Measurement of bovine subclass antibodies (IgG1 and IgG2) using the amplified enzyme-linked immunosorbent assay

Butler, John E.; Cantarero, Lara; McGivern, P.L.

doi:10.1016/0161-5890(80)90163-7

Cited by 12 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Treatment with Z nS04 alone failed to re move all Fc contamination. The specificity of the anti-y-chain rea gent was evaluated by immunodiffusion in the presence of poly ethylene glycol [30] and by the direct a-ELISA [27,28]. Guinea pig anti-y-chain was prepared and tested as described previously [13], IgG and IgA antibodies tc fluorescein and to OA were mea sured in the a-ELISA using F/-RSA and OA respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Guinea pig anti-y-chain was prepared and tested as described previously [13], IgG and IgA antibodies tc fluorescein and to OA were mea sured in the a-ELISA using F/-RSA and OA respectively. Quantita tion of antibodies in absolute units (/tg/ml) was performed using the 'direct' a-ELISA [27,28] in which known amounts of rabbit IgG or IgA are absorbed on plastic tubes to prepare a standard titration curve. The titration curve for IgG used in these studies showed loglog linearity from 0.38 to 7.5 ng.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Carrier Requirement for Development of Acute Experimental Hypersensitivity Pneumonitis in the Rabbit

Butler¹,

Richerson

Swanson

et al. 1983

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Fluorescein isothiocyanate (FITC) conjugated to protein carriers was used to explore carrier dependence in an established rabbit model of acute hypersensitivity pneumonitis (HSP). Rabbits were immunized via toepads with either FITC-ovalbumin (OA) or FITC-human γ-globulin (HGG) in complete Freund’s adjuvant, and were aerosol challenged with homologous or heterologous conjugates 30 days later. Only those rabbits challenged with the homologous carrier developed acute HSP, despite the presence of comparable levels of anti-FITC antibodies in the sera of all groups. These findings indicate a strict carrier dependence in the pathogenesis of HSP in this model and provide further evidence that the mechanism of inflammation depends upon a cellular immune response.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Carrier Requirement for Development of Acute Experimental Hypersensitivity Pneumonitis in the Rabbit

Butler¹,

Richerson

Swanson

et al. 1983

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…OD41 of nonspecific binding was estimated by addition of PBS instead of test serum and corrected OD49' was obtained by subtracting that of nonspecific binding from each OD41. Data are expressed as means±SE of three or four determinations of ELISA units (corrected OD41 times primary serum dilution factor [=200]) as described elsewhere (18). Sera from PHN rats (PHN; n = 4) and PHN rats treated with daily oral MK886 administration (PHN + MK; n = 3) 10 d after the injection of anti-Fx IA antibody as described above were compared with those from normal control rats (Control; n = 4).…”

Section: Enzyme-linked Immunosorbent Assay For Anti-sheep Igg In Phn mentioning

confidence: 99%

“…ELISA for anti-sheep IgG in PHN rat sera was performed as previously described with slight modification (18). In brief, 100 jd of 200 time-diluted rat test sera was incubated in sheep IgG (Organon Teknika-Cappel, Malvern, PA)-coated 96-well microtiter plates (Becton Dickinson Labware, Lincoln Park, NJ) for 1 h at room temperature.…”

Section: Enzyme-linked Immunosorbent Assay For Anti-sheep Igg In Phn mentioning

confidence: 99%

Leukotriene D4 is a mediator of proteinuria and glomerular hemodynamic abnormalities in passive Heymann nephritis.

Katoh

Lianos

Fukunaga³

et al. 1993

J. Clin. Invest.

View full text Add to dashboard Cite

We assessed the role of leukotrienes (LTs) in Munich-Wistar rats with passive Heymann nephritis (PHN), an animal model of human membranous nephropathy. 10 d after injection of anti-FxlA antibody, urinary protein excretion rate (Upr) in PHN was significantly higher than that of control. Micropuncture studies demonstrated reduced single nephron plasma flow and glomerular filtration rates, increased transcapillary hydrualic pressure difference, pre-and postglomerular resistances, and decreased ultrafiltration coefficient in PHN rats. Glomerular LTB4 generation from PHN rats was increased. Administration of the 5-LO activating protein inhibitor MK886 for 10 d markedly blunted proteinuria and normalized glomerular hemodynamic abnormalities in PHN rats. An LTD4 receptor antagonist SK&F 104353 led to an immediate reduction in Upr and to reversal of glomerular hemodynamic impairment. Ia(+) cells/glomerulus were increased in PHN rats. In x-irradiated PHN rats, which developed glomerular macrophage depletion, augmented glomerular LT synthesis was abolished. Thus, in the autologous phase of PHN, LTD4 mediates glomerular hemodynamic abnormalities and a hemodynamic component of the accompanying proteinuria. The synthesis of LTD4 likely occurs directly from macrophages or from macrophage-derived LTA4, through LTC4 synthase in glomerular cells. (J. Clin. Invest. 1993. 91:1507-1515

show abstract

Application of the peroxidase anti-peroxidase system as an universal reagent for the two-site binding enzyme immunoassay

Porstmann

Seifert

1983

Clinica Chimica Acta

View full text Add to dashboard Cite

Measurement of bovine subclass antibodies (IgG1 and IgG2) using the amplified enzyme-linked immunosorbent assay

Cited by 12 publications

References 11 publications

Carrier Requirement for Development of Acute Experimental Hypersensitivity Pneumonitis in the Rabbit

Carrier Requirement for Development of Acute Experimental Hypersensitivity Pneumonitis in the Rabbit

Leukotriene D4 is a mediator of proteinuria and glomerular hemodynamic abnormalities in passive Heymann nephritis.

Application of the peroxidase anti-peroxidase system as an universal reagent for the two-site binding enzyme immunoassay

Contact Info

Product

Resources

About