2010
DOI: 10.1016/j.jneumeth.2010.01.018
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Measurement of anti-Aβ1–42 antibodies in intravenous immunoglobulin with indirect ELISA: The problem of nonspecific binding

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Cited by 34 publications
(30 citation statements)
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“…Treatment with Aβ antibodies were beneficial for AD patient by correction of defective immune system [43]. The Aβ antibodies could prevent A β neurotoxicity [26, 27], inhibit Aβ oligomer formation [17, 28, 37] and increase phagocytic clearance of Aβ [29]. However, Aβ autoantibodies have also been associated with angiopathy in brain in some AD patients who has been treated with the IV autoantidodies (44).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Treatment with Aβ antibodies were beneficial for AD patient by correction of defective immune system [43]. The Aβ antibodies could prevent A β neurotoxicity [26, 27], inhibit Aβ oligomer formation [17, 28, 37] and increase phagocytic clearance of Aβ [29]. However, Aβ autoantibodies have also been associated with angiopathy in brain in some AD patients who has been treated with the IV autoantidodies (44).…”
Section: Discussionmentioning
confidence: 99%
“…The results of these studies have been inconsistent: some reported that Aβ auto-antibodies in AD were lower than in normal subjects [7, 8, 9, 10], some unaltered [11, 12, 13], and some increased [14, 15, 16]. The inconsistent results may have been caused by several factors including nonspecific bindings [17], serum Aβ interference [18], incorrect diagnosis [19, 20], structural conformation of Aβ1-42, and/or small sample size. Despite the relatively large number of studies being conducted, the epitope-specific binding and isotyping of the auto-antibodies against Aβ1-42 have not been reported.…”
Section: Introductionmentioning
confidence: 99%
“…standard ELISA is performed under conditions of high ligand immobilization where minimal IgG analyte remains equilibrated in solution. Second, natural human IgG preparations contain very high background signal due to polyvalent antibodies [8,14]. Third, MAb peptides have been shown to adopt OAb and FAb conformations when immobilized on ELISA plates and affinity column matrices [11].…”
Section: Introductionmentioning
confidence: 99%
“…Based on this success, SPR may also prove to be a good method to perform more challenging binding kinetic measurements between natural Ab + IgG and aggregated Ab states [12,16]. In contrast to ELISA experiments, SPR measurements showed minimal binding from polyvalent IgG in IVIG [8,10,14]. Natural Ab + IgG does not appear to bind MAb in SPR studies, in contrast to inconsistent findings of Ab + IgG binding with MAb in ELISA studies [8][9][10][11]17,18].…”
Section: Introductionmentioning
confidence: 99%
“…Conversely, subsequent studies reported equal or increased titers of circulating anti-Aβ antibodies in untreated AD patients based on ELISAs performed on immunoglobulin preparations treated at low pH to dissociate antigen from antibody [11]. However, it has been reported that exposure of low -affinity polyclonal IgG to low pH may result in structural changes or even partial denaturation of antibodies which may generate artefactual increases in apparent activity against endogenous antigens [15, 16]. In vitro assays such as ELISAs employing IgG isolated from human plasma using methods that require a treatment at low pH may therefore artefactually under- or over- estimate IVIG activity against endogenous antigens [1719].…”
Section: Introductionmentioning
confidence: 99%