MEASUREMENT OF BRADYKININ, PROTEIN, Na+, Cl-, K+, AND Zn++ CONCENTRATIONS IN RAT URINE AFTER INTRAPERITONEAL INJECTION OF Tityus serrulatus VENOM

The aim of this work was to evaluate the hematological changes induced by Tityus serrulatus venom (TsV). Blood of Wistar rats was collected 0.5, 2, 6 and 24 h after i.p. injection of TsV (0.5 mg/kg) or saline (controls). Two additional groups were injected with 0.67 mg/kg and 0.25 mg/kg of TsV and the blood was collected after 0.5 and 2 h, respectively. The results showed an increase on hematocrit (Ht), red blood cells (RBC) count, hemoglobin concentration (Hb), albumin and total protein, mainly 2-6 h after envenoming. Increase in serum activities of amylase, creatine kinase and aspartate aminotransferase were also observed, indicating tecidual damages. Hyperglycemia was observed at all times analyzed, as a consequence of catecholamine release. No significant changes were detected in the urea, [Na(+)] and [Ca(2+)], but an increase of [Mg(2+)], [K(+)] and conductivity was observed. TsV induced a reduction of erythrocytes osmotic fragility as consequence of dehydration and increase in plasma electrolytes concentration, as evidenced by its higher conductivity. This study demonstrated that TsV is able to induce severe hematological changes, that appear within the first hours after envenoming, justifying the seeking of medical attention as soon as possible to avoid worsening of clinical symptoms.

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Isolation and Properties of a New Kallikrein Inhibitor from Tityus serrulatus Venom

Ferreira

Zingalli

Habermehl

et al. 1998

J Protein Chem

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The kallikrein inhibitor-peptide content of Tityus serrulatus scorpion crude venom was purified by Sephadex G-50 and Sephadex G-25 fine gel filtration chromatographies, followed by two steps of reverse-phase column on HPLC. The isolated inhibitor peptide was homogeneous in its N-terminal and partial amino acid sequence, showing a molecular weight of 4.489 Da by mass spectrometry and amino acid analysis. The peptide was tested with rat plasma and urine kallikrein, which resulting in an inhibition with similar affinity to both enzymes, showing an IC50 of 14.3 microM after 13 and 8 min, respectively, using kininogen as substrate on the isolated guinea-pig ileum bioassay. The porcine pancreatic kallikrein showed after 10 min an IC50 value of 12.6 microM with H-D-Val-Leu-Arg-pNA HCl as substrate. In addition, the isolated peptide significantly inhibited porcine pancreatic kallikrein with values in the range of apparent or absolute calculated peptide Ki = 2.5 microM. The inhibitor was heat resistant and stable at pH values less than 5.

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MEASUREMENT OF BRADYKININ, PROTEIN, Na+, Cl-, K+, AND Zn++ CONCENTRATIONS IN RAT URINE AFTER INTRAPERITONEAL INJECTION OF Tityus serrulatus VENOM

Cited by 4 publications

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The kinin system in the envenomation caused by the Tityus serrulatus scorpion sting

The kinin system in the envenomation caused by the Tityus serrulatus scorpion sting

Assessment of biochemical and hematological parameters in rats injected with Tityus serrulatus scorpion venom

Isolation and Properties of a New Kallikrein Inhibitor from Tityus serrulatus Venom

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