Measurement of cystatin C in human urine by particle-enhanced turbidimetric immunoassay on an automated biochemistry analyzer

Makris, Konstantinos; Nikolaki, Efthimia; Nanopoulos, Konstantinos; Pirgakis, Konstantinos M.; Maltezos, Chrisostomos

doi:10.1016/j.clinbiochem.2013.05.072

Cited by 12 publications

(9 citation statements)

References 14 publications

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“…Our assay properties seem to be similar or even better than those of others [2,22,23]. Our LoD is slightly lower than that of the turbidimetric method reported by Makris et al [23].…”

Section: Analytical Performancecontrasting

confidence: 48%

“…Although u-CYSC is reported to be a reliable marker of tubular dysfunction, up to now only a few automated turbidimetric or nephelometric serum CYSC assays were adapted for urinary measurements [2,3,5,22,23]. Our assay properties seem to be similar or even better than those of others [2,22,23]. Our LoD is slightly lower than that of the turbidimetric method reported by Makris et al [23].…”

Section: Analytical Performancecontrasting

confidence: 47%

“…On the other hand, in severe cases of renal tubular damage but practically for all urine samples above 1.0 mg/l of u-CYSC (that is the calibrated range) the test should be repeated after a proper dilution of the urine samples due to the experienced high-dose hook effect. Although u-CYSC is reported to be a reliable marker of tubular dysfunction, up to now only a few automated turbidimetric or nephelometric serum CYSC assays were adapted for urinary measurements [2,3,5,22,23]. Our assay properties seem to be similar or even better than those of others [2,22,23].…”

Section: Analytical Performancementioning

confidence: 86%

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Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

et al. 2018

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Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.

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“…Our assay properties seem to be similar or even better than those of others [2,22,23]. Our LoD is slightly lower than that of the turbidimetric method reported by Makris et al [23].…”

Section: Analytical Performancecontrasting

confidence: 48%

Section: Analytical Performancecontrasting

confidence: 47%

Section: Analytical Performancementioning

confidence: 86%

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Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

et al. 2018

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“…Similarly average value (12.65 ng/mL) for NGAL was calculated from studies by Lacquaniti et al and Wo et al [31,32]. The values of NGAL and Cystatin C in diabetes and pre-diabetes with normoalbuminuria were higher than the values in healthy subjects found by previous studies [28][29][30][31][32]. The values of Cystatin C in diabetes subjects with normoalbuminuria and microalbuminuria were 0.06 ± 0.06 and 0.32 ± 1.14 mg/L, respectively according to Jeon et al [9].…”

Section: Discussionmentioning

confidence: 94%

Novel urinary biomarkers in pre-diabetic nephropathy

et al. 2015

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“…They were comparable to those previously published. 5,10 The hook effect, which can lead to falsely low results in conditions of antigen excess, represents a potential problem in homogeneous immunoassay methods for analytes with a wide range of concentrations as well described for urinary albumin. 13,14 It is also the case for urinary Cyst C values that ranges from undetectable values in healthy volunteers to more than 30 mg/L in patients with tubular injury.…”

Section: Resultsmentioning

confidence: 99%

Analytical performances of PENIA and PETIA urinary cystatin C determination allow tubular injury investigation

et al. 2018

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Background The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction. Methods CysC in urine was measured by a particle-enhanced nephelometric immunoassay using Siemens reagents and by a particle-enhanced turbidimetric immunoassay using DiaSys reagents. Imprecision, linearity, limit of detection and limit of quantification were evaluated according to CLSI recommendations. The two methods were tested on 150 urinary samples from 50 healthy subjects, 50 HIV patients with tubular dysfunction and 50 patients who developed acute kidney acute injury. Results Within-laboratory coefficients of variations were below 4%. The lower limit of quantification of the assay was found at 0.043 and 0.046 mg/L for DiaSys and Siemens, respectively. The following Passing-Bablok regression equations were obtained: DiaSys = 0.99 Siemens + 0.00. Using Bland-Altman analysis, the mean bias was –0.004 mg/L on the analytical range between 0.02 and 1 mg/L. Median u-CysC in 50 HIV patients with tubular dysfunction and in 50 patients with AKI was higher than in control subjects. Conclusions Both Siemens and DiaSys reagents demonstrated reliable and reproducible performances allowing easy determination of u-CysC on automated platforms in clinical practice with potential interest for the detection of tubular dysfunction.

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Measurement of cystatin C in human urine by particle-enhanced turbidimetric immunoassay on an automated biochemistry analyzer

Cited by 12 publications

References 14 publications

Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

Novel urinary biomarkers in pre-diabetic nephropathy

Analytical performances of PENIA and PETIA urinary cystatin C determination allow tubular injury investigation

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