Measurement of Cholesterol and Triglycerides in Dried Serum and the Effect of Storage

Reddy, K. Srinath; Jailkhani, B. L.

doi:10.1093/clinchem/47.6.1113

Cited by 18 publications

(6 citation statements)

References 7 publications

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“…Alternatively, dried blood samples on filter paper have the advantages of easy storage and transport, as well as being less intrusive (i.e., by finger prick rather than by venipuncture). Dried blood spots have been recommended by the World Health Organization for use in field HIV-1 investigations and have been used in various studies within India [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Prospective Study of One Million Deaths in India: Rationale, Design, and Validation Results

et al. 2005

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BackgroundOver 75% of the annual estimated 9.5 million deaths in India occur in the home, and the large majority of these do not have a certified cause. India and other developing countries urgently need reliable quantification of the causes of death. They also need better epidemiological evidence about the relevance of physical (such as blood pressure and obesity), behavioral (such as smoking, alcohol, HIV-1 risk taking, and immunization history), and biological (such as blood lipids and gene polymorphisms) measurements to the development of disease in individuals or disease rates in populations. We report here on the rationale, design, and implementation of the world's largest prospective study of the causes and correlates of mortality.Methods and FindingsWe will monitor nearly 14 million people in 2.4 million nationally representative Indian households (6.3 million people in 1.1 million households in the 1998–2003 sample frame and 7.6 million people in 1.3 million households in the 2004–2014 sample frame) for vital status and, if dead, the causes of death through a well-validated verbal autopsy (VA) instrument. About 300,000 deaths from 1998–2003 and some 700,000 deaths from 2004–2014 are expected; of these about 850,000 will be coded by two physicians to provide causes of death by gender, age, socioeconomic status, and geographical region. Pilot studies will evaluate the addition of physical and biological measurements, specifically dried blood spots.Preliminary results from over 35,000 deaths suggest that VA can ascertain the leading causes of death, reduce the misclassification of causes, and derive the probable underlying cause of death when it has not been reported. VA yields broad classification of the underlying causes in about 90% of deaths before age 70. In old age, however, the proportion of classifiable deaths is lower. By tracking underlying demographic denominators, the study permits quantification of absolute mortality rates. Household case-control, proportional mortality, and nested case-control methods permit quantification of risk factors.ConclusionsThis study will reliably document not only the underlying cause of child and adult deaths but also key risk factors (behavioral, physical, environmental, and eventually, genetic). It offers a globally replicable model for reliably estimating cause-specific mortality using VA and strengthens India's flagship mortality monitoring system. Despite the misclassification that is still expected, the new cause-of-death data will be substantially better than that available previously.

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Section: Discussionmentioning

confidence: 99%

Prospective Study of One Million Deaths in India: Rationale, Design, and Validation Results

et al. 2005

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“…Cholesterol and triglyceride measurements from dried blood have been reported previously under controlled laboratory conditions. [7][8][9] The collection of blood samples by finger pricks from subjects living in places with limited resources and transportation to a distant laboratory will be useful for screening with a wider reach. However, the suitability for centrifugation are not accessible.…”

Section: Discussionmentioning

confidence: 99%

“…Methods for the measurement of cholesterol and triglycerides from dried blood have been reported previously by our group. [7][8][9] This article presents results of total cholesterol and triglyceride values obtained from dried blood as compared to values obtained in serum.…”

Section: Introductionmentioning

confidence: 99%

Utility of Dried Blood Spots for Measurement of Cholesterol and Triglycerides in a Surveillance Study

Gupta

Prabhakaran

Snehi

et al. 2010

Journal of Diabetes Science and Technology

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“…Cell lysates and culture supernatants were then obtained and stored at -80 o C prior to testing. Ezymatic colorimetric assays were used to measure cholesterol, triglycerides and glucose (using Diasys colorimetric kits, Diagnostic systems) in comparison with provided known standard (DIASYS) [24,25].…”

Section: Cholesterol Triglyceride and Glucose-intake Measurementsmentioning

confidence: 99%

Potential anti-adipogenic activity ofCalligonum comosumcuminaldehyde on mouse 3T3-preadipocytes

Mohammad

El‐Serafi

Madkour

et al. 2020

Preprint

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AbstractObesity is a medical condition associated with serious medical and psycho-social consequences and an augmented body fat mass. Several compounds were suggested to counteract obesity and fat accumulation with variable degrees of success. Searching for a safe and effective anti-adipogenic substance, we found that cuminaldehyde-rich essential oil extracted from Calligonum comosum potentially mediate activities. The results showed that C. comosum essential oil and its major component cuminaldehyde, selectively caused significant reduction in the viability of 3T3-L1 cells when compared to fibroblasts. Furthermore, cuminaldehyde caused significant reduction in the lipid content, glucose uptake and levels of both triglycerides and cholesterol in adipocytes. Moreover, the formation of 3D-adipocyte pellets in the presence of cuminaldeyde was affected. Adipocytes matured in the presence of cuminaldehyde have significant reduction in the expression of adipocyte-specific transcripts, CAAT-enhancer binding protein alpha (CEBPa) and Peroxisome proliferator-activated receptor gamma (PPARg). Taken together, these results demonstrate a potential inhibitory role of cuminaldehyde extracted from C. comosum oil on lipid accumulation. Consequently, cuminaldeyde can be considered as a new potential anti-adipogenic agent for prevention and treatment of obesity.

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Measurement of Cholesterol and Triglycerides in Dried Serum and the Effect of Storage

Cited by 18 publications

References 7 publications

Prospective Study of One Million Deaths in India: Rationale, Design, and Validation Results

Prospective Study of One Million Deaths in India: Rationale, Design, and Validation Results

Utility of Dried Blood Spots for Measurement of Cholesterol and Triglycerides in a Surveillance Study

Potential anti-adipogenic activity ofCalligonum comosumcuminaldehyde on mouse 3T3-preadipocytes

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