Measurement of Cell Lysis by Light Scattering

Valenzeno, Dennis Paul; Trank, J W

doi:10.1111/j.1751-1097.1985.tb08950.x

Cited by 55 publications

(24 citation statements)

References 14 publications

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“…Valenzeno and Trank proposed a technique for measurement of the blood haematocrit based on the decrease of forward scattering during erythrocyte lysis. The loss of refractive index mismatches reduced the scattering coefficient and anisotropy [38]. The degree of haemolysis is inversely related to the intensity of small angle scattering.…”

Section: Discussionmentioning

confidence: 99%

Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography

et al. 2009

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Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm−1 to 1.3 ± 0.6 mm−1, whereas, in the apoptotic cells, an increase to 6.4 ± 1.7 mm−1 was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography

et al. 2009

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“…The fractional H was scaled to zero at OD 750 immediately after irradiation and scaled to unity after OD 750 had attained a constant value. The linearity of the light scattering assay was shown in previous work [1,3,20]. This technique was re-evaluated by hemolyzing RBC suspensions in distilled water and measuring the hemoglobin absorption after centrifugation.…”

Section: Methodsmentioning

confidence: 99%

A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX

Al-Akhras

2006

Med Bio Eng Comput

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Photosensitization by protoporphyrin IX (PpIX) is accelerated at different irradiation temperatures, different dark incubation temperatures (Tinc) and different irradiation times. The applicability of Gompertz function to the fractional photohemolysis ratio, a and the rate of fractional photohemolysis, b is found to be the most appropriate model to fit the experimental data with minimum parameters and minimum errors. The reduction in Gompertz parameters, the fractional ratio values of a, and increase in the fractional rate values b, for 20 microM PpIX irradiated with black light at low irradiation temperature 5 degrees C and higher Tinc 37 degrees C was noticed. The parameter a has higher values at lower irradiation time and lower irradiation temperatures which indicates a longer photohemolysis process and longer t 50. Values of the parameter b were found to be strongly temperature-dependent, and always increase with increasing irradiation time and Tinc with lower values at lower irradiation time and lower Tinc. There are no significant changes in the lysis of RBCs process at irradiation temperatures equal to or higher than 35 degrees C. Similarly, no significant change on t50 at higher irradiation time at Tinc 24 and 37 degrees C. In conclusion, Gompertz analysis technique adapts to study the photohemolysis process at different conditions as a best-fit model.

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“…Cell membrane photodamage was investigated by using albino mouse erythrocytes as model system [16]. The hemolysis was followed by measuring the decrease in absorbance at 650 nm, which is proportional to the number of intact cells [17], and the results are reported in Fig. 6.…”

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confidence: 99%

Photophysical and Phototoxic Properties of the Antibacterial Fluoroquinolones Levofloxacin and Moxifloxacin

Viola

Facciolo

Canton

et al. 2004

Chemistry & Biodiversity

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Two antibacterial fluoroquinolones, levofloxacin and moxifloxacin, were investigated to evaluate their photophysical properties and to explore the mechanism of their phototoxicity. Photophysical experiments were carried out in aqueous solution by stationary and time-resolved fluorimetry, and by laser flash photolysis, to obtain information on the various decay pathways of the excited states of the drugs and on transient species formed upon irradiation. The results obtained show that levofloxacin is able to photosensitize red blood cell lysis in an oxygen-independent way and induce a high decrease in cell viability after UVA irradiation, although to a lesser degree than the racemic mixture ofloxacin. Moxifloxacin, which is an 8-MeO-substituted fluoroquinolone, is less phototoxic than the other compounds. Cellular phototoxicity was inhibited by the addition of superoxide dismutase, catalase, and free radical and hydroxyl radical scavengers (BHA, GSH, mannitol, and DMTU), indicating the involvement of superoxide anion and/or a radical mechanism in their cytotoxicity. A good correlation was observed between lipid peroxidation, protein photodamage, and cellular phototoxicity, indicating that test compounds exert their toxic effects mainly in the cellular membrane. Experiments carried out on pBR322 DNA show that these derivatives do not significantly photocleave DNA directly, but single-strand breaks were evidenced after treatment of photosensitized DNA by two base-excision-repair enzymes, and Endo III.

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Measurement of Cell Lysis by Light Scattering

Cited by 55 publications

References 14 publications

Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography

Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography

A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX

Photophysical and Phototoxic Properties of the Antibacterial Fluoroquinolones Levofloxacin and Moxifloxacin

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