Measurement of anti-double-stranded DNA antibodies in major immunoglobulin classes

Aotsuka, S; Okawa, Masako; Ikebe, K; Yokohari, R

doi:10.1016/0022-1759(79)90337-5

Cited by 86 publications

(28 citation statements)

References 19 publications

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“…Classifications that merely state that the disease was active if 1 or 2 systems were "involved" (24)(25)(26)(27)(28)(29) can have serious drawbacks. Initially, the University College Hospital (UCH) Middlesex lupus study group categorized the disease as inactive, mild, or severely active (13).…”

Section: Discussionmentioning

confidence: 99%

Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus

Isenberg

Shoenfeld

Schwartz

1984

Arthritis & Rheumatism

106

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Section: Discussionmentioning

confidence: 99%

Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus

Isenberg

Shoenfeld

Schwartz

1984

Arthritis & Rheumatism

106

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“…Two different antigen-specific systems, namely DNA as autoantigen and KLH as exogenous antigen, were used. For anti-DNA antibody, 100 p I of poly-L-lysine (Sigma) was added to a microtiter plate (Cook Engineering) to facilitate the binding of DNA (at 37°C for 2 hours) (20).…”

Section: Amounts Ofmentioning

confidence: 99%

Cellular mechanism of DNA‐specific antibody synthesis by lymphocytes from systemic lupus erythematosus patients

Takeuchi

Abe

Koide

et al. 1984

Arthritis & Rheumatism

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The cellular mechanism of anti-DNA antibody synthesis in patients with systemic lupus erythematosus (SLE) was studied by DNA-specific solid-phase radioimmunoassay . Anti-DNA antibody synthesis in response to DNA was T-dependent, and the experiments with reconstituted lymphocytes from identical twins discordant for SLE showed that B cells and T cells from SLE patients must cooperate to synthesize anti-DNA antibody. Systemic lupus erythematosus (SLE) is a disease of unknown etiology, characterized by multisystem involvement. Numerous immunologic abnormalities have been reported, including hypergammaglobulinemia and production of autoantibodies. The autoantibodies are primarily in the form of immune complexes, closely associated with the pathogenesis of SLE (1,2). DNNanti-DNA antibody immune complexes have been detected in sera and kidneys of SLE patients and have a highly significant association with lupus nephritis (3-8). These observations demonstrate the importance of elucidating the mechanism of anti-DNA antibody synthesis, to achieve a better understanding of the pathogenesis of SLE.We and others have previously reported the loss of suppressor T cell function and the presence of antilymphocyte antibody which preferentially reacts with suppressor T cells, as a possible cause of the immunologic abnormalities in SLE (9-16). In those experiments, nonspecific mitogens such as pokeweed mitogen (PWM) and concanavalin A (Con A) were used to determine the immunologic abnormalities. However, the mechanism of in vitro anti-DNA antibody synthesis and the role of regulatory T cells in response to DNA remained to be clarified. We therefore undertook to investigate the mechanism of in vitro anti-DNA antibody synthesis in response to DNA in SLE patients. MATERIALS AND METHODSSubjects. Twenty-one SLE patients seen at Keio University Hospital were examined. Eight patients were in the active stage and 13 in the inactive stage.

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“…Several investigators have attached DNA to untreated plastics (22)(23)(24)(25)(26)(27), although some reports have claimed that nDNA is not adsorbed to untreated polystyrene or polyvinyl (34,35). Pretreatment of plastics with poly-L lysine for radioimmunoassays (3 1.32) or protamine sulfate for EIA (25) has been successfully tried.…”

Section: Discussronmentioning

confidence: 99%

Enzyme immunoassay for antibodies to native DNA

Eaton

Schnneider

Schur

1983

Arthritis & Rheumatism

109

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An enzyme immunoassay was developed to detect antibodies to native DNA; DNA coating conditions that maximized sensitivity, specificity, and reproducibility were selected. Sera of patients with systemic lupus erythematosus (SLE) were positive more frequently by this immunoassay than by the Crithidiu luciliae assay or by counterimmunoelectrophoresis. By enzyme immunoassay, 94% of sera with active SLE and 70% of sera from patients with inactive SLE were positive, as were 16% from those suspected of having SLE, and 2.5% of normal persons. Specificity for native DNA was shown for both SLE and normal sera by inhibition studies and by S1 nuclease treatment of polystyrene-bound native DNA. The enzyme immunoassay correlated more with serum hemolytic complement levels than did the other 2 assays, suggesting that it detects biologically more relevant anti-DNA antibodies than do the other 2 tests.Measurement of serum anti-DNA antibody levels has been helpful in both the diagnosis and clinical assessment of systemic lupus erythematosus (SLE) detect antibodies to native DNA (nDNA), including immunodiffusion (7), complement fixation (8,9), agglutination (lO,ll), immunofluorescence (6,12), and radioimmunoassays (13-18). The sensitivity, specificity, cost, and ease of performance of these assays vary considerably (19,20).(The enzyme immunoassay (EIA) appears to offer advantages over other assays in areas of sensitivity, reproducibility, and efficiency, without sacrificing specificity. Since the initial description by Engvall and Perlmann (21), many antigens have been bound to solid phases to detect antibodies by EIA. Most of these antigens have been proteins or polysaccharides, but the binding of nucleic acids to polystyrene has not been systematically studied. Recently, several papers have appeared describing the application of EIAs to detect antibodies to DNA (22-29). In each of these reports different conditions were used, and the success of binding nDNA to the solid phase has varied (23,25).The purpose of the present study was to develop an EIA that could detect antibodies to nDNA, including a reproducible and reliable method for binding of DNA to the solid phase; this EIA was to be both sensitive and specific. In addition, the antibodies detected by the EIA were compared with those detected by other tests to define the (biologic) quality and relevance of the antibodies analyzed by different techniques. MATERIALS AND METHODSSource of sera. Normal human sera were obtained either from hospital personnel (sera kindly provided by Mr.

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Measurement of anti-double-stranded DNA antibodies in major immunoglobulin classes

Cited by 86 publications

References 19 publications

Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus

Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus

Cellular mechanism of DNA‐specific antibody synthesis by lymphocytes from systemic lupus erythematosus patients

Enzyme immunoassay for antibodies to native DNA

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