Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA

Mesbah, Mostafa K.; Whitman, William B.

doi:10.1016/s0021-9673(01)83344-6

Cited by 319 publications

(241 citation statements)

References 18 publications

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“…The purified DNA was hydrolysed with P1 nuclease and the nucleotides were dephosphorylated with calf intestine alkaline phosphatase (Mesbah & Whitman, 1989). The G+C content of the resulting deoxyribonucleosides was determined by reversed-phase HPLC and calculated from the deoxyguanosine/thymidine ratio (Mesbah & Whitman, 1989).…”

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confidence: 99%

Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

Wang

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae A Gram-negative, aerobic, neutrophilic, coccoid bacterium, strain E4A9 T , was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region. 16S rRNA gene sequence analysis showed that the isolate was related to the type strain of Altererythrobacter epoxidivorans (96.0 % sequence similarity). Lower 16S rRNA gene sequence similarities were observed with other members of the genera Altererythrobacter (94.7 %), Erythrobacter (94.0-95.4 %), Erythromicrobium (94.8 %) and Porphyrobacter (94.6-95.1 %) of the family Erythrobacteraceae. Phylogenetic analysis including all described species of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a distinct phylogenetic lineage with the family Erythrobacteraceae. Chemotaxonomic analysis revealed ubiquinone-10 as the predominant respiratory quinone, anteiso-C 15 : 0 , iso-C 14 : 0 and iso-C 15 : 0 as major fatty acids, and phosphatidylglycerol as the major polar lipid. The DNA G+C content was 71.5 mol%. The isolate contained carotenoids, but no bacteriochlorophyll a. On the basis of phenotypic and genotypic data presented in this study, strain E4A9 T represents a novel species in a new genus in the family Erythrobacteraceae for which the name Croceicoccus marinus gen. nov., sp. nov. is proposed; the type strain is E4A9 T (5CGMCC 1.6776 T 5JCM 14846 T ).The family Erythrobacteraceae (order Sphingomonadales, class Alphaproteobacteria) was proposed by Lee et al. (2005) based on a comprehensive phylogenetic analysis and, at present, it comprises four recognized genera: Altererythrobacter (Kwon et al., 2007), Erythrobacter (Shiba & Simidu, 1982), Erythromicrobium (Yurkov et al., 1994) and Porphyrobacter (Fuerst et al., 1993).Members of the family Erythrobacteraceae are aerobic and produce pink, orange or yellow pigments. Ubiquinone-10 is the major respiratory quinone. Most species in the family have been isolated from various aquatic environments such as freshwater, seawater, marine mats or sediment, a hot spring, seaweed, a starfish and coral (Shiba & Simidu, 1982; Fuerst et al., 1993;Yurkov et al., 1994; Hanada et al., 1997; Denner et al., 2002; Hiraishi et al., 2002;Rainey et al., 2003;Yoon et al., 2003Yoon et al., , 2004a Yoon et al., , b, 2005a Yoon et al., , b, 2006Ivanova et al., 2005;Kwon et al., 2007). This study focuses on the description of strain E4A9 T , isolated from a deep-sea sediment sample. Based on the taxonomic data, it is proposed that this strain be included in a new genus within the family Erythrobacteraceae.The deep-sea sediment samples were collected by a multicorer from the East Pacific polymetallic nodule region (8 u 229 380 N 145 u 239 560 W) at a depth of Abbreviation: BChl a, bacteriochlorophyll a.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain E4A9 T is EF62399...

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Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

Wang

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The G+C content of the DNA was obtained by HPLC, as described by Mesbah & Whitman (1989). Bacterial DNA was isolated using a Qiagen DNAeasy tissue kit; l phage DNA was used as a control.…”

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confidence: 99%

Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs

Chen

Lin

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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Two novel bacteria, with an optimum growth temperature of approximately 60 6C, were isolated from Lu-shan hot springs in the central region of Taiwan. These isolates were aerobic, thermophilic, halotolerant, pink-pigmented, heterotrophic and resistant to gamma-radiation. Both pleomorphic, short, rod-shaped cells and coccoid cells were observed. Strains LS-286 (=ATCC BAA-452=BCRC 17198) and LS-293 T (=ATCC BAA-406 T =BCRC 17173 T ) represented a novel species of the genus Rubrobacter, according to a phylogenetic analysis of the 16S rRNA gene, DNA-DNA hybridization, biochemical features and fatty acid composition. The name Rubrobacter taiwanensis sp. nov. is proposed for this novel species, with LS-293 T as the type strain.

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“…Sporulation was also tested with the heat test and alcohol test as described in Engelkirk et al (1992b). Determination of the GjC content of DNA was performed according to Mesbah & Whitman (1989).…”

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confidence: 99%

Turicibacter sanguinis gen. nov., sp. nov., a novel anaerobic, Gram-positive bacterium.

Bosshard¹,

Zbinden²,

Altwegg³

2002

International Journal of Systematic and Evolutionary Microbiology

145

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Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA

Cited by 319 publications

References 18 publications

Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs

Turicibacter sanguinis gen. nov., sp. nov., a novel anaerobic, Gram-positive bacterium.

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