Measurement of Aminotransferases: Part 1. Aspartate Aminotransferase

Rej, Robert

doi:10.3109/10408368409167137

Cited by 63 publications

(19 citation statements)

References 617 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…46 Blood of patients with myocardial infarction show release of mitochondrial proteins. [47][48][49] Our data shed new light on this release and indicate that two effects may result: a loss of ATP synthetic capacity and a potential alteration in the myofilaments as these products associate with the contractile proteins. Substantiation of this latter speculation requires systematic investigation of the effects of the ATP synthase proteins on myofilament activity and regulation.…”

Section: Van Eyk Et Almentioning

confidence: 99%

Breakdown and Release of Myofilament Proteins During Ischemia and Ischemia/Reperfusion in Rat Hearts

Eyk

Powers²,

Law³

et al. 1998

Circulation Research

213

156

View full text Add to dashboard Cite

Abstract-Our objective in experiments reported here was to identify myofilament proteins of rat hearts either lost or degraded by cardiac ischemia (15-or 60-minute duration) with and without 45 minutes of reperfusion. We correlated these changes with alterations in myofilament sensitivity to Ca 2ϩ and maximum force generation. Protein degradation and loss were assessed by high-performance liquid chromatography, SDS-PAGE, Western blotting analysis, and amino acid sequencing. Compared with nonischemic control hearts, bundles of skinned fibers from hearts subjected to ischemia alone demonstrated a decrease in maximum force generation and an increase in sensitivity to Ca 2ϩ . These changes in function were increased with the duration of the ischemia and with reperfusion. With increasing duration of ischemia, there was an increased loss and degradation of myofibrillar ␣-actinin and troponin I (TnI) at its C-terminus. ␣-Actinin and TnI were most susceptible to ischemia, but with 60 minutes of ischemia/reperfusion, there was also degradation of myosin light chain-1 (MLC1) involving a clip of residues 1 to 19. The MLC1 degradation product was detected in the reperfusion effluent (along with troponin T, tropomyosin, and ␣-actinin) but not in the tissue with 60 minutes of ischemia with no reperfusion. Moreover, with ischemia the following proteins became associated with the myofibrils: GAPDH and proteins of the mitochondrial ATP synthase complex. Our results provide new evidence regarding the mechanism by which ischemia/reperfusion causes myocardial injury and support the hypothesis that an important element in the injury is altered activity and structure of the myofilaments. (Circ Res. 1998;82:261-271.)

show abstract

Section: Van Eyk Et Almentioning

confidence: 99%

Breakdown and Release of Myofilament Proteins During Ischemia and Ischemia/Reperfusion in Rat Hearts

Eyk

Powers²,

Law³

et al. 1998

Circulation Research

213

156

View full text Add to dashboard Cite

show abstract

“…Three to five subforms of c-AST have been identified by isoelectric focusing, while only one form of m-AST has been found [5,6]. The molecular weight of the holoenzymes isolated from human liver was determined to be 93,000 for c-AST and 90,400 for m-AST [7]. Other biochemical and structural differences of the two enzymes are summarized in Table 5.1.…”

Section: Molecular Formsmentioning

confidence: 99%

5. Aspartate aminotransferase and alanine aminotransferase

El-Khoury¹,

Wang²

2014

Diagnostic Enzymology

View full text Add to dashboard Cite

A 59-year-old female presented to the headache center with complaints of recurring headaches over the past few months. She had not been sleeping well, her headaches were worsening, and she was experiencing frequent hot flashes. Laboratory studies were unremarkable except for an elevated aspartate aminotransferase (AST) value of 175 U/L. The patient drank alcohol occasionally and exercised frequently. The physician requested that the test be repeated in 2 weeks to confirm the abnormal result and instructed the patient to avoid alcohol use and strenuous exercise during that period. Repeat testing 2 weeks later showed an AST of 164 U/L along with the following results: hepatitis A total antibody, positive; hepatitis A IgM antibody, negative; hepatitis C antibody, negative; iron, 17.4 μmol/L; total iron binding capacity, 48 μmol/L; and transferrin saturation, 36%. The patient was known to have immunity to hepatitis B. The persistently elevated AST was suspected to be caused by certain medications that she was taking. Testing was repeated in another 2 weeks along with a hepatic function panel and showed the following results: activated partial thromboplastin time (APTT), 30.5 s; prothrombin time (PT), 10.8 s; γ-glutamyltransferase (GGT), 15 U/L; albumin, 42 g/L; total bilirubin, 5.

show abstract

“…ALT activity in liver is about (3,000 times) serum activities [16]. ALT is exclusively cytoplasmic [17]. The half-life of total ALT is (47 10 hours) [18].…”

Section: Introductionmentioning

confidence: 99%

Serum Levels of 2microglobulin and Some Biochemical Parameters Among Chronic Hepatitis B Patients

Al-Shaikle¹

2010

JNUS

View full text Add to dashboard Cite

The aim of this study was to estimate the level of 2 microglobulin (B2M), total serum bilirubin (TSB) and alanine aminotransferase (ALT) in the sera of (30) chronic hepatitis B (CHB) patients (patients group) and (30) healthy HBV carriers as (control group). The study showed that levels of 2 microglobulin were high among patients group (3.41 1 mg/L), while it was normal among control group (1.42 0.35 mg/L). Regarding biochemical parameters, it was found that (TSB) and (ALT) were increased among patients group, and their level were (37.66 30.8 mol/L) and (43.6 32.51 U/L) respectively. While it was normal among control group (4 0 mol/L) and (17.4 7.16 U/L) respectively.

show abstract

Measurement of Aminotransferases: Part 1. Aspartate Aminotransferase

Cited by 63 publications

References 617 publications

Breakdown and Release of Myofilament Proteins During Ischemia and Ischemia/Reperfusion in Rat Hearts

Breakdown and Release of Myofilament Proteins During Ischemia and Ischemia/Reperfusion in Rat Hearts

5. Aspartate aminotransferase and alanine aminotransferase

Serum Levels of 2microglobulin and Some Biochemical Parameters Among Chronic Hepatitis B Patients

Contact Info

Product

Resources

About