Measurement of Creatine kinase and Aspartate aminotransferase in saliva of dogs: a pilot study

Abstract

BackgroundMuscle enzymes in saliva have been reported to be possible markers of heart and muscle damage in humans. The aim of this study was to assess if Creatine kinase (CK) and Aspartate aminotransferase (AST) activities could be measured in canine saliva, and to evaluate their possible changes in situations of muscle damage.ResultsThe spectrophotometric assays for CK and AST measurement in saliva of dogs showed intra- and inter-assay imprecision lower than 1 and 16% and coefficients of correlation close to 1 in linearity under dilution tests. Healthy dogs showed activities in saliva of CK between 27 and 121 U/L and AST between 46 and 144 U/L, whereas in saliva of dogs with muscle damage CK ranged between 132 and 3862 U/L and AST between 154 and 4340 U/L. Positive moderate correlations were found between saliva and serum activities of the two enzymes (CK, r = 0.579; P = 0.001; AST, r = 0.674; P = 0.001).ConclusionsCK and AST activities can be measured in canine saliva with commercially available spectrophotometric assays. In addition these enzymes show higher values in saliva of dogs with muscle damage and their values are moderately correlated with those of serum.

Preliminary evaluation of a salivary urea test strip method for use in dogs

“…The analysis of salivary biomarkers has gained increasing popularity in recent years particularly since the collection of saliva is easy and noninvasive. Salivary urea and creatinine concentrations (determined on a biochemistry analyzer) are significantly increased in dogs with CKD; and therefore, these parameters can be used as markers of renal failure .…”

Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities

“…8,9 In addition these enzymes in saliva can change in situations of intensive exercise, 10 peptic ulcers, 11 myocardial infarction 12 and muscle damage. 13 To the authors' knowledge, no data about any possible influence of pre-analytical factors on measurements of these three enzymes in saliva have been reported. Thus, the aim of this study was to investigate the impact of saliva collection and processing methods on their measurements.…”

Verlaufsuntersuchungen bei Deutschen Jagdterriern mit belastungsabhängiger metabolischer Myopathie

“…Als Ursache für die Braunfärbung des Urins dieser Hunde wurde eine Myoglobinurie angenommen [14], da eine Hämolyse anhand einer mikroskopischen Beurteilung des Urinsediments und durch fehlende rötliche Verfärbung des Blutplasmas nach Zentrifugation differenzialdiagnostisch ausgeschlossen werden konnte [26]. Da die CK einen sensitiven Marker für das Ausmaß einer Muskelzellzerstörung der quergestreiften Muskulatur darstellt [26] [32] und die restlichen Leberenzyme sowie die hämatologischen Parameter unauffällige Werte aufwiesen, wurde als Ursache der veränderten Laborwerte eine Rhabdomyolyse angenommen [14][26] [32]. Diese These ließ sich durch histologische Untersuchung von Muskelbioptaten mit Nachweis einer Muskelzellnekrose, formveränderter Mitochondrien und einer vermehrten Lipideinlagerung zwischen den Skelettmuskelfasern beweisen [14].…”