Measurement of Adenosine 3′,5′-Cyclic Monophosphate and Guanosine 3′,5′-Cyclic Monophosphate in Mussel (Mytilus galloprovincialis Lmk.) by High-Performance Liquid Chromatography with Diode Array Detection

Enrich, M.; Villamarı́n, J.Antonio; Martinez, Juan Ignacio Ramos; Ibarguren, Izaskun

doi:10.1006/abio.2000.4731

Cited by 21 publications

(11 citation statements)

References 27 publications

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“…Extraction and detection of cGMP from C. sinensis pollen tubes were performed as previously described with slight modifications [30]. Briefly, 0.5 g pollen grains were incubated for 2 h, the pollen tubes were harvested, and quickly homogenized in an ice bath, after adding cooled 0.6 M PCA.…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide Participates in Cold-Inhibited Camellia sinensis Pollen Germination and Tube Growth Partly via cGMP In Vitro

Wang

Zhuge

et al. 2012

PLoS ONE

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Nitric oxide (NO) plays essential roles in many biotic and abiotic stresses in plant development procedures, including pollen tube growth. Here, effects of NO on cold stress inhibited pollen germination and tube growth in Camellia sinensis were investigated in vitro. The NO production, NO synthase (NOS)-like activity, cGMP content and proline (Pro) accumulation upon treatment with NO scavenger cPTIO, NOS inhibitor L-NNA, NO donor DEA NONOate, guanylate cyclase (GC) inhibitor ODQ or phosphodiesterase (PDE) inhibitor Viagra at 25°C (control) or 4°C were analyzed. Exposure to 4°C for 2 h reduced pollen germination and tube growth along with increase of NOS-like activity, NO production and cGMP content in pollen tubes. DEA NONOate treatment inhibited pollen germination and tube growth in a dose-dependent manner under control and reinforced the inhibition under cold stress, during which NO production and cGMP content promoted in pollen tubes. L-NNA and cPTIO markedly reduced the generation of NO induced by cold or NO donor along with partly reverse of cold- or NO donor-inhibited pollen germination and tube growth. Furthermore, ODQ reduced the cGMP content under cold stress and NO donor treatment in pollen tubes. Meanwhile, ODQ disrupted the reinforcement of NO donor on the inhibition of pollen germination and tube growth under cold condition. Additionally, Pro accumulation of pollen tubes was reduced by ODQ compared with that receiving NO donor under cold or control condition. Effects of cPTIO and L-NNA in improving cold-treated pollen germination and pollen tube growth could be lowered by Viagra. Moreover, the inhibitory effects of cPTIO and L-NNA on Pro accumulation were partly reversed by Viagra. These data suggest that NO production from NOS-like enzyme reaction decreased the cold-responsive pollen germination, inhibited tube growth and reduced Pro accumulation, partly via cGMP signaling pathway in C. sinensis.

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Section: Methodsmentioning

confidence: 99%

Nitric Oxide Participates in Cold-Inhibited Camellia sinensis Pollen Germination and Tube Growth Partly via cGMP In Vitro

Wang

Zhuge

et al. 2012

PLoS ONE

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“…They are applicable on estimation of cyclic and others nucleotides. The nucleotides are recognized and separated according to their charge and hydrophobicity, if RP-18 serves as stacionary phase and phosphate buffer with tetrabutylammonium hydroxide or ammonium acetate as ion-pairing agent [91,92,60]. These methods usually require pre-purification of biological sample (e.g.…”

Section: Methods Based On Hplcmentioning

confidence: 99%

“…There are published also several works of utilizing HPLC to cGMP estimation based on molecular recognition on stationary phase [60,106,62].…”

Section: Miscellaneous Methodsmentioning

confidence: 99%

Molecular Recognition of Cyclic-Nucleotides and Current Sensors for Their Detection

Šebestı́k¹,

Hlaváček²,

Stibor

2005

CPPS

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This work briefly describes available sensors for cAMP and cGMP. Many sensors are based on derivatization of naturally occurring products such as immunoglobulins, protein kinases, etc. Only a few published works deal with chemosensors, which are built up by "total" chemical synthesis. This field stays opened for combinatorial chemist. The best sensors are protein kinases genetically modified with mutants of green fluorescent protein, which allow screening of entire cell cultures.

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“…The need for a cyclic nucleotide assay, which has the high degree of sensitivity and precision of the radioimmunoassay, without the complications associated with the use of radioactive isotopes, has led to the development of a range of non-isotopic assays using high performance liquid chromatography (10), fluorescent indicators (11), mass spectrometry (12) and non-isotopic immunoassays based on the enzyme-linked immunosorbent assay (ELISA) method first reported by Engvall et al (13). The quantification of cellular antigens by the ELISA method is comparable to the RIA in sensitivity, reproducibility and application in all cases where a direct comparison between RIA and ELISA has been executed (14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

An Enzyme-Linked Immunosorbent Assay for the Rapid Quantification of Intracellular and Extracellular Guanosine 3?,5?-cyclic Monophosphate in Cultured Glial Cells

2004

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A sensitive enzyme-linked, indirect immunosorbent assay (ELISA) for the determination of guanosine 3',5'-cyclic monophosphate (cGMP) in glial cells is described. The assay uses an antiserum produced against succinylated cGMP and is based on the competition between endogenous cGMP and a fixed amount of immobilized antigen. The antibody exhibited a high degree of specificity with negligible cross reactivity with other nucleotides or related compounds. The standard curve, linearized using a logit-log transformation, had an operating range of 1 fmol/50 microl to 5 pmol/50 microl. The sensitivity of the assay was significantly increased by acetylation of standards and samples. Recoveries of cGMP from samples of cultured cells ranged from 85% to 105% with a mean recovery of 97% +/- 7%. Levels of cGMP measured with the ELISA were in agreement with the corresponding values obtained using radioimmunoassay. The present method provides for a cheap, sensitive and simple alternative to the commercially available cGMP assays.

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Measurement of Adenosine 3′,5′-Cyclic Monophosphate and Guanosine 3′,5′-Cyclic Monophosphate in Mussel (Mytilus galloprovincialis Lmk.) by High-Performance Liquid Chromatography with Diode Array Detection

Cited by 21 publications

References 27 publications

Nitric Oxide Participates in Cold-Inhibited Camellia sinensis Pollen Germination and Tube Growth Partly via cGMP In Vitro

Nitric Oxide Participates in Cold-Inhibited Camellia sinensis Pollen Germination and Tube Growth Partly via cGMP In Vitro

Molecular Recognition of Cyclic-Nucleotides and Current Sensors for Their Detection

An Enzyme-Linked Immunosorbent Assay for the Rapid Quantification of Intracellular and Extracellular Guanosine 3?,5?-cyclic Monophosphate in Cultured Glial Cells

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