Measurement of 5-Androsten-3&lt;i&gt;&amp;beta;&lt;/i&gt;, 17&lt;i&gt;&amp;beta;&lt;/i&gt;-diol in Spermatic and Peripheral Venous Blood Samples from the Same Human Subjects by a Radioimmunoassay Method

Forti, Gianni; Calabresi, E.; Giannotti, P.; Borrelli, D; Gonnelli, P; Barbieri, U; Serio, Mario

doi:10.1159/000178913

Cited by 11 publications

(2 citation statements)

References 11 publications

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“…Reference intervals for adiol in serum are shown in Table 1. These GC-MS results are in good agreement with those obtained in age and sex matched groups by competitive protein binding assays (Rosenfield & Otto, 1972;Demisch et al, 1973) and radioimmunoassay (Forti et al, 1978;Wang et al, 1979;Bonney et al, 1983b) They are however slightly lower than the results obtained by gas chromatography (Sirinathsinghji & Mills, 1985). Reference intervals for adiol-3s are also shown in Table 1.…”

Section: Patients and Controlssupporting

confidence: 84%

Delta‐5‐androstenediol and its sulphate in serum and urine of normal adults and patients with endocrine diseases

Dikkeschei¹,

Willemse

Wolthers³

et al. 1993

Clinical Endocrinology

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The wide concentration range of adiol and adiol-3S in urine makes analysis of these steroids in urine of little clinical value. Serum concentrations of adiol and adiol-3S are higher in men than in women. Premenopausal values are higher than post-menopausal. Adiol and adiol-3S in serum are significantly correlated in pre and post-menopausal women, r = 0.51 and r = 0.69 respectively, but not in men. In endocrine patients the serum concentrations of adiol show an ACTH or LH dependency in women; adiol correlates with cortisol, dehydroepiandrosterone or androstenedione and, in males, additionally with testosterone. However, in several situations adiol correlates with none of these steroids. Although adiol secretion can be stimulated by ACTH and LH, the level of serum adiol is also determined by other factors. Finally, in adrenal carcinoma serum adiol and adiol-3S may be used as tumour markers.

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Section: Patients and Controlssupporting

confidence: 84%

Delta‐5‐androstenediol and its sulphate in serum and urine of normal adults and patients with endocrine diseases

Dikkeschei¹,

Willemse

Wolthers³

et al. 1993

Clinical Endocrinology

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“…(1) seemed to be reduced owing to crossreaction of 13% with androst-5-ene-3j3,17/3-diol, which has been estimated in male plasma in rather high concentrations ranging from 2.28 to 5.47 nmol/1 depending upon the specificity of the antiserum and the method of separation used (9,10,11,12,13). Unfortunately, the removal of androst-5-ene-3/3,17/3-diol by epoxidation (2) gave good results only with pure steroids; but introduced unknown factors which inhibited the displacement reaction with plasma extracts.…”

Section: Plasma Concentrationsmentioning

confidence: 99%

Unconjugated 5α-Androstan-3α,17ß-diol and 5α-Androstane-3ß,17ß-diol in Human Plasma as Measured by Radioimmunoassay without Chromatography

Tamm¹,

Volkwein²

1981

CCLM

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Summary: A radioimmunoassay (RIA) without chromatography for the determination of 5a-androstan-3a,17j3-diol and of 5a-androstane-3(3,17/3-diol in human plasma by using highly specific antisera against the 15/3-carboxyethylmercapto-bovine serum albumin conjugates is described. It could be shown that the 13% cross reaction of the 5a-androstane-3/3,17/3-diol antiserum with pure androst-5-ene-3/3,17/3-diol was negligibly low in plasma extracts. Sensivity, accuracy, precision, and linearity revealed results which comply with the requisites of a reliable RIA. The plasma levels obtained were as follows (mean ± SD): 5a-androstan-3a, 17/3-diol: Normal males (N = 27) 0.86 ± 0.22 nmol/1; normal females (N = 10) 0.31 ± 0.07 nmol/1; hirsute females (N = 25) 0.60 ± 0.24 nmol/1; 5a-androstane-3/3,170-diol: Normal males 1.47 ± 0.43 nmol/1; normal females 0.49 ±0.10 nmol/1; hirsute females 0.91 ± 0.32 nmol/1. Unkonjugiertes 5a-Androstan-3a, l Jfrdiol und 5a-Androstan-3ß, 17ß-diol im Plasma des Menschen, mit Radioimmunoassay ohne Chromatographie bestimmtZusammenfassung: Es wird ein Radioimmunoassay ohne Chromatographie zur Bestimmung von 5a-Androstan-3a, 17j3-diol und 5a-Androstan-3/3,17/3-diol im menschlichen Plasma beschrieben. Er basiert auf hoch-spezifischen Antisera gegen die ISß-Carboxyethylmercapto-Rinderserumalbumin-Konjugate dieser Steroide. Die 13 %ige Kreuzreaktion des 5a-Androstan-3/3,17j3-diol-Antiserums gegen reines Androst-5-en-3/3,17/3-diol war extrem niedrig mit Plasma-Extrakten. Empfindlichkeit, Richtigkeit, Genauigkeit und Linearität entsprechen den Kriterien eines zuverlässigen RIA. Die gemessenen Plasma^Konzentrationen waren folgende ( ± s): 5a-Androstan-3a, 17/3-diol: Normale Männer (N = 27) 0,86 ± 0,22 nrnol/l; normale Frauen (N = 10) 0,31 ± 0,07 nmol/1; hirsute Frauen (N = 25) 0,60 ± 0,24 nmol/1; 5a-Androstan-3/3, 17/3-diol: Normale Männer 1,47 ± 0,43 nmol/1; normale Frauen 0,49 ±0,10 nmol/1; hirsute Frauen 0,91 ± 0,32 nmol/1.

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Immunochemical Methods for Adrenal and Gonadal Steroids

Pazzagli¹,

Serio²

1987

Radioimmunoassay in Basic and Clinical Pharmacology

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Measurement of 5-Androsten-3<i>β</i>, 17<i>β</i>-diol in Spermatic and Peripheral Venous Blood Samples from the Same Human Subjects by a Radioimmunoassay Method

Cited by 11 publications

References 11 publications

Delta‐5‐androstenediol and its sulphate in serum and urine of normal adults and patients with endocrine diseases

Delta‐5‐androstenediol and its sulphate in serum and urine of normal adults and patients with endocrine diseases

Unconjugated 5α-Androstan-3α,17ß-diol and 5α-Androstane-3ß,17ß-diol in Human Plasma as Measured by Radioimmunoassay without Chromatography

Immunochemical Methods for Adrenal and Gonadal Steroids

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