Measurement of Enolase Activity in Cell Lysates

The Hybrid Pyrroloisoindolone–Dehydropyrrolizine Alkaloid (−)‐Chlorizidine A Targets Proteins within the Glycolytic Pathway

“…This suggestion is further supported by the immunoprecipitation of hENO1 and GAPDH by probe 8 , as well as by the fact that probe 8 , hENO1[14] and GAPDH[15] localized within the cytosol. The identification of HSP90 (Fig.…”

“…In addition, this discovery suggests that 1 or stabilized derivatives such as 2 might serve as tools to study the role of hENO1 and GAPDHi nt umor cell growth and progression further.T his suggestion is supported by the immunoprecipitation of hENO1 and GAPDH by probe 8,a sw ell as by the fact that probe 8,h ENO1 [14] and GAPDH [15] all localized within the cytosol. The identification of HSP90 and actin ( Figure 2C and D), observed in lesser quantities during the target identification process, could suggest further interactivity between hENO1 or GAPDH and cytoskeletal [16] or heat shock [17] proteins.A lternatively,a nd most likely,t he isolation of HSP90 and actin, two abundant proteins in mammalian cells, could have resulted from nonspecific bindinge ventsb ased upon the nucleophilic reactivity of the C5 carbonyl in probe 8.…”

Neuron specific enolase: a promising therapeutic target in acute spinal cord injury

“…The reaction is reversible, depending on environmental concentrations of substrates. In addition to its glycolytic function, enolase plays a variety of roles in pathophysiological settings including ischemia, oncogenesis, tumor progression, and bacterial infection [6]. Enolase exists as cell-type specific isoforms such as αα in most adult tissues, αγ and γγ in neurons, and αβ and ββ in muscle cells.…”

“…Enolase plays multiple roles in growth control, hypoxia, immune tolerance, and allergic responses [4-6]. Enolase can stimulate immunoglobulin production, which in turn may activate humoral and cellular immune responses in the host [7].…”

Octameric structure ofStaphylococcus aureusenolase in complex with phosphoenolpyruvate

“…Despite the absence of a signalling sequence, localization of enolase to the surface of prokaryotic and eukaryotic cells has been reported; however, the transport mechanism remains unknown (Seweryn et al, 2007). Surface enolase has been confirmed as a plasminogen receptor in pathogenic streptococci (Feng et al, 2009), haematopoietic cells, neuronal cells and endothelial cells (Fukano & Kimura, 2014), and is involved in regulating pericellular fibrinolytic activity and extracellular matrix degradation, enhancing monocyte migration and promoting cell migration in cancer metastasis (Capello et al, 2011;Wygrecka et al, 2009;Godier & Hunt, 2013). Additionally, eukaryotic enolase exhibits various subcellular localizations and functions related to pathophysiologies including cancer, apoptosis, arthritis and Alzheimer's disease (Díaz-Ramos et al, 2012).…”