Measles elimination: progress and challenges

Combination of Reverse Transcriptase PCR Analysis and Immunoglobulin M Detection on Filter Paper Blood Samples Allows Diagnostic and Epidemiological Studies of Measles

“…Live attenuated measles virus (MV) vaccines have successfully been used to control measles morbidity and mortality in the industrialized world, but vaccination has been less successful in developing countries. This is thought to be the result of a combination of insufficient vaccination coverage, logistical problems related to cold chain maintenance, civil wars, and safety issues related to the current AIDS pandemic (4,16).…”

Development of an Artificial-Antigen-Presenting-Cell-Based Assay for the Detection of Low-Frequency Virus-Specific CD8+T Cells in Whole Blood, with Application for Measles Virus

“…One hundred microliters of heparinized whole blood was mixed with 400 l PBS containing10 6 peptide aAPCs and incubated at 37°C for 2.5 h while rotating. After incubation, total RNA was extracted using the QIAamp RNA blood minikit (Qiagen GmbH, Hilden, Germany) according to the manufacturers' protocol.…”

“…The live attenuated measles virus (MV) vaccine has eliminated measles in developed countries (2,6,18). Strategies such as mass immunization campaigns conducted by the World Health Organization, UNICEF, and their partners have led to an estimated 60% reduction in measles mortality worldwide relative to the global burden of mortality since 1999 (30).…”

National measles surveillance data analysis, 2005 to 2009, Ethiopia

“…However, the use of measles vaccine over the last 30 years has reduced global measles morbidity and mortality by 74 and 85%, respectively, compared with the pre-vaccine era (Cutts et al, 1999). The World Health Organization (WHO) estimates that almost one million measles-related deaths occur each year, the majority (85%) in Africa and Asia (MMWR, 1999;Altintas et al, 1996).…”