Background: The Personality Inventory for DSM-5 Brief Form (PID-5-BF) is a 25-item measuring tool evaluating maladaptive personality traits for diagnosis of personality disorders(PDs). As a promising scale, its impressive psychometric properties has been verified in some countries, however, there has no studies about the utility of PID-5-BF in Chinese settings. The current study aimed to examine cultural applicability of the Chinese version of PID-5-BF among undergraduate students and clinical patients.Methods: 7155 undergraduate students and 451 clinical patients completed the Chinese version of PID-5-BF. 228 students were chosen randomly for test-retest reliability at a 4-week interval. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were conducted to discover the most suitable construct in Chinese, measurement invariance(MI), internal consistency and external validity were also calculated. Results: An exploratory six-factor model was supported more suitable in both samples(Undergraduate sample: CFI = 0.905, TLI = 0.888, RMSEA = 0.044, SRMR = 0.039; Clinical sample: CFI = 0.904, TLI = 0.886, RMSEA = 0.047, SRMR = 0.060), adding a new factor“Interpersonal Relationships”. Measurement invariance across non-clinical and clinical sample was established(configural, weak, strong MI, and partial strict MI). Aside from acceptable internal consistency(Undergraduate sample: alpha=0.84, MIC=0.21; Clinical sample: alpha=0.86, MIC=0.19) and test-retest reliability(0.73), the association with 220-item PID-5 was significant(r = 0.93, p < 0.01), and six PDs measured by Personality diagnostic questionnaire-4+ (PDQ-4+) was correlated with expected domains of PID-5-BF. Conclusions: The Chinese version of the PID-5-BF showed satisfactory psychometric properties, which is a convenient and useful screening tool for personality disorders.