MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple

Wang, Qing; Liu, Heng; Zhang, Min; Liu, Shaohua; Zhang, Yuanhu

doi:10.3389/fpls.2020.512844

Cited by 17 publications

(21 citation statements)

References 50 publications

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“…In this study, 344 TFs consisting mainly of bHLH (76 genes), ERF (68 genes), MYB (52 genes), and WRKY (30 genes) were differentially expressed in response to H. armigera infestation ( Supplementary Table 4 ), which might contribute to the activation of different pathways of cotton plants to initiate sophisticated defense responses against H. armigera . TFs are involved in the biosynthesis of insect-induced volatile terpenes such as ( E )-β-caryophyllene in Arabidopsis thaliana ( Hong et al, 2012 ), ( E )-geraniol and valencene in Citrus sinensis ( Shen et al, 2016 ; Li et al, 2017 ), linalool in Freesia hybrida ( Yang et al, 2020 ), α-farnesene in apple ( Wang et al, 2020 ), ( E )-β-farnesene and ( E )-α-bergamotene in Zea mays ( Li et al, 2015 ). In cotton, GaWRKY1 is involved in the accumulation of δ-cadinene, the essential precursor in the gossypol biosynthetic pathway ( Xu et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Insect-Induced Sesquiterpene Synthase GbTPS1 Based on the Transcriptome of Gossypium barbadense Feeding by Cotton Bollworm

et al. 2022

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When attacked by insect herbivores, plants initiate sophisticated defenses mediated by complex signaling networks and usually release a blend of functional volatiles such as terpenes against infestation. The extra-long staple cotton Gossypium barbadense cultivated worldwide as natural textile fiber crop is frequently exposed to a variety of herbivores, such as cotton bollworm Helicoverpa armigera. However, little is known about insect-induced transcriptional changes and molecular mechanisms underlying subsequent defense responses in G. barbadense. In the current study, transcriptome changes in G. barbadense infested with chewing H. armigera larvae were investigated, and we identified 5,629 differentially expressed genes (DEGs) in the infested cotton leaves compared with non-infested controls. H. armigera feeding triggered complex signaling networks in which almost all (88 out of 90) DEGs associated with the jasmonic acid (JA) pathway were upregulated, highlighting a central role for JA in the defense responses of G. barbadense against target insects. All DEGs involved in growth-related photosynthesis were downregulated, whereas most DEGs associated with defense-related transcript factors and volatile secondary metabolism were upregulated. It was noteworthy that a terpene synthase gene in the transcriptome data, GbTPS1, was strongly expressed in H. armigera-infested G. barbadense leaves. The upregulation of GbTPS1 in qPCR analysis also suggested an important role for GbTPS1 in herbivore-induced cotton defense. In vitro assays showed that recombinant GbTPS1 catalyzed farnesyl pyrophosphate and neryl diphosphate to produce three sesquiterpenes (selinene, α-gurjunene, and β-elemene) and one monoterpene (limonene), respectively. Moreover, these catalytic products of GbTPS1 were significantly elevated in G. barbadense leaves after H. armigera infestation, and elemene and limonene had repellent effects on H. armigera larvae in a dual-choice bioassay and increased larval mortality in a no-choice bioassay. These findings provide a valuable insight into understanding the transcriptional changes reprogramming herbivore-induced sesquiterpene biosynthesis in G. barbadense infested by H. armigera, which help elucidate the molecular mechanisms underlying plant defense against insect pests.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Insect-Induced Sesquiterpene Synthase GbTPS1 Based on the Transcriptome of Gossypium barbadense Feeding by Cotton Bollworm

et al. 2022

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“…A possible functional mechanism for the transcriptional repressor, ERF4 Previous studies have revealed that transcriptional activators often work together to form a synergistic activator complex to co-activate the expression of target genes (Sun et al, 2017;Yao et al, 2017;Wang et al, 2020;Zhang et al, 2020). Similarly, the plant repressor-type ERF TFs could recruit a corepressor to form a transcriptional repressor complex to synergistically block the transcriptional activation of their targets (Song et al, 2005;Zhou et al, 2017).…”

Section: Discussion Erf4 Positively Regulates Plant Growth By Promoti...mentioning

confidence: 99%

ERF4 interacts with and antagonizes TCP15 in regulating endoreduplication and cell growth in Arabidopsis

Ding

Xie³

et al. 2022

JIPB

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Endoreduplication is prevalent during plant growth and development, and is often correlated with large cell and organ size. Despite its prevalence, the transcriptional regulatory mechanisms underlying the transition from mitotic cell division to endoreduplication remain elusive. Here, we characterize ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR 4 (ERF4) as a positive regulator of endoreduplication through its function as a transcriptional repressor. ERF4 was specifically expressed in mature tissues in which the cells were undergoing expansion, but was rarely expressed in young organs. Plants overexpressing ERF4 exhibited much larger

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“…In Artemisia annua , overexpression of AaWRKY1 activates the expression of the key enzyme gene AaCYP71AV1 , thereby promoting the synthesis of artemisinin [ 55 ]. There were also many studies on the transcriptional regulation of bHLH transcription factors on the synthesis of sesquiterpenes, e.g., in Artemisia annua , overexpression of AaMICC2 transcription factors could improve the transcription level of AaCYP71AV1 and DBR2 genes [ 56 ], and in Malus pumila calli, overexpression of MdMYC2 and MdERF3 could significantly increase the transcription levels of MdHMGR2 and MdAFS , thereby increasing the synthesis of α-farneene [ 57 ]. However, it was unclear whether bHLH transcription factors in sandalwood were involved in transcriptional regulation of sandal sesquiterpene biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Analysis of SabHLHs in Santalum album L.

Zhang

Chen

Xiong

et al. 2022

Life

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Santalum album L., a semi-parasitic evergreen tree, contains economically important essential oil, rich in sesquiterpenoids, such as (Z) α- and (Z) β-santalol. However, their transcriptional regulations are not clear. Several studies of other plants have shown that basic-helix-loop-helix (bHLH) transcription factors (TFs) were involved in participating in the biosynthesis of sesquiterpene synthase genes. Herein, bHLH TF genes with similar expression patterns and high expression levels were screened by co-expression analysis, and their full-length ORFs were obtained. These bHLH TFs were named SaMYC1, SaMYC3, SaMYC4, SaMYC5, SabHLH1, SabHLH2, SabHLH3, and SabHLH4. All eight TFs had highly conserved bHLH domains and SaMYC1, SaMYC3, SaMYC4, and SaMYC5, also had highly conserved MYC domains. It was indicated that the eight genes belonged to six subfamilies of the bHLH TF family. Among them, SaMYC1 was found in both the nucleus and the cytoplasm, while SaMYC4 was only localized in the cytoplasm and the remaining six TFs were localized in nucleus. In a yeast one-hybrid experiment, we constructed decoy vectors pAbAi-SSy1G-box, pAbAi-CYP2G-box, pAbAi-CYP3G-box, and pAbAi-CYP4G-box, which had been transformed into yeast. We also constructed pGADT7-SaMYC1 and pGADT7-SabHLH1 capture vectors and transformed them into bait strains. Our results showed that SaMYC1 could bind to the G-box of SaSSy, and the SaCYP736A167 promoter, which SaSSy proved has acted as a key enzyme in the synthesis of santalol sesquiterpenes and SaCYP450 catalyzed the ligation of santalol sesquiterpenes into terpene. We have also constructed pGreenII 62-SK-SaMYC1, pGreenII 0800-LUC-SaSSy and pGreenII 0800-LUC-SaCYP736A167 via dual-luciferase fusion expression vectors and transformed them into Nicotiana benthamiana using an Agrobacterium-mediated method. The results showed that SaMYC1 was successfully combined with SaSSy or SaCYP736A167 promoter and the LUC/REN value was 1.85- or 1.55-fold higher, respectively, than that of the control group. Therefore, we inferred that SaMYC1 could activate both SaSSy and SaCYP736A167 promoters.

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MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple

Cited by 17 publications

References 50 publications

Characterization of a Novel Insect-Induced Sesquiterpene Synthase GbTPS1 Based on the Transcriptome of Gossypium barbadense Feeding by Cotton Bollworm

Characterization of a Novel Insect-Induced Sesquiterpene Synthase GbTPS1 Based on the Transcriptome of Gossypium barbadense Feeding by Cotton Bollworm

ERF4 interacts with and antagonizes TCP15 in regulating endoreduplication and cell growth in Arabidopsis

Identification and Functional Analysis of SabHLHs in Santalum album L.

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