Measurement of antioxidative activity against hypochlorite ion by sequential injection analysis with luminol chemiluminescence detection

A novel flow-injection analysis system for evaluation of antioxidants by using sodium dichloroisocyanurate as a source of hypochlorite anion

“…Subsequently, a dose-response curve for each test compound was drawn by plotting the fluorescence peak height at each concentration of test compound against the concentration of the test compound (n = 5). The concentration of the test compound indicating 50% of the blank value was regarded as the EC 50 of the compound according to previous papers (23,24). Thus, in the present study, the EC 50 value (μM) was evaluated as the anti-oxidant activity of the test compound.…”

Development of an Evaluation Method for Hydroxyl Radical Scavenging Activities Using Sequential Injection Analysis with Chemiluminescence Detection

“…[13][14][15] As mentioned above, ·OH is regarded as the most reactive species of ROS, and as such, ·OH scavenger should be effective to protect from serious oxidative stress caused by ·OH. Therefore, in this research, we developed the SIA-CL detection method to detect ·OH for the evaluation of ·OH scavenging activity.…”

Automated analysis of the serum antioxidative activities against five different reactive oxygen species by sequential injection system with a chemiluminescence detector

“…In this method, the attenuation of luminol CL due to scavenging of ROS by sample was measured as the antioxidative activity. Successively, luminol CL detection was hyphenated with 7 sequential injection analysis (SIA) for determination of antioxidative activities against ClO -, 1 O 2 , O 2 -and NO [14][15][16]. Employing SIA makes it possible to reduce sample volume, reagent consumption and human error and obtain repeatable results, comparing with flow injection analysis [17].…”