Measurement of CGRP in dried blood spots using a modified sandwich enzyme immunoassay

Song, Eun Young; VanDunk, Cassandra; Kuddo, Thea; Nelson, Phillip G.

doi:10.1016/j.jneumeth.2005.12.020

Cited by 4 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to CCT, CGRP is a member of the CCT family of peptides, is expressed in brain neurons and is elevated in the blood of ASD infants [64]. Elevated CGRP levels have been reported when assayed in newborn dried bloodspots and CGRP is known to be a neurotransmitter, neuromodulator and a trophic nerve factor [65]. Finally, it is germane to this discussion that CGRP is regulated by cytokines such as TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Newborn Screening for Autism: in Search of Candidate Biomarkers

Mizejewski

Lindau-Shepard

Pass³

2013

Biomarkers Med.

View full text Add to dashboard Cite

Background Autism spectrum disorder (ASD) represents a wide range of neurodevelopmental disorders characterized by impairments in social interaction, language, communication and range of interests. Autism is usually diagnosed in children 3–5 years of age using behavioral characteristics; thus, diagnosis shortly after birth would be beneficial for early initiation of treatment. Aim This retrospective study sought to identify newborns at risk for ASD utilizing bloodspot specimens in an immunoassay. Materials & methods The present study utilized stored frozen specimens from ASD children already diagnosed at 15–36 months of age. The newborn specimens and controls were analyzed by immunoassay in a multiplex system that included 90 serum biomarkers and subjected to statisical analysis. Results Three sets of five biomarkers associated with ASD were found that differed from control groups. The 15 candidate biomarkers were then discussed regarding their association with ASD. Conclusion This study determined that a statistically selected panel of 15 biomarkers successfully discriminated presumptive newborns at risk for ASD from those of nonaffected controls.

show abstract

Section: Discussionmentioning

confidence: 99%

Newborn Screening for Autism: in Search of Candidate Biomarkers

Mizejewski

Lindau-Shepard

Pass³

2013

Biomarkers Med.

View full text Add to dashboard Cite

show abstract

Headache in Individuals with Neurodevelopmental Disorders

Chadehumbe¹

2023

Curr Pain Headache Rep

View full text Add to dashboard Cite

Current and future use of “dried blood spot” analyses in clinical chemistry

Lehmann¹,

Delaby

Vialaret

et al. 2013

Clinical Chemistry and Laboratory Medicine (CCLM)

120

View full text Add to dashboard Cite

Abstract:The analysis of blood spotted and dried on a matrix (i.e., "dried blood spot" or DBS) has been used since the 1960s in clinical chemistry; mostly for neonatal screening. Since then, many clinical analytes, including nucleic acids, small molecules and lipids, have been successfully measured using DBS. Although this preanalytical approach represents an interesting alternative to classical venous blood sampling, its routine use is limited. Here, we review the application of DBS technology in clinical chemistry, and evaluate its future role supported by new analytical methods such as mass spectrometry.

show abstract

Measurement of CGRP in dried blood spots using a modified sandwich enzyme immunoassay

Cited by 4 publications

References 17 publications

Newborn Screening for Autism: in Search of Candidate Biomarkers

Newborn Screening for Autism: in Search of Candidate Biomarkers

Headache in Individuals with Neurodevelopmental Disorders

Current and future use of “dried blood spot” analyses in clinical chemistry

Contact Info

Product

Resources

About