Measurement of Biomarkers of Oxidative Stress and Airway Inflammation in Exhaled Breath Condensate: Methodology and Potential Applications in Patients with COPD and Healthy Smokers

Montuschi, Paolo

doi:10.1016/b978-0-44-462613-4.00019-2

Cited by 7 publications

(8 citation statements)

References 83 publications

(109 reference statements)

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“…Biomarkers of nucleic acid oxidation are 8-hydroxyguanosine and 8-hydroxy-2′-deoxyguanosine. High concentrations of the biomarkers were determined not only in body fluids or tissues of patients with age-related and/or degenerative diseases such as Alzheimer's disease, hypertension, type II diabetes, or several types of cancer (see Table 1 for the summary of diseases and detected biomarkers) but also in relation to chronic obstructive pulmonary disease [ 10 ], smoking [ 11 , 12 ], and air pollution [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Multimarker Screening of Oxidative Stress in Aging

Syslová

Böhmová

Mikoška

et al. 2014

Oxidative Medicine and Cellular Longevity

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Aging is a complex process of organism decline in physiological functions. There is no clear theory explaining this phenomenon, but the most accepted one is the oxidative stress theory of aging. Biomarkers of oxidative stress, substances, which are formed during oxidative damage of phospholipids, proteins, and nucleic acids, are present in body fluids of diseased people as well as the healthy ones (in a physiological concentration). 8-iso prostaglandin F2α is the most prominent biomarker of phospholipid oxidative damage, o-tyrosine, 3-chlorotyrosine, and 3-nitrotyrosine are biomarkers of protein oxidative damage, and 8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine are biomarkers of oxidative damage of nucleic acids. It is thought that the concentration of biomarkers increases as the age of people increases. However, the concentration of biomarkers in body fluids is very low and, therefore, it is necessary to use a sensitive analytical method. A combination of HPLC and MS was chosen to determine biomarker concentration in three groups of healthy people of a different age (twenty, forty, and sixty years) in order to find a difference among the groups.

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Section: Introductionmentioning

confidence: 99%

Multimarker Screening of Oxidative Stress in Aging

Syslová

Böhmová

Mikoška

et al. 2014

Oxidative Medicine and Cellular Longevity

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“…8-isoprostane F2-α is one of the oxidative stress and lipid peroxidation markers, which is stably formed via non-enzymatic peroxidation of fatty acids, primarily arachidonic acid [ 59 ]. Figure 5 shows the 8-isoprostane F2-α and TNF-α levels of SW1353 chondrocytes after 24 h MIA, H 2 O 2 and IL-1β treatment.…”

Section: Resultsmentioning

confidence: 99%

Establishing SW1353 Chondrocytes as a Cellular Model of Chondrolysis

Pang

Chow

Leong

et al. 2021

Life

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Osteoarthritis (OA) is the most common degenerative joint disease characterised by chondrocyte cell death. An in vitro model of chondrocyte cell death may facilitate drug discovery in OA management. In this study, the cytotoxicity and mode of cell death of SW1353 chondrocytes treated with 24 h of OA inducers, including interleukin-1β (IL-1β), hydrogen peroxide (H2O2) and monosodium iodoacetate (MIA), were investigated. The microscopic features, oxidative (isoprostane) and inflammatory markers (tumour necrosis factor-alpha; TNF-α) for control and treated cells were compared. Our results showed that 24 h of H2O2 and MIA caused oxidative stress and a concentration-dependent reduction of SW1353 cell viability without TNF-α level upregulation. H2O2 primarily induced chondrocyte apoptosis with the detection of blebbing formation, cell shrinkage and cellular debris. MIA induced S-phase arrest on chondrocytes with a reduced number of attached cells but without significant cell death. On the other hand, 24 h of IL-1β did not affect the cell morphology and viability of SW1353 cells, with a significant increase in intracellular TNF-α levels without inducing oxidative stress. In conclusion, each OA inducer exerts differential effects on SW1353 chondrocyte cell fate. IL-1β is suitable in the inflammatory study but not for chondrocyte cell death. H2O2 and MIA are suitable for inducing chondrocyte cell death and growth arrest, respectively.

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“…The concentration of 8-isoprostane and H 2 O 2 present elevation in the exhaled breath condensate of patients with COPD 29 . This evidence supports the existence of oxidative stress, which is responsible for the damage especially of the lower respiratory tract.…”

Section: Inflammation and Dyspnea In Copdmentioning

confidence: 93%

Exhaled breath condensate: a potential tool to evaluate the relationship between inflammation and dyspnea in patients with chronic obstructive pulmonary disease

Patsiris¹,

Stelios²,

Papanikolaou³

et al. 2021

J Lung Health Dis

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Chronic obstructive pulmonary disease (COPD) is a respiratory disease with high prevalence. Many factors contribute to its development, and probably that leads to its various clinical pictures. Inflammation is the mechanism responsible for the structural alterations in the lungs. Despite its heterogeneity, there are a couple of primary symptoms characterizing it, which are chronic and productive cough and dyspnea. The understanding of dyspnea in COPD is based on theories deriving from the interaction of a network formed between the cardiorespiratory and the neuromuscular system and their receptors. Many factors contribute to its occurrence, making it complex and giving it a very subjective character for a person to perceive. Various methods are used to study COPD. Non-invasive ones seem to attract attention nowadays. One of them is the exhaled breath condensate. It is a biofluid with rich content, which can capture a picture of the pathological processes happening in the lungs. Its study has shown that some markers of inflammation and oxidative stress, such as 8-isoprostane and H2O2, are elevated and able to connect dyspnea and inflammation. Additionally, they seem to provide information of the ongoing inflammatory process in the lungs as well as a picture of the severity of the symptoms. This evidence may enhance the association of dyspnea with dysfunctional breathing. Despite these interesting findings, further research is necessary both in dyspnea and inflammation in COPD to clarify their mechanisms and connective pathways. The utility of non-invasive techniques such as the exhaled breath condensate could be of significant help, but its establishment in the medical field requires extra studies.

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Measurement of Biomarkers of Oxidative Stress and Airway Inflammation in Exhaled Breath Condensate: Methodology and Potential Applications in Patients with COPD and Healthy Smokers

Cited by 7 publications

References 83 publications

Multimarker Screening of Oxidative Stress in Aging

Multimarker Screening of Oxidative Stress in Aging

Establishing SW1353 Chondrocytes as a Cellular Model of Chondrolysis

Exhaled breath condensate: a potential tool to evaluate the relationship between inflammation and dyspnea in patients with chronic obstructive pulmonary disease

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