Measurement of blast wave by a miniature fiber optic pressure transducer in the rat brain

Chavko, M; Koller, Wayne A.; Prusaczyk, W. K.; McCarron, Richard M.

doi:10.1016/j.jneumeth.2006.07.018

Cited by 208 publications

(169 citation statements)

References 16 publications

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“…Rats were anesthetized with isoflurane (2%-5%) and received full-body blast overpressure (120 ± 7 kPa) exposure, without any shielding to the blast wave, through a pneumatically driven shock tube [1,4,29]. Preoperative buprenorphine (0.05 mg/kg) was then administered through intraperitoneal injection and a reproducible comminuted fracture of the right femur was performed using a drop weight apparatus from a height of 88 cm.…”

Section: Rat Heterotopic Ossification Modelmentioning

confidence: 99%

Early Characterization of Blast-related Heterotopic Ossification in a Rat Model

Qureshi

Crump

Pavey

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Heterotopic ossification (HO) affects the majority of combat-related lower extremity wounds involving severe fracture and amputation. Defining the timing of early osteogenic-related genes may help identify candidate prophylactic agents and guide the timing of prophylactic therapy after blast and other combat-related extremity injuries. Questions/purposes Using a recently developed animal model of combat-related HO, we sought to determine (1) the timing of early chondrogenesis, cartilage formation, and radiographic ectopic bone development; and (2) the early cartilage and bone-related gene and protein patterns in traumatized soft tissue. Methods We used an established rat HO model consisting of blast exposure, controlled femur fracture, crush injury, and transfemoral amputation through the zone of injury. Postoperatively, rats were euthanized on Days 3 to 28. We assessed evidence of early ectopic bone formation by micro-CT and histology and performed proteomic and gene expression analysis. Results All rats showed radiographic evidence of HO within 28 days. Key chondrogenic (collagen type I alpha 1 [COL1a1], p = 0.016) and osteogenic-related genes (RuntThis work was supported by CDMRP (W81XWH-14-2-0010; PI-JAF) and BUMED (602115HP.3720.001.A1014; PI-JAF). Some of the authors are employees of the US Government. This work was prepared as part of their official duties. Title 17 U.S.C. §105 provides that ''Copyright protection under this title is not available for any work of the United States Government.'' Title 17 U.S.C. §101 defined a US Government work as a work prepared by a military service member or employees of the US Government as part of that person's official duties. The opinions or assertions contained in this paper are the private views of the authors and are not to be construed as reflecting the views, policy or positions of the Department of the Navy, Department of Defense nor the US Government. All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research 1 editors and board members are on file with the publication and can be viewed on request. Clinical Orthopaedics and Related Research 1 neither advocates nor endorses the use of any treatment, drug, or device. Readers are encouraged to always seek additional information, including FDA-approval status, of any drug or device prior to clinical use. Each author certifies that his or her institution approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research. This work performed at the Naval Medical Research Center, Silver Spring, MD, USA. Conclusions We found that genes that regulate early chondrogenic and osteogenic signaling and bone development (COL1a1, RUNX-2, OCN, PHEX, and POU5F1) are induced early during the tissue reparative/healing phase in a rat model simulating a combat-related extremity injury. Clinical Relevance The ability to correlate molecular events with histologic and morphologic changes will assist re...

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Section: Rat Heterotopic Ossification Modelmentioning

confidence: 99%

Early Characterization of Blast-related Heterotopic Ossification in a Rat Model

Qureshi

Crump

Pavey

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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“…However, it is unfortunately not standard practice. While few researchers have purposely designed experiments to generate scaled-down exposure conditions [14,32,33] against small mammals, others expose animal models to blast parameters relevant to humans [27,[34][35][36][37][38][39][40][41][42][43]. Assuming mass scaling is relevant to bTBI, some of these exposures may effectively result in exposing an animal model to nuclear-sized blasts [29].…”

Section: Scalingmentioning

confidence: 99%

On the prospective contributions of the shock physics community to outstanding issues concerning blast-induced traumatic brain injury

Ouellet

Petel

2017

Shock Waves

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“…The latter, expansion chamber, is bolted to the larger tube with a Mylar membrane placed between the tubes, allowing compressed air in the expansion chamber to rupture the membrane resulting in a shock wave. Intracranial measures in anesthetized rats demonstrated overpressure within the cerebral ventricles in the range of 30-40 kPa [31], and that blast effects upon intracranial pressure persisted for hours after exposure [24]. Long [28] reports that shock tube exposure at relatively higher levels (126-147 kPa) leads to cerebral hemorrhage, necrosis, cortical cell loss, gliosis, and widespread fiber degeneration, which is not reported in many studies with fluid percussion or control cortical impact injury; others also have reported little evidence of obvious focal cortical injury [25,31].…”

Section: B Blast Overpressure Shock Tubesmentioning

confidence: 99%

“…Intracranial measures in anesthetized rats demonstrated overpressure within the cerebral ventricles in the range of 30-40 kPa [31], and that blast effects upon intracranial pressure persisted for hours after exposure [24]. Long [28] reports that shock tube exposure at relatively higher levels (126-147 kPa) leads to cerebral hemorrhage, necrosis, cortical cell loss, gliosis, and widespread fiber degeneration, which is not reported in many studies with fluid percussion or control cortical impact injury; others also have reported little evidence of obvious focal cortical injury [25,31]. Compared to the methods described in A-C, the shock tube permits whole-body exposure, which is relevant to body effects from blast on neurological function, and studies related to the impact of armor or protective clothing are feasible [25,28].…”

Section: B Blast Overpressure Shock Tubesmentioning

confidence: 99%

Animal models for the study of military-related, blast-induced traumatic brain injury

McCabe¹,

Moratz²,

Liu³

et al. 2010

2010 Biomedical Sciences and Engineering Conference

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Abstract-In present war time conditions, traumatic brain injury (TBI) has moved to the forefront as a "signature injury." In terms of prevalence and understanding the biological mechanisms that underlie the injury, blast-induced TBIparticularly in "mild" cases-has proven to be a significant challenge for military medicine. Basic research that employs animal models of TBI is a key element for furthering our understanding of the biological consequences of blast-related TBI and for the development of therapeutic approaches. This paper outlines the currently available prototypes for the study of TBI, particularly in rodent models, and how the most widely used approaches for modeling TBI can contribute to understanding the mechanisms of injury.

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Measurement of blast wave by a miniature fiber optic pressure transducer in the rat brain

Cited by 208 publications

References 16 publications

Early Characterization of Blast-related Heterotopic Ossification in a Rat Model

Early Characterization of Blast-related Heterotopic Ossification in a Rat Model

On the prospective contributions of the shock physics community to outstanding issues concerning blast-induced traumatic brain injury

Animal models for the study of military-related, blast-induced traumatic brain injury

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