Measurement and Simulation of Permeation and Diffusion in Native and Cultivated Tissue Constructs

Hsu, Hao-Hsiang; Schimek, Katharina; Marx, Uwe; Pörtner, Ralf

doi:10.5772/intechopen.72904

Cited by 6 publications

(9 citation statements)

References 49 publications

(54 reference statements)

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“…Figure 1 shows the schematic of the possible three routes in a skin model. Transcellular routes extract or analyse the ISF directly through the cells [45]. Injecting needles is one of the methods used in this approach.…”

Section: Sampling Methods For Isf Collectionmentioning

confidence: 99%

Microneedle Arrays for Sampling and Sensing Skin Interstitial Fluid

et al. 2021

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Dermal interstitial fluid (ISF) is a novel source of biomarkers that can be considered as an alternative to blood sampling for disease diagnosis and treatment. Nevertheless, in vivo extraction and analysis of ISF are challenging. On the other hand, microneedle (MN) technology can address most of the challenges associated with dermal ISF extraction and is well suited for long-term, continuous ISF monitoring as well as in situ detection. In this review, we first briefly summarise the different dermal ISF collection methods and compare them with MN methods. Next, we elaborate on the design considerations and biocompatibility of MNs. Subsequently, the fabrication technologies of various MNs used for dermal ISF extraction, including solid MNs, hollow MNs, porous MNs, and hydrogel MNs, are thoroughly explained. In addition, different sensing mechanisms of ISF detection are discussed in detail. Subsequently, we identify the challenges and propose the possible solutions associated with ISF extraction. A detailed investigation is provided for the transport and sampling mechanism of ISF in vivo. Also, the current in vitro skin model integrated with the MN arrays is discussed. Finally, future directions to develop a point-of-care (POC) device to sample ISF are proposed.

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Section: Sampling Methods For Isf Collectionmentioning

confidence: 99%

Microneedle Arrays for Sampling and Sensing Skin Interstitial Fluid

et al. 2021

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“…Figure 1 shows the schematic of the possible three routes in a skin model. Transcellular routes extract or analyse the ISF directly through the cells [42]. Injecting needles is one of the ways in this approach.…”

Section: Sampling Methods For Isf Collectionmentioning

confidence: 99%

Microneedle Arrays for Sampling and Sensing Dermal Interstitial Fluid

Kashaninejad¹,

Munaz²,

Moghadas³

et al. 2021

Preprint

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Dermal interstitial fluid (ISF) is a novel source of biomarkers that can be considered as an alternative to blood sampling for disease diagnosis and treatment. Nevertheless, in vivo extraction and analysis of ISF are challenging. On the other hand, microneedle (MN) technology can address most of the challenges associated with dermal ISF extraction and is well-suited for long-term, continuous ISF monitoring as well as in situ detection. In this review, we first briefly summarise the different dermal ISF collection methods and compare them with MN methods. Next, we elaborate on the design considerations and biocompatibility of MNs. Subsequently, the fabrication technologies of various MNs used for dermal ISF extraction, including solid MNs, hollow MNs, porous MNs and hydrogel MNs, are thoroughly explained. In addition, different sensing mechanisms of ISF detection will be discussed in detail. Subsequently, we identify the challenges and propose the possible solutions associated with ISF extraction. A detailed investigation is provided for the transport and sampling mechanism of ISF in vivo. Also, the current in vitro skin model integrated with the MN arrays will be discussed. Finally, future directions to develop a point-of-care (POC) device to sample ISF are proposed.

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“…Therefore, it is quite difficult to determine the timedepending changes of diffusion and permeation. Hsu et al developed a method based on fluorescence measurements to determine and simulate the permeation of high molecular weight compounds through a gel matrix in a multiorgan-chip and applied this to in vivo cultivated skin models [105,112,15].…”

Section: Diffusion/permeability Coefficientsmentioning

confidence: 99%

“…Scaffolds are especially used in more sophisticated bioreactor systems (reviewed in [ 7 ]), which create an appropriate biomimetic microenvironment, recapitulate tissue‐specific cell–cell contacts or cell–matrix interactions, mimic gradients of oxygen, nutrients, waste products or pH, and modulate diffusion of regulatory molecules. One of the major challenges in this regard is controlling the diffusion rates of growth factors, proteins, and other molecules for cell regulation and a careful exchange of nutrients and gases under spatial control [ 13 , 14 , 15 ].…”

Section: Relevant Aspects Of Transport Processes In Tissue Culturesmentioning

confidence: 99%

Generation and evaluation of input values for computational analysis of transport processes within tissue cultures

Fattahi

Taheri

Schilling

et al. 2022

Engineering in Life Sciences

Self Cite

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Techniques for tissue culture have seen significant advances during the last decades and novel 3D cell culture systems have become available. To control their high complexity, experimental techniques and their Digital Twins (modelling and computational tools) are combined to link different variables to process conditions and critical process parameters. This allows a rapid evaluation of the expected product quality. However, the use of mathematical simulation and Digital Twins is critically dependent on the precise description of the problem and correct input parameters. Errors here can lead to dramatically wrong conclusions. The intention of this review is to provide an overview of the state‐of‐the‐art and remaining challenges with respect to generating input values for computational analysis of mass and momentum transport processes within tissue cultures. It gives an overview on relevant aspects of transport processes in tissue cultures as well as modelling and computational tools to tackle these problems. Further focus is on techniques used for the determination of cell‐specific parameters and characterization of culture systems, including sensors for on‐line determination of relevant parameters. In conclusion, tissue culture techniques are well‐established, and modelling tools are technically mature. New sensor technologies are on the way, especially for organ chips. The greatest remaining challenge seems to be the proper addressing and handling of input parameters required for mathematical models. Following Good Modelling Practice approaches when setting up and validating computational models is, therefore, essential to get to better estimations of the interesting complex processes inside organotypic tissue cultures in the future.

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Measurement and Simulation of Permeation and Diffusion in Native and Cultivated Tissue Constructs

Cited by 6 publications

References 49 publications

Microneedle Arrays for Sampling and Sensing Skin Interstitial Fluid

Microneedle Arrays for Sampling and Sensing Skin Interstitial Fluid

Microneedle Arrays for Sampling and Sensing Dermal Interstitial Fluid

Generation and evaluation of input values for computational analysis of transport processes within tissue cultures

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