Measurement of Cell Wall Volume using Confocal Microscopy and its Application to Studies of Forage Degradation

Travis, Anthony J.; Murison, Sandra D; Perry, Paul; Chesson, Andrew

doi:10.1006/anbo.1997.0420

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…The use of these systems is advantageous for the analysis of large numbers of samples in routine applications and allows us to overcome problems due to subjectivity (van der Heijden et al 1995;Travis et al 1996Travis et al , 1997. Inconsistencies between descriptive and quantitative results of anatomical studies might be explained by the differences in the methodological approaches.…”

Section: Discussionmentioning

confidence: 96%

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Method to classify xylem cell types using cross sectionsof 1-year-old branches in Mediterranean woody species

Micco

Toraldo

Aronne

2006

Trees

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Measurements of anatomical parameters of wood are of great interest both for eco-physiological purposes and for technological applications. The aim of this paper is to describe a new method for classifying and measuring cell lumen of xylem, analysing cross sections under the light microscope. The proposed method is based on the application of digital image analysis on images of the cross sections of xylem in combination with graphical and statistical methods. The methodology was tested on 1-year-old branches of several woody species, both trees and shrubs, occurring in a Mediterranean natural ecosystem in southern Italy. The development of the procedure was based on statistical comparison between data collected according to four procedures: (a) manual identification and measurement of lumen diameter of conduits on longitudinal sections; (b) manual identification and measurement of lumen diameter of conduits on cross sections; (c) manual identification and measurement of lumen area of conduits on cross sections; and (d) automatic measurement of lumen area of conduits on cross sections. The influence of image resolution and that of the position of the selected area on cell classification and measurements were ascertained. The proposed method was proved to be specific to woody structures and allowed the construction of a model-graph that is species-specific. Interpretation of the model-graphs allows classification and hence measurement of identified cells. Communicated by M. Buckeridge V. De Micco ( ) · G. Aronne

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Section: Discussionmentioning

confidence: 96%

“…Suitable methods for routine applications have been proposed for the detection of cell wall thickness and lignification to determine forage degradation, or for the measurement of cell wall dimensions to predict the resistance to bending of cut flowers (van der Heijden et al 1995;Travis et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Method to classify xylem cell types using cross sectionsof 1-year-old branches in Mediterranean woody species

Micco

Toraldo

Aronne

2006

Trees

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“…(Beauchemin et al, 1995;. Forage heterogeneity contributes largely to the variation in degradability of plant material even with similar chemical composition but varied anatomy (Travis et al, 1997). It is thus important to recognize the botanical characteristics of plant material as a factor determining its nutritional value (Walters, 1971).…”

Section: Introductionmentioning

confidence: 99%

Exogenous fibrolytic enzymes to unlock nutrients: Histological investigation of its effects on fibre degradation in ruminants

Vyver¹,

Cruywagen²

2014

SA J. An. Sci.

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There is a need for a better understanding of the mode-of-action of exogenous fibrolytic enzymes (EFE) used as additives in ruminant feeds. Four forages, treated with EFE, were evaluated in vitro and histologically, in an attempt to determine the effect of EFE on tissue degradation. Weeping love grass, kikuyu leaf material, lucerne and wheat straw stem material were histologically evaluated. Simultaneously, milled samples were incubated in the rumen fluid inoculated media for the determination of in vitro digestibility. The main focus, however, was a quantitative assessment of the degradation of the plant tissue at histological level over a 24 h incubation period. Degradation of cell wall components were quantified using the image analysis software. After 12 h of incubation, cell walls of the metaxylem of kikuyu and weeping love grass leaf material were thinner for the EFE treatment than for the control treatment. Treatment also resulted in a significant thinning effect of the cell walls of kikuyu phloem (12 h) and the adaxial epidermis (24 h). The abaxial epidermis at 12 h was thinner for weeping love grass due to EFE treatment. For stem material, a thinning of the epidermis of EFE treated lucerne was observed. Histological findings were concomitant with higher in vitro digestibilities of EFE treated lucerne and kikuyu. It was concluded that image analysis can be useful to quantify changes in cell wall due to the treatment of forages with EFE. There was a definite, subtle thinning effect of EFE on cell wall thickness of plant material which could be indicative of the mode-of-action of EFE.________________________________________________________________________________________________________________________

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“…As diferenças anatômicas das plantas, os sítios de lignificação e os fatores que afetam a degradação dos tecidos vegetais pelos microrganismos do rúmen podem ser analisados pelas técnicas de microscopia e, assim, auxiliar na avaliação do valor nutricional de plantas forrageiras (Akin et al, 1974;van der Heijden et al, 1995;Engels, 1996;Travis et al,1997).…”

Section: Introductionunclassified

Características anatômicas e valor nutritivo de quatro gramíneas predominantes em pastagem natural de Viçosa, MG

Bauer¹,

Gomide²,

Silva³

et al. 2008

R. Bras. Zootec.

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RESUMO -Avaliou-se a influência das características anatômicas e dos sítios de lignificação dos tecidos sobre o valor nutritivo de lâminas foliares de quatro gramíneas coletadas nas estações chuvosa e seca. Amostras frescas das duas últimas lâminas foliares recém-expandidas, correspondentes à última e à penúltima posição no perfilho de capim-gordura (Melinis minutiflora Pal. De Beauv), capim-braquiária (Brachiaria decumbens Staph.), capim-sapé (Imperata brasiliensis Trin.) e capim-jaraguá (Hyparrhenia rufa (Nees) Staph.) foram avaliadas quanto às características anatômicas, segundo técnicas de microscopia de luz e varredura. Nessas amostras analisou-se a proporção de tecidos e de sítios de lignificação. Também foram determinadas as concentrações de fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), lignina e celulose da parede celular, assim como a digestibilidade in vitro da matéria seca (DIVMS). Os dados foram analisados segundo delineamento inteiramente casualizado, com os tratamentos no esquema fatorial, com três repetições. Determinaram-se as correlações entre DIVMS, componentes químicos da parede celular e proporção de tecidos. Observou-se o mesmo padrão de proporção de tecidos e DIVMS no capim-gordura e no capim-braquiária, assim como nos capins sapé e jaraguá. A digestibilidade in vitro da matéria seca e os componentes da parede celular sofreram influência da estação, enquanto as características anatômicas não foram significativamente influenciadas por esse fator. Os capins gordura e braquiária caracterizaram-se por maiores proporções de bainha parenquimática do feixe e os capins sapé e jaraguá por maiores

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Measurement of Cell Wall Volume using Confocal Microscopy and its Application to Studies of Forage Degradation

Cited by 15 publications

References 24 publications

Method to classify xylem cell types using cross sectionsof 1-year-old branches in Mediterranean woody species

Method to classify xylem cell types using cross sectionsof 1-year-old branches in Mediterranean woody species

Exogenous fibrolytic enzymes to unlock nutrients: Histological investigation of its effects on fibre degradation in ruminants

Características anatômicas e valor nutritivo de quatro gramíneas predominantes em pastagem natural de Viçosa, MG

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