cell layers and polymer deposition.…”

Section: Discussionmentioning

confidence: 99%

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MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

Molina,

Horvath,

Zhang

et al. 2024

Preprint

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Plants have developed specialized barriers to protect and isolate the inner tissues from the environment while maintaining homeostasis. Different barriers are present in various organs and at different growth stages. During secondary growth, the periderm acts as the protective tissue, covering roots, stems, and branches as they become thick. The periderm is a dynamic barrier comprising a stem cell niche known as the cork cambium, which bifacially divides to generate the phelloderm inward and the cork outward. Cork cells have a unique cell wall impregnated with suberin and lignin polymers, essential for the barrier function. Despite its importance, the differentiation process that forms new cork cells from the stem cell is largely unknown. In this work, we identify members of the MYB36-subclade transcription factors as key regulators of cork differentiation. On the one hand, this set of transcription factors promotes suberin deposition by inducing the expression of enzymes involved in all steps of suberin biosynthesis, including the recently discovered suberin-polymerizing enzymes GDS Lipases; on the other hand, it represses cork cambium proliferation. Furthermore, we demonstrated that suberin deposition in the cork is a robust process regulated by a complex network of transcription factors, including other MYB transcription factors that activate suberin deposition in the endodermis. However, only members of the MYB36 subclade can repress cell proliferation in different developmental contexts, highlighting general and specific functions for MYB transcription factors. These findings have broad applicability, as tissue-specific manipulation of MYB activity has the potential for improving traits of biotechnological interest, such as thicker periderms and more suberized cork layers, and for assessing how these traits affect plant performance in response to stresses.

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“…Supporting even a broader role for MYB68, it has been recently proposed to regulate suberin deposition in the endodermis and during apple fruit russeting (Xu et al, 2022;Xu et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

Molina,

Horvath,

Zhang

et al. 2024

Preprint

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“…In this work, we propose a dual role of MYB68 as a regulator of radial suberin patterning and cell lineage specification within the root endodermis. MYB68 was recently found to activate suberin-related promoters via an in-planta assay (Molina, et al, 2024) and enable suberization in rusetting of apples (Xu, et al, 2023). Thus, it likely plays a role in controlling expression of genes involved in production of suberin and thereby influences the suberization within the individual endodermal cells.…”

Section: Discussionmentioning

confidence: 99%

MYB68 regulates radial endodermal differentiation and suberin patterning

Kraska,

Mercadal Melia,

Nakano

et al. 2024

Preprint

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Roots are composed of concentric tissue layers that enable selective uptake of nutrients, attributed to the vascular-embracing endodermis. In mature root regions, endodermal cells coat their cell surface in a hydrophobic polymer termed suberin, which blocks solute transport across the plasma membrane. Intriguingly, specific cells adjacent to the water-conducting xylem remain unsuberized. These are termed passage cells based on the assumption that they create a passage for nutrient influx into vasculature in the otherwise sealed-off root regions. The identity and function of passage cells remain unknown, but their presence proposes the existence of individual identities within the endodermis. In this study, we probe this by performing an in-depth investigation of passage cells and endodermal suberization in the model plant Arabidopsis thaliana. Our work identifies the transcription factor MYB68 as a key regulator of suberization and endodermal lineage-specification related to passage cell formation. Through transcriptional profiling of myb68 mutants, we unravel genes with passage cell-associated expression that suggest a function in cation homeostasis. Collectively, our findings create a framework for a deeper understanding of radial cell identities within the endodermis and highlight that dynamic spatiotemporal patterning is an important physiological feature of root ground tissues.

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“…In Nicotiana benthamiana leaves, the transient expression of apple MdMYB93 triggered a massive increased expression of genes belonging to the phenylpropanoid and lipid metabolisms with a concomitant deposition of suberin in cell walls. Recently, we described MdMYB52, which is specifically expressed in russeted tissues and regulates the biosynthesis of G-units lignin associated with the aromatic domain of suberin ( Xu et al., 2022b ). Other members of the MYB family were never studied in apple fruit although several other suberin transcriptional regulators were recently discovered in A. thaliana , including MYB9, MYB36 MYB107, MYB53, MYB92 and MYB39/SUBERMAN to name a few ( Kamiya et al., 2015 ; Lashbrooke et al., 2016 ; Cohen et al., 2020 ; To et al., 2020 ; Shukla et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of MdMYB68, a suberin master regulator in russeted apples

Guerriero

Domergue

et al. 2023

Front. Plant Sci.

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IntroductionApple russeting is mainly due to the accumulation of suberin in the cell wall in response to defects and damages in the cuticle layer. Over the last decades, massive efforts have been done to better understand the complex interplay between pathways involved in the suberization process in model plants. However, the regulation mechanisms which orchestrate this complex process are still under investigation. Our previous studies highlighted a number of transcription factor candidates from the Myeloblastosis (MYB) transcription factor family which might regulate suberization in russeted or suberized apple fruit skin. Among these, we identified MdMYB68, which was co-expressed with number of well-known key suberin biosynthesis genes.MethodTo validate the MdMYB68 function, we conducted an heterologous transient expression in Nicotiana benthamiana combined with whole gene expression profiling analysis (RNA-Seq), quantification of lipids and cell wall monosaccharides, and microscopy.ResultsMdMYB68 overexpression is able to trigger the expression of the whole suberin biosynthesis pathway. The lipid content analysis confirmed that MdMYB68 regulates the deposition of suberin in cell walls. Furthermore, we also investigated the alteration of the non-lipid cell wall components and showed that MdMYB68 triggers a massive modification of hemicelluloses and pectins. These results were finally supported by the microscopy.DiscussionOnce again, we demonstrated that the heterologous transient expression in N. benthamiana coupled with RNA-seq is a powerful and efficient tool to investigate the function of suberin related transcription factors. Here, we suggest MdMYB68 as a new regulator of the aliphatic and aromatic suberin deposition in apple fruit, and further describe, for the first time, rearrangements occurring in the carbohydrate cell wall matrix, preparing this suberin deposition.

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MdMYB52 regulates lignin biosynthesis upon the suberization process in apple

Cited by 9 publications

References 70 publications

MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

MYB68 regulates radial endodermal differentiation and suberin patterning

Characterization of MdMYB68, a suberin master regulator in russeted apples

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