Measurement of catecholamines and their metabolites

Peaston, R; Weinkove, C.

doi:10.1258/000456304322664663

Cited by 248 publications

(169 citation statements)

References 177 publications

(214 reference statements)

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“…Analysis of catecholamines and metanephrines in plasma and urine can be done by several analytical techniques. Earlier procedures using radioenzymatic assays have generally been superseded by highly sensitive and specific HPLC methods using electrochemical detection (HPLC-ECD) (25,26). Technical improvements have led to the development of a new technique: the liquid chromatography tandem quadrupole mass spectrometry (LC-MS/MS) (27).…”

Section: Which Tests Are Available For the Biochemical Diagnosis Of Pmentioning

confidence: 99%

DIAGNOSIS OF ENDOCRINE DISEASE: Biochemical diagnosis of phaeochromocytoma and paraganglioma

Berkel

Lenders

Timmers

2014

European Journal of Endocrinology

144

112

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Adrenal phaechromocytomas and extra-adrenal sympathetic paragangliomas (PPGLs) are rare neuroendocrine tumours, characterised by production of the catecholamines: noradrenaline, adrenaline and dopamine. Tumoural secretion of catecholamines determines their clinical presentation which is highly variable among patients. Up to 10-15% of patients present entirely asymptomatic and in 5% of all adrenal incidentalomas a PPGL is found. Therefore, prompt diagnosis of PPGL remains a challenge for every clinician. Early consideration of the presence of a PPGL is of utmost importance, because missing the diagnosis can be devastating due to potential lethal cardiovascular complications of disease. First step in diagnosis is proper biochemical analysis to confirm or refute the presence of excess production of catecholamines or their metabolites. Biochemical testing is not only indicated in symptomatic patients but also in asymptomatic patients with adrenal incidentalomas or identified genetic predispositions. Measurements of metanephrines in plasma or urine offer the best diagnostic performance and are the tests of first choice. Paying attention to sampling conditions, patient preparation and use of interfering medications is important, as these factors can largely influence test results. When initial test results are inconclusive, additional tests can be performed, such as the clonidine suppression test. Test results can also be used for estimation of tumour size or prediction of tumour location and underlying genotype. Furthermore, tumoural production of 3-methoxytyramine is associated with presence of an underlying SDHB mutation and may be a biomarker of malignancy.

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Section: Which Tests Are Available For the Biochemical Diagnosis Of Pmentioning

confidence: 99%

DIAGNOSIS OF ENDOCRINE DISEASE: Biochemical diagnosis of phaeochromocytoma and paraganglioma

Berkel

Lenders

Timmers

2014

European Journal of Endocrinology

144

112

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“…To address whether changes in biogenic amines and metabolites caused by various physiological conditions can be quantified by this method, we have examined the Drosophila mutant iav 1 . The iav 1 mutant was initially isolated due to its reduced locomotor activity and its genetic lesion has recently been mapped to the gene coding for the TRPV channel subunit IAV.…”

Section: Mekc-ec Comparison Of Canton-s Wild-type and Inactive Drosopmentioning

confidence: 99%

Microcolumn Separation of Amine Metabolites in the Fruit Fly

et al. 2005

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Electrophoretic resolution of fourteen biogenic amines and metabolites with similar mobilities is addressed by employing micellar electrokinetic capillary chromatography coupled to amperometric electrochemical detection. The present study describes the optimization of separation conditions to achieve resolution of analytes of biological significance within 20 minutes in a single separation. They include dopamine, epinephrine, norepinephrine, octopamine (OA), L-3, 4-dihydroxyphenylalanine, tyramine (TA), and serotonin as well as metabolites 5-hydroxyindolacetic acid, 3,4-dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine in addition to Nacetylated metabolites including N-acetyl dopamine, N-acetyl octopamine (naOA), and N-acetyl serotonin. The optimized conditions used result in excellent reproducibility and predictable peak shifting, thus enabling identification of several metabolites along with their biogenic amine precursors in biological samples, specifically from the fruit fly Drosophila melanogaster. The separation method is sensitive, selective and quantitative as demonstrated by its capacity to detect changes in TA, OA, and naOA present in the head homogenates of the Canton-S and mutant inactive 1 Drosophila lines. Quantitative analysis of metabolites in conjunction with their biogenic amine precursors in a single separation offers tremendous potential to understand the physiological processes and underlying mechanisms mediated by various biogenic amines in Drosophila and other animals.

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“…1 Prior to chromatography, sample clean-up is commonly performed using one or two solid-phase extraction (SPE) columns to remove potentially interfering substances. With the increasing availability of bench-top tandem mass spectrometers in the clinical laboratory, it is possible that these detectors could substitute for ECD as highly specific and robust analytical instruments for urinary catecholamines 3 and metanephrines.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous measurement of urinary metanephrines and catecholamines by liquid chromatography with tandem mass spectrometric detection

Whiting

2009

Ann Clin Biochem

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Background: The measurement of catecholamines and metanephrines in urine is an important diagnostic test in biochemical screening for phaeochromocytoma. Tandem mass spectrometry (MSMS) has the potential to be used in a profiling method for simultaneous assay of these analytes. Methods: Optimal conditions were established for the MSMS detection of catecholamines (noradrenalin, adrenalin and dopamine) and metanephrines (normetanephrine and metanephrine), including commercially available isotopically labelled compounds for use as internal standards. Chromatographic separation of all five polar biogenic amines was achieved under solvent conditions that were compatible with MSMS and multiple reaction monitoring. Several types of solid-phase extraction cartridge were used to investigate clean-up conditions for urine, and acid-hydrolysates of urine, prior to LC-MSMS. Results: Total catecholamines and metanephrines from acid-hydrolysed urines, or free catecholamines and free metanephrines from native urines, were complexed with diphenyl-boronate and recovered in high yield from polymer cartridges after elution with formic acid. Direct injection of eluates into the LC-MSMS system allowed quantitation of catecholamines and metanephrines with a run time of 6 min per sample. Biogenic amine concentrations for patient urines and quality assurance programme samples, and assay imprecision, were similar to values obtained with high-performance liquid chromatography methods, which used electrochemical detection. In normal urines, the ratio of free to total catecholamines was around three-fold higher than the ratio of free to total metanephrines. Conclusion: The assay of urinary catecholamines and metanephrines can be achieved simultaneously using one LC-MSMS method, which is rapid and reduces labour and consumable costs for routine application.

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Measurement of catecholamines and their metabolites

Cited by 248 publications

References 177 publications

DIAGNOSIS OF ENDOCRINE DISEASE: Biochemical diagnosis of phaeochromocytoma and paraganglioma

DIAGNOSIS OF ENDOCRINE DISEASE: Biochemical diagnosis of phaeochromocytoma and paraganglioma

Microcolumn Separation of Amine Metabolites in the Fruit Fly

Simultaneous measurement of urinary metanephrines and catecholamines by liquid chromatography with tandem mass spectrometric detection

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