Measurement of cell migration stimulated by interleukin 8: Use of ATP chemiluminescence

McCafferty, Ann C.; Cree, Ian A.

doi:10.1016/1043-4666(94)90070-1

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…As the amount of intracellular ATP could be correlated to actin-myosin interactions and thus to cellular retraction at the rear of the cell, an ATP assay was performed to support our data. Indeed, such a method has already been used in a number of studies to assess migratory activities ( Figure 6C) (Partsch and Schwarzer, 1991;McCafferty and Cree, 1994). The data revealed an approximately 2-fold Myogenic cells were transfected using 50nM antisense oligomers directed against each calpain (see legend Figure 1).…”

Section: Migration Was Prevented By Marcks and Psd-mutated Marcks Ovementioning

confidence: 99%

Myoblast migration is prevented by a calpain‐dependent accumulation of MARCKS

Dedieu

Mazères

Poussard

et al. 2003

Biology of the Cell

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Calpains, also called calcium activated neutral cysteine proteases are presently known to play pivotal roles in physiological and biological phenomena such as signal transduction, cell spreading and motility, apoptosis, regulation of cell cycle and regulation of muscle cell differentiation. Concerning this last point, calpains have been shown to play a crucial role during the earlier myogenesis. In this study we have analyzed the involvement of calpains during an important step of myogenesis: myoblast migration. Our findings show that myoblast migration was drastically reduced when the expression of µ-and m-calpain was decreased. We have also observed that MARCKS (myristoylated alanine rich C kinase substrate), a protein localized at focal adhesion sites, was significantly accumulated when the expression levels of calpains were decreased. Also, using phorbol myristate acetate, (an activator of PKC) and plasmids carrying the full-length cDNA of MARCKS or a cDNA fragment lacking the phosphorylation site domain, we demonstrated that normal myoblast migration is dependent on MARCKS phosphorylation and localization.

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Section: Migration Was Prevented By Marcks and Psd-mutated Marcks Ovementioning

confidence: 99%

Myoblast migration is prevented by a calpain‐dependent accumulation of MARCKS

Dedieu

Mazères

Poussard

et al. 2003

Biology of the Cell

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“…Toxicity was measured by determining ATP content with the ViaLight HS kit (Lumitech Ltd., Nottingham, U.K.) (28). The protein solutions at 100 µM concentrations were preincubated at 37 °C for 3 days and 10-fold diluted with the culture medium before the solutions were added to the cells.…”

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confidence: 99%

Dimeric Transthyretin Variant Assembles into Spherical Neurotoxins

et al. 2005

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Familial amyloidotic polyneuropathy is a hereditary autosomal-dominant disease in which the deposited transthyretin fibrils are derived from amyloidogenic mutation. We investigated structure and stability of a human Ser112Ile transthyretin variant and showed that the Ser112Ile variant exists as a dimer having nonnative tertiary structure at physiological pH. In addition, the dimeric Ser112Ile assembles into a spherical aggregate and exerts cytotoxicity in a human neuroblastoma cell line. Our results suggest the importance of an unstable dimeric structure in forming spherical aggregates that will induce cell death.

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The effect of cefodizime on phagocyte function in non-patient volunteers and patients with chronic renal failure

McCafferty¹,

McGregor

Jones

et al. 1996

Int J Clin Lab Res

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Cefodizime has previously been shown to possess a number of immunomodulating properties in vivo and in vitro using several different test systems. Since most in vitro studies have been performed with cells from normal individuals, we first investigated whether cells from chronic renal failure patients would respond in vitro to cefodizime in the same way as healthy subjects. Subsequently, we investigated the effect of cefodizime (10 g over 10 days in 2-g doses) on phagocyte function ex vivo in an open study of 26 chronic renal failure patients and 16 healthy subjects. Polymorphonuclear leukocytes were tested for their ability to polarize in response to cefodizime and/or f-met-leu-phe peptide. Polymorphonuclear leukocytes and monocytes were tested for their ability to produce chemiluminescence on stimulation with either phagocytic (zymosan) or soluble phorbol myristate acetate stimuli. Phagocyte and lymphocyte membrane receptor expression was compared after exposure to cefodizime. Exposure to cefodizime in vitro causes a significant increase in polarization of polymorphonuclear leukocytes from both normal individuals and renal failure patients (both P < 0.001). It also caused increased chemotaxis and chemokinesis in a modified Boyden chamber assay. Cefodizime did not affect lucigenin-enhanced chemiluminescence and there were only minor effects on cell membrane antigen levels. In the ex vivo study there was a significant increase in polymorphonuclear leukocyte polarization (P < 0.001) attributable to cefodizime, but other investigations showed no significant differences. The results suggest that cefodizime may act as a mild priming agent for some functions, particularly chemotaxis.

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Measurement of cell migration stimulated by interleukin 8: Use of ATP chemiluminescence

Cited by 4 publications

References 12 publications

Myoblast migration is prevented by a calpain‐dependent accumulation of MARCKS

Myoblast migration is prevented by a calpain‐dependent accumulation of MARCKS

Dimeric Transthyretin Variant Assembles into Spherical Neurotoxins

The effect of cefodizime on phagocyte function in non-patient volunteers and patients with chronic renal failure

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