Measurement of Fibrosis in Needle Liver Biopsies: Evaluation of A Colorimetric Method

Jiménez, Wladimiro; Parés, Albert; Caballería, Joan; Heredia, D.; Bruguera, M; Torres, Miguel; Rojkind, Marcos; Rodés, Joan

doi:10.1002/hep.1840050517

Cited by 123 publications

(81 citation statements)

References 11 publications

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“…We have published a method using computer-assisted digital image analysis (DIA) using picroSirius red stained histological sections to quantify liver collagen [1,4], as the quantity of picroSirius red correlates well with morphometrically calculated hepatic fibrosis [5]. The quantitative assessment of collagen is evaluated as collagen proportionate area.…”

Section: Introductionmentioning

confidence: 99%

Digital image analysis of collagen assessment of progression of fibrosis in recurrent HCV after liver transplantation

Manousou¹,

Burroughs²,

Sochatzis³

et al. 2013

Journal of Hepatology

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Section: Introductionmentioning

confidence: 99%

Digital image analysis of collagen assessment of progression of fibrosis in recurrent HCV after liver transplantation

Manousou¹,

Burroughs²,

Sochatzis³

et al. 2013

Journal of Hepatology

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“…Prior studies have shown that morphometry is more sensitive for detecting changes in total hepatic fibrosis during antiviral therapy compared to light microscopy and strongly correlates with chemical determination of liver hydroxyproline content. 23,26,29,37,38 Morphometric analysis of hepatic collagen content, however, is also limited by potential sampling variability and morphometry is unreliable in subjects with fragmented biopsies. The correlation between Ishak fibrosis scores and log-transformed morphometry scores was significant but substantial overlap among individual fibrosis stages was apparent indicating that these measurements are not the same (Fig.…”

Section: Discussionmentioning

confidence: 99%

Relationship of serum fibrosis markers with liver fibrosis stage and collagen content in patients with advanced chronic hepatitis C

Fontana¹,

Goodman²,

Dienstag³

et al. 2008

Hepatology

165

117

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This study determined the utility of a panel of serum fibrosis markers along with routine laboratory tests in estimating the likelihood of histological cirrhosis in a cohort of prior nonresponders with chronic hepatitis C. The relationship between serum markers and quantitative hepatic collagen content was also determined. Liver biopsy samples from 513 subjects enrolled in the HALT-C trial were assigned Ishak fibrosis scores. The collagen content of 386 sirius-red stained, nonfragmented biopsy samples was quantified using computerized morphometry. Serum tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), amino-terminal peptide of type III procollagen (PIIINP), hyaluronic acid (HA), and YKL-40 levels were determined using commercially available assays.Sixty-two percent of patients had noncirrhotic fibrosis (Ishak stage 2-4) whereas 38% had cirrhosis (

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“…Sections of each biopsy stained with Sirius red were used for measurement of fibrosis. This stain binds to all connective tissue, but primarily to collagen, and the quantity of bound stain has been shown to correlate well with chemically determined collagen content and morphometrically determined hepatic fibrosis, 29,30 so the degree of red staining can be taken as proportional to the amount of collagen present. Two sets of images representing the entire biopsy were acquired using a 4ϫ objective.…”

Section: Methodsmentioning

confidence: 99%

Progression of fibrosis in advanced chronic hepatitis C

et al. 2007

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Fibrosis progression in chronic liver disease has usually been evaluated by liver biopsy using insensitive semiquantitative numerical scores. An alternative to this is to measure fibrous tissue quantitatively using morphometric image analysis. The aim of this study was to quantify fibrosis progression in a cohort of patients with treatment-refractory chronic hepatitis C enrolled in a placebo-controlled clinical trial of interferon gamma-1b (IFN-␥ 1b) for the treatment of advanced hepatic fibrosis. We used morphometry to quantify the amount of fibrous tissue in liver biopsies performed at baseline and after 48 weeks in 245 patients who had paired unfragmented, adequate-sized specimens and correlated the results with clinical and laboratory parameters. Eighty-seven patients were treated with placebo and 158 with IFN-␥ 1b. No effect of the drug on fibrosis was found in the trial, and so data from all 245 patients were combined for analysis. At baseline, 78% had cirrhosis; 22%, bridging fibrosis. The mean morphometrically determined collagen content increased by 58% between baseline and 48 weeks. There were statistically significant but weak correlations of fibrosis with platelet count, albumin, bilirubin, INR, and hyaluronic acid; however, changes in these did not correlate with or predict changes in fibrosis in the liver biopsy. Conclusion: In advanced chronic hepatitis C, fibrosis increases at a rapid pace that can only be detected by morphometry. This technique can be used in future therapeutic trials of agents to inhibit fibrosis progression. (HEPATOLOGY 2007;45:886-894.)

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Measurement of Fibrosis in Needle Liver Biopsies: Evaluation of A Colorimetric Method

Cited by 123 publications

References 11 publications

Digital image analysis of collagen assessment of progression of fibrosis in recurrent HCV after liver transplantation

Digital image analysis of collagen assessment of progression of fibrosis in recurrent HCV after liver transplantation

Relationship of serum fibrosis markers with liver fibrosis stage and collagen content in patients with advanced chronic hepatitis C

Progression of fibrosis in advanced chronic hepatitis C

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