Measurement of Calcitonin and Calcitonin Gene–Related Peptide mRNA Refines the Management of Patients with Medullary Thyroid Cancer and May Replace Calcitonin-Stimulation Tests

Camacho, Cleber; Lindsey, Susan C.; Melo, Maria Clara C.; Yang, Ji Hyun; Germano-Neto, Fausto; Valente, Flávia de O.F.; Lima, Thiago Rosa; Biscolla, Rosa Paula M.; Vieira, José Gilberto H.; Cerutti, Janete M.; Silva, Magnus R. Dias da; Maciel, Rui M. B.

doi:10.1089/thy.2012.0361

Cited by 22 publications

(13 citation statements)

References 51 publications

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“…However the sensitivity to detect C‐cell disease remains lower than that of the pentagastrin stimulation test . Provocative testing is sometimes used in follow‐up, for example where basal calcitonin remains within the reference interval but progression is suspected . Samples are usually collected 5 min prior to administration of calcium/pentagastrin and then at intervals of 2, 5 and 7–10 min after.…”

Section: Appendixmentioning

confidence: 99%

Guidelines for the management of thyroid cancer

Perros

Boelaert

Colley

et al. 2014

Clinical Endocrinology

1,103

1,165

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Section: Appendixmentioning

confidence: 99%

Guidelines for the management of thyroid cancer

Perros

Boelaert

Colley

et al. 2014

Clinical Endocrinology

1,103

1,165

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“…Calcitonin (CT), a polypeptide hormone consisting of 32 amino acids, is the major tumor marker for medullary thyroid carcinoma (MTC). CT is mainly produced and rapidly secreted into the blood not only by the thyroidal calcium-sensitive parafollicular C cells but also by the C cell-derived MTC [1][2][3]. MTC itself may either occur sporadically (75 % of the cases) or as part of a familial syndrome named multiple endocrine neoplasia (MEN 2) due to an activating mutation in the RET oncogene [4,5].…”

Section: Introductionmentioning

confidence: 99%

Measurement of Basal Serum Calcitonin for the Diagnosis of Medullary Thyroid Cancer

et al. 2017

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Calcitonin (CT), a tumor marker for medullary thyroid cancer (MTC), can be stimulated with pentagastrin or calcium. Because of the unavailability of pentagastrin, basal CT measurement is frequently used for the preoperative diagnosis of MTC. The aim of the study was to define basal serum calcitonin (bCT) cut-off thresholds for diagnosing MTC. Within a retrospective analysis, 114 patients (51 males) were included fulfilling the criteria of an increased preoperative bCT level (>10 pg/ml) and the criteria of an available postoperative histology analysis. Based on a ROC plot analysis, the cut-off values for the diagnosis of MTC vs. non-malignancy (C cell hyperplasia and goiter) were identified. The most precise bCT thresholds for the identification of MTC were ≥46 pg/ml for males (sensitivity: 93.6%, specificity: 95.0%, PPV: 97%, NPV: 90%) and ≥35 pg/ml for females (sensitivity: 87.3%, specificity: 87.5%, PPV: 98%, NPV: 50%). Using these cut-offs, only 6% of male patients were not identified of having MTC, whereas 5% were false positive (having instead C cell hyperplasia). In females, the discrepancy was higher since 13% of female MTC patients were false negative by using the cut-off of ≥35 pg/ml, and 13% had false positive results (suffering from C cell hyperplasia). Gender-specific bCT cut-offs for the identification of MTC vs. C cell hyperplasia and non-malignancy were defined, which can be used in clinical routine. In female patients, however, the accuracy is much lower compared to males.

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“…To date, differential expression of eS8 gene and its protein has been found in colorectal tumors/polyps [ 13 ] and colorectal carcinoma [ 35 ], respectively. However, its expression is constitutive and ubiquitous among normal and neoplastic thyroid tissues and cell lines [ 36 – 38 ]. This inconsistency of eS8 's expression characteristics across different types of cancer suggests its distinctive association with the type of malignant tissue/cell.…”

Section: Discussionmentioning

confidence: 99%

TheuS8,uS4,eS31, anduL14Ribosomal Protein Genes Are Dysregulated in Nasopharyngeal Carcinoma Cell Lines

Sim

Lee

et al. 2017

BioMed Research International

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The association of ribosomal proteins with carcinogenesis of nasopharyngeal carcinoma (NPC) has been established in a limited subset of ribosomal protein genes. To date, three ribosomal protein genes, eL27 (L27), eL41 (L41), and eL43 (L37a), have been found to be differentially expressed in cell lines derived from NPC tumors. This raises the possibility of more ribosomal protein genes that could be associated with NPC. In this study, we investigated the expression profiles of eight ribosomal protein genes, uS8 (S8), uS4 (S9), eS31 (S27a), eL6 (L6), eL18 (L18), uL14 (L23), eL24 (L24), and eL30 (L30), in six NPC-derived cell lines (HONE-1, SUNE1, HK1, TW01, TW04, and C666-1). Their expression levels were compared with that of a nonmalignant nasopharyngeal epithelial cell line (NP69) using quantitative real-time PCR (RT-qPCR) assay. Of the eight genes studied, the expressions of four ribosomal protein genes uS8 (S8), uS4 (S9), eS31 (S27a), and uL14 (L23) were found to be significantly downregulated in NPC cell lines relative to NP69. Our findings provide novel empirical evidence of these four ribosomal protein genes as NPC-associated genetic factors and reinforce the relevance of ribosomal proteins in the carcinogenesis of nasopharyngeal cancer.

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Measurement of Calcitonin and Calcitonin Gene–Related Peptide mRNA Refines the Management of Patients with Medullary Thyroid Cancer and May Replace Calcitonin-Stimulation Tests

Cited by 22 publications

References 51 publications

Guidelines for the management of thyroid cancer

Guidelines for the management of thyroid cancer

Measurement of Basal Serum Calcitonin for the Diagnosis of Medullary Thyroid Cancer

TheuS8,uS4,eS31, anduL14Ribosomal Protein Genes Are Dysregulated in Nasopharyngeal Carcinoma Cell Lines

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